Detailed information    

experimental Experimentally validated

Overview

Name   vicK   Type   Regulator
Locus tag   SMU_RS06880 Genome accession   NC_004350
Coordinates   1444856..1446208 (-) Length   450 a.a.
NCBI ID   WP_002262929.1    Uniprot ID   -
Organism   Streptococcus mutans UA159     
Function   repress comCDE expression; repress comX expression   
Competence regulation

Function

Overexpression of the vic operon caused growth-phase-dependent repression of comC, comDE, and comX.


Genomic Context

Location: 1439856..1451208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS06870 (SMU.1514) rnc 1443254..1443949 (-) 696 WP_002262927.1 ribonuclease III -
  SMU_RS06875 (SMU.1515) vicX 1444056..1444859 (-) 804 WP_002262928.1 MBL fold metallo-hydrolase Regulator
  SMU_RS06880 (SMU.1516) vicK 1444856..1446208 (-) 1353 WP_002262929.1 cell wall metabolism sensor histidine kinase VicK Regulator
  SMU_RS06885 (SMU.1517) vicR 1446201..1446908 (-) 708 WP_002262930.1 response regulator YycF Regulator
  SMU_RS06890 (SMU.1519) - 1447261..1448043 (+) 783 WP_002262931.1 amino acid ABC transporter ATP-binding protein -
  SMU_RS06895 (SMU.1520) - 1448040..1448852 (+) 813 WP_002262932.1 transporter substrate-binding domain-containing protein -
  SMU_RS06900 (SMU.1521) - 1448870..1449574 (+) 705 WP_002262933.1 amino acid ABC transporter permease -
  SMU_RS06905 (SMU.1522) - 1449589..1450239 (+) 651 WP_002262934.1 amino acid ABC transporter permease -
  SMU_RS06910 (SMU.1523) endA 1450307..1451188 (-) 882 WP_002262935.1 DNA/RNA non-specific endonuclease Machinery gene

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  vicK comD/blpH negative effect
  comD/blpH comE/blpR positive effect
  comE/blpR comD/blpH positive effect
  comE/blpR comC/blpC positive effect
  comE/blpR comA/nlmT positive effect
  comE/blpR comX/sigX positive effect
  comE/blpR comE/blpR positive effect
  comE/blpR comB/nlmE positive effect
  vicR comE/blpR negative effect
  vicK comE/blpR negative effect
  comD/blpH comX/sigX positive effect
  comX/sigX late competence genes positive effect
  scnR comX/sigX positive effect
  hdrR comX/sigX positive effect
  mecA comX/sigX negative effect
  clpC comX/sigX negative effect
  comR comX/sigX positive effect
  clpP comX/sigX negative effect
  XrpA comX/sigX negative effect
  scnK comX/sigX positive effect
  comS comX/sigX positive effect
  vicK comX/sigX negative effect
  scnC comX/sigX positive effect
  brsR comX/sigX positive effect
  cipB comX/sigX positive effect
  vicR comX/sigX negative effect
  comC/blpC comX/sigX positive effect
  vicR comD/blpH negative effect
  vicR comC/blpC negative effect
  vicK comC/blpC negative effect
  comC/blpC comD/blpH positive effect
  comA/nlmT comC/blpC positive effect
  comB/nlmE comC/blpC positive effect
  ciaH comC/blpC positive effect
  sepM comC/blpC positive effect
  comX/sigX late competence genes positive effect
  hdrR comR positive effect
  hdrM hdrR negative effect
  comR comS positive effect
  cipB comR positive effect
  XrpA comR negative effect
  XrpA comS negative effect
  comS comS positive effect
  oppD comS positive effect
  cipB comS positive effect
  brsM brsR negative effect

Sequence

Protein


Download         Length: 450 a.a.        Molecular weight: 51697.33 Da        Isoelectric Point: 4.4637

>NTDB_id=375 SMU_RS06880 WP_002262929.1 1444856..1446208(-) (vicK) [Streptococcus mutans UA159]
MTNVFESSPLFLRILLAVLIILLFFYFIFLNYREYKNNNQVKQLNAKVRSLITGHYTDKLKVEDNSDLSELVNNVNDLSE
VFRLTHENLAQEKNRLTSILSYMTDGVLATDRSGKITVINDMAQKQLNVTREQALECNILDILDDDSYTYNDLITKTPEI
VLTRRDEYDEFITLRIRFALNRRESGFISGLIAVLHDATEQEKEERERRLFVSNVSHELRTPLTSVKSYLEALDDGALTE
SVAPSFIKVSLDETNRMMRMITDLLSLSRIDNQTSHLDVELTNFTAFMNYILDRFDQIQSQQSTNKVYEIIRDYPDKSVW
IEIDTDKMTQVIDNILNNAIKYSPDGGKVTITMQTTDTQLILSISDQGLGIPKKDLPLIFDRFYRVDKARSRAQGGTGLG
LAIAKEIVKQHKGFIWANSEEGEGSTFTIVLPYENDNDAIDEWEEDEDES

Nucleotide


Download         Length: 1353 bp        

>NTDB_id=375 SMU_RS06880 WP_002262929.1 1444856..1446208(-) (vicK) [Streptococcus mutans UA159]
ATGACTAATGTGTTTGAATCAAGTCCCTTATTTTTACGAATTTTATTAGCAGTTTTAATCATTTTACTCTTTTTTTATTT
TATTTTTCTCAATTACAGAGAGTATAAAAATAATAATCAAGTTAAACAGTTAAATGCTAAAGTACGTTCTTTGATTACTG
GCCATTATACTGATAAATTAAAGGTTGAGGATAACTCTGACTTGTCAGAACTAGTGAATAACGTTAATGATTTATCAGAA
GTTTTCCGTTTAACGCATGAAAATTTGGCACAGGAGAAAAACCGTCTGACTAGTATTTTATCTTATATGACAGATGGTGT
TTTGGCAACAGATCGCAGCGGAAAGATTACCGTTATTAATGATATGGCGCAAAAGCAACTGAATGTAACGCGTGAGCAGG
CATTGGAGTGCAATATTCTGGATATTTTGGATGATGATAGTTATACTTACAATGATTTGATTACAAAAACGCCAGAAATT
GTTCTGACGCGGCGTGATGAATATGATGAATTTATCACTTTACGCATTCGTTTTGCCTTAAACCGCCGTGAAAGTGGCTT
TATTTCAGGACTAATTGCTGTCTTGCATGATGCGACCGAACAGGAAAAAGAAGAACGTGAACGCCGTCTCTTTGTTTCTA
ATGTCAGTCATGAACTGCGGACACCATTAACCTCTGTAAAGTCCTATTTAGAAGCTTTGGACGATGGTGCCCTGACAGAA
TCAGTGGCTCCTAGCTTTATCAAGGTATCACTTGATGAGACTAATCGCATGATGCGTATGATTACTGATTTACTTAGCTT
ATCACGTATTGATAATCAGACCAGTCATCTAGATGTTGAATTGACGAATTTTACAGCTTTTATGAATTATATTCTGGATC
GCTTTGATCAAATTCAAAGTCAGCAATCAACTAATAAGGTTTACGAAATTATTAGAGATTATCCTGACAAATCAGTTTGG
ATTGAAATTGATACCGATAAAATGACTCAGGTAATTGATAATATTTTAAATAATGCAATCAAATATTCACCTGATGGCGG
TAAGGTGACTATAACGATGCAGACAACAGATACACAACTCATCCTTTCTATATCCGACCAAGGCTTGGGTATTCCTAAGA
AGGATCTCCCTCTTATTTTTGACCGTTTTTATCGTGTTGATAAGGCAAGAAGCCGTGCTCAAGGTGGGACTGGTTTAGGA
TTAGCAATCGCCAAAGAAATTGTCAAACAGCATAAAGGCTTTATTTGGGCCAATAGTGAGGAAGGCGAAGGGTCGACCTT
TACCATCGTTTTACCTTATGAAAACGATAATGATGCAATTGATGAATGGGAAGAAGATGAAGACGAATCATGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(208-272)

PAS fold (PAS)

(96-180)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(324-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  micB Streptococcus pneumoniae Cp1015

67.133

95.333

0.64

Multiple sequence alignment    



References

[1] D B Senadheera et al. (2012) Regulation of bacteriocin production and cell death by the VicRK signaling system in Streptococcus mutans. Journal of Bacteriology 194(6):1307-16. [PMID: 22228735]
[2] M Dilani Senadheera et al. (2007) The Streptococcus mutans vicX gene product modulates gtfB/C expression, biofilm formation, genetic competence, and oxidative stress tolerance. Journal of Bacteriology 189(4):1451-8. [PMID: 17114248]
[3] M Dilani Senadheera et al. (2005) A VicRK signal transduction system in Streptococcus mutans affects gtfBCD, gbpB, and ftf expression, biofilm formation, and genetic competence development. Journal of Bacteriology 187(12):4064-76. [PMID: 15937169]