Detailed information NTG0000414

Experimentally validated

Overview

• Name: micB
• Type: Regulator
• Locus tag: -
• Genome accession: AF219111
• Coordinates: 838..2187 (+)
• Length: 449 a.a.
• NCBI ID: AAK55818.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae Cp1015
• Function: repress competence development Competence regulation

Function

The two-component MicAB system, whose MicB kinase carries a PAS domain, is also involved in competence repression under oxygen limitation. Autophosphorylation of recombinant MicB and phosphotransfer to recombinant MicA have been demonstrated.

Genomic Context

Location: 1..7187

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Locus_0	micA	141..845 (+)	705	AAK55817.1	putative response regulator	Regulator
Locus_1	micB	838..2187 (+)	1350	AAK55818.1	putative histidine kinase	Regulator
Locus_2	vicX	2189..2998 (+)	810	AAK55819.1	MicC	Regulator

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51659.37 Da
• Isoelectric Point: 4.9083

>NTDB_id=414 AAK55818.1 838..2187(+) (micB) [Streptococcus pneumoniae Cp1015]
MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQINQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSE
VIRLTQENLEQESKRLNSILFYMTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSHELRTPLTSVKSYLEALDEGALC
ETVAPDFIKVSLDETNRMMRMVTDLLHLSCIDNATSHLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIW
MEIDTDKMTQVVDNILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSRAQGGTGLG
LSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED*

Nucleotide

• Length: 1350 bp

>NTDB_id=414 AAK55818.1 838..2187(+) (micB) [Streptococcus pneumoniae Cp1015]
ATGCTTGATTTACTGAAACAAACCATTTTTACCAGAGATTTTATCTTTATCCTGATTTTGTTAGGTTTCATCCTTGTTGT
GACCCTCTTATTACTGGAAAATAGACGTGATAATATTCAGTTGAAGCAAATCAATCAAAAGGTTAAAGATTTGATTGCAG
GAGATTATTCCAAGGTTCTTGATATGCAAGGTGGGTCTGAAATCACCAATATTACCAATAATTTGAATGACTTGTCGGAG
GTTATTCGTCTCACTCAGGAAAATCTAGAACAAGAGAGTAAGAGGCTAAATAGTATTCTGTTTTATATGACAGATGGGGT
TCTTGCGACTAACCGTCGGGGTCAGATTATCATGATTAACGATACAGCCAAGAAGCAACTGGGGTTGGTTAAGGAAGATG
TTCTGAATAGAAGTATTTTGGAATTGCTCAAGATAGAAGAAAACTATGAATTGCGTGATTTGATTACCCAAAGTCCAGAA
TTGTTGCTAGATTCCCAAGATATCAATGGCGAATATTTGAACCTTCGAGTTCGCTTTGCCTTGATACGTCGAGAGTCTGG
CTTTATTTCAGGTTTGGTGGCTGTTTTGCATGATACGACGGAGCAGGAGAAGGAAGAACGCGAACGAAGACTCTTTGTTT
CCAATGTTAGCCATGAGTTACGGACTCCTCTGACTAGCGTAAAATCCTATCTTGAAGCCTTGGATGAGGGGGCTTTGTGT
GAAACTGTAGCACCAGACTTTATCAAGGTTTCTCTTGATGAGACCAACCGTATGATGCGCATGGTGACGGATCTCCTCCA
TCTTTCATGTATTGATAATGCTACCAGTCACCTAGATGTGGAACTGATTAACTTCACTGCTTTTATTACCTTTATCCTCA
ATCGTTTTGACAAGATGAAAGGACAGGAAAAGGAGAAAAAATATGAGCTGGTGAGAGATTATCCCATCAATTCTATCTGG
ATGGAAATTGATACAGATAAGATGACGCAGGTTGTCGACAATATTTTAAATAATGCTATTAAGTATTCGCCAGATGGGGG
TAAAATCACTGTCAGAATGAAGACAACTGAAGACCAGATGATTTTATCCATTTCTGACCACGGTTTGGGGATTCCTAAGC
AGGATTTACCACGTATCTTTGACCGTTTCTATCGTGTGGATCGTGCTAGAAGTCGTGCACAAGGTGGTACAGGTCTAGGA
CTGTCTATCGCTAAAGAAATTATCAAACAACATAAGGGCTTTATTTGGGCCAAGAGTGAATACGGCAAGGGTTCAACCTT
TACCATTGTACTCCCTTATGATAAGGATGCAGTGAAAGAAGAAGTATGGGAGGATGAAGTAGAAGACTAG

Domains

Predicted by InterproScan.

• His Kinase A (phospho-acceptor) domain (HisKA) (209-273)

• PAS fold (PAS) (98-198)

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (324-434)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
vicK	Streptococcus mutans UA159	67.133	95.333	0.64

References

• [1]: J R Echenique et al. (2001) Competence repression under oxygen limitation through the two-component MicAB signal-transducing system in Streptococcus pneumoniae and involvement of the PAS domain of MicB. Journal of Bacteriology 183(15):4599-608. [PMID: 11443095]