Detailed information    

experimental Experimentally validated

Overview

Name   micA   Type   Regulator
Locus tag   - Genome accession   AF219111
Coordinates   141..845 (+) Length   234 a.a.
NCBI ID   AAK55817.1    Uniprot ID   -
Organism   Streptococcus pneumoniae Cp1015     
Function   repress competence development   
Competence regulation

Function

The two-component MicAB system, whose MicB kinase carries a PAS domain, is also involved in competence repression under oxygen limitation. Autophosphorylation of recombinant MicB and phosphotransfer to recombinant MicA have been demonstrated.


Genomic Context

Location: 1..5845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Locus_0 micA 141..845 (+) 705 AAK55817.1 putative response regulator Regulator
  Locus_1 micB 838..2187 (+) 1350 AAK55818.1 putative histidine kinase Regulator
  Locus_2 vicX 2189..2998 (+) 810 AAK55819.1 MicC Regulator

Sequence

Protein


Download         Length: 234 a.a.        Molecular weight: 26815.63 Da        Isoelectric Point: 4.7164

>NTDB_id=413 AAK55817.1 141..845(+) (micA) [Streptococcus pneumoniae Cp1015]
MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAEQPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSA
KDSEFDKVIGLELGADDYVTKPFSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPEYILTRRGVGYYMRNNA*

Nucleotide


Download         Length: 705 bp        

>NTDB_id=413 AAK55817.1 141..845(+) (micA) [Streptococcus pneumoniae Cp1015]
ATGAAAAAAATACTAATTGTAGATGATGAGAAACCAATCTCGGATATTATCAAGTTTAATATGACCAAGGAAGGTTATGA
AGTTGTAACTGCTTTTAATGGTCGTGAAGCGCTAGAGCAATTTGAAGCAGAGCAACCAGATATTATTATTCTGGATTTGA
TGCTTCCAGAAATTGATGGTTTAGAAGTTGCTAAGACCATTCGTAAGACAAGCAGTGTGCCCATTCTTATGCTTTCAGCC
AAAGATAGTGAATTTGATAAGGTTATCGGTTTGGAACTTGGGGCAGATGACTATGTAACGAAACCCTTCTCCAATCGTGA
GTTGCAGGCGCGTGTTAAAGCTCTTCTGCGTCGTTCTCAACCTATGCCAGTAGATGGTCAGGAAGCAGATAGTAAACCTC
AACCTATCCAAATTGGGGATTTAGAAATTGTTCCAGACGCCTACGTGGCTAAAAAATATGGCGAAGAACTAGACTTAACC
CATCGTGAATTTGAGCTTTTGTATCATTTAGCATCGCATACAGGTCAAGTCATCACGCGCGAACACTTGCTTGAGACTGT
CTGGGGTTATGACTATTTTGGTGATGTCCGCACAGTTGATGTGACTGTACGACGTCTGCGTGAGAAGATTGAAGATACGC
CCAGCCGACCAGAGTATATCTTGACGCGCCGTGGTGTAGGGTATTACATGAGAAATAATGCTTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(4-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(154-229)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  vicR Streptococcus mutans UA159

78.541

99.149

0.779
  covR Streptococcus salivarius strain HSISS4

44.156

100

0.445
  covR Lactococcus lactis subsp. lactis strain DGCC12653

43.478

100

0.435
  scnR Streptococcus mutans UA159

38.197

100

0.389
  pilH Synechocystis sp. PCC 6803

38.333

99.174

0.38

Multiple sequence alignment    



References

[1] J R Echenique et al. (2001) Competence repression under oxygen limitation through the two-component MicAB signal-transducing system in Streptococcus pneumoniae and involvement of the PAS domain of MicB. Journal of Bacteriology 183(15):4599-608. [PMID: 11443095]