Detailed information    

experimental Experimentally validated

Overview

Name   pilH   Type   Machinery gene
Locus tag   SGL_RS04200 Genome accession   NC_000911
Coordinates   491084..491449 (+) Length   121 a.a.
NCBI ID   WP_014407071.1    Uniprot ID   -
Organism   Synechocystis sp. PCC 6803     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

Competency of the pilH mutant, which lost motility but accumulated a greater amount of the thick pili, the transformation efficiency was about one-third of wild type.


Genomic Context

Location: 486084..496449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGL_RS04185 (sll1500) cobT 486244..487353 (-) 1110 WP_010871651.1 nicotinate mononucleotide-dependent phosphoribosyltransferase CobT -
  SGL_RS04190 (slr1609) - 487467..489377 (+) 1911 WP_014407070.1 AMP-dependent synthetase/ligase -
  SGL_RS04195 (slr1041) - 489722..490888 (+) 1167 WP_010871653.1 response regulator -
  SGL_RS04200 (slr1042) pilH 491084..491449 (+) 366 WP_014407071.1 response regulator transcription factor Machinery gene
  SGL_RS04205 (slr1043) - 491496..492059 (+) 564 WP_010871655.1 chemotaxis protein CheW -
  SGL_RS04210 (slr1044) pilJ 492130..494739 (+) 2610 WP_010871656.1 methyl-accepting chemotaxis protein Machinery gene
  SGL_RS04215 (slr1045) - 494828..495619 (+) 792 WP_010871657.1 ABC transporter permease -
  SGL_RS04220 (slr1046) - 495749..496006 (+) 258 WP_041425909.1 TatA/E family twin arginine-targeting protein translocase -

Sequence

Protein


Download         Length: 121 a.a.        Molecular weight: 13674.90 Da        Isoelectric Point: 5.8739

>NTDB_id=1417 SGL_RS04200 WP_014407071.1 491084..491449(+) (pilH) [Synechocystis sp. PCC 6803]
MNAVLLVEDSSSQREMISGILKDHGWQVTIACDGVEALEKLQNFSPDLVVLDIVMPRMNGYEVCRRIKSDPKTKNVPVIM
CSSKGEEFDRFWGMRQGADAYIAKPFQPMELVGTIKQLLRN

Nucleotide


Download         Length: 366 bp        

>NTDB_id=1417 SGL_RS04200 WP_014407071.1 491084..491449(+) (pilH) [Synechocystis sp. PCC 6803]
ATGAACGCAGTTTTGCTGGTTGAAGACAGCTCTAGTCAGCGCGAGATGATATCTGGCATCCTCAAAGACCATGGCTGGCA
GGTCACCATCGCCTGTGATGGGGTGGAGGCCCTGGAAAAATTGCAGAATTTTAGTCCGGACCTAGTGGTGCTAGATATTG
TCATGCCCCGCATGAATGGCTATGAGGTTTGTCGACGCATCAAATCCGATCCGAAAACCAAAAATGTGCCAGTGATTATG
TGCTCCTCCAAGGGGGAGGAATTTGACCGTTTTTGGGGCATGCGTCAGGGAGCCGACGCCTACATTGCCAAACCATTTCA
GCCCATGGAACTGGTGGGCACCATCAAACAACTCCTGCGCAATTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(4-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Acinetobacter baumannii strain A118

41.593

93.388

0.388
  chpA Acinetobacter baumannii strain A118

39.655

95.868

0.38
  vicR Streptococcus mutans UA159

38.333

99.174

0.38
  micA Streptococcus pneumoniae Cp1015

38.333

99.174

0.38
  pilL-C Synechocystis sp. PCC 6803

37.5

99.174

0.372

Multiple sequence alignment    



References

[1] Shizue Yoshihara et al. (2002) pilG Gene cluster and split pilL genes involved in pilus biogenesis, motility and genetic transformation in the cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology 43(5):513-21. [PMID: 12040098]