Detailed information NTG0001418

Experimentally validated

Overview

• Name: pilJ
• Type: Machinery gene
• Locus tag: SGL_RS04210
• Genome accession: NC_000911
• Coordinates: 492130..494739 (+)
• Length: 869 a.a.
• NCBI ID: WP_010871656.1
• Uniprot ID: -
• Organism: Synechocystis sp. PCC 6803
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

The pilJ and pilL-C mutants retained very slight competency (1.5% of wild type), although they showed non-motile phenotype and practically non-piliation in terms of the thick pilus structure.

Genomic Context

Location: 487130..499739

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SGL_RS04190 (slr1609)	-	487467..489377 (+)	1911	WP_014407070.1	AMP-dependent synthetase/ligase	-
SGL_RS04195 (slr1041)	-	489722..490888 (+)	1167	WP_010871653.1	response regulator	-
SGL_RS04200 (slr1042)	pilH	491084..491449 (+)	366	WP_014407071.1	response regulator transcription factor	Machinery gene
SGL_RS04205 (slr1043)	-	491496..492059 (+)	564	WP_010871655.1	chemotaxis protein CheW	-
SGL_RS04210 (slr1044)	pilJ	492130..494739 (+)	2610	WP_010871656.1	methyl-accepting chemotaxis protein	Machinery gene
SGL_RS04215 (slr1045)	-	494828..495619 (+)	792	WP_010871657.1	ABC transporter permease	-
SGL_RS04220 (slr1046)	-	495749..496006 (+)	258	WP_041425909.1	TatA/E family twin arginine-targeting protein translocase	-
SGL_RS04225 (slr1047)	-	496450..497844 (+)	1395	WP_010871659.1	sensor domain-containing diguanylate cyclase	-
SGL_RS19430	-	497906..498073 (+)	168	WP_158299069.1	hypothetical protein	-

Sequence

Protein

• Length: 869 a.a.
• Molecular weight: 93209.76 Da
• Isoelectric Point: 4.0166

>NTDB_id=1418 SGL_RS04210 WP_010871656.1 492130..494739(+) (pilJ) [Synechocystis sp. PCC 6803]
MATETNFSQLYGLAYAAYGQGNYQEASSHIEQLAADFPEDPNVLLLRGHIYVGLEQYALAHQAYQGVIRFSDRQDLIDCA
NQALGQIQEEEPRGNGAKDSLDQDWQSDIDAIDNSMAWETGVFTEEDFGEPTLGRAPSPTNPGMDHPFGRSSASFGTSGA
RSYEIENLDWSPEAFTDDLDSDDEQTMMPQTGESTFVVPPAFGEGIADFADLAADVSSAGIGGTDGAEYPPYQTWDGGDW
SEALGLDDPEALAQAPTPTSGDYGAGNNLPSFDAFEEGVEDELSDFNLTDIAPELPDSSLFSQATNLDIGAVGRIDDTQG
GDTVVHGGNAAPPGMATWLKPDGAATGDLSSATSPFNDRPTSEVDAGFLGWFTNASLFRKQIYMAIASGVFSGFAVLVLG
SIVGLGGTPKDVPAPSGETTTEAPAEGAPAEGQAPSQTPEEEPGKPSLLNLAFLTAIATAIGVFLINRLLMQQIKSIIDD
LQNQFESIYEGNFNVRAKVRSEDELGALTQRFNYISQVILTATSEAQERAATTEKVREKLQRQVIRLLDDVEGASRGDLT
VQAEVTADELGAVADAFNLTIQNLREIVLQVKNAAKQVNNNCKDSESFARNNSSDALRMAEELAVTLNSVQLMTESIERV
AENAREAEEVAHTSSLTALKGGEAVERTVGGILQIRETVSETARKVKRLAEASQEISKIVAVISQIASRTNLLALNASIQ
AARAGEAGRGFAIVADEVRQLADRSAKSLKEIEQIVLQIQSETGSVMTAMEEGIQQVIDVTDKSEQSKRALEDIIEVSNR
INTLVRSITADTVKQQENSGAVSNVMQSIELTAQETSQESQRVAGSLQTLVSISRDLLTSVERFRVESR

Nucleotide

• Length: 2610 bp

>NTDB_id=1418 SGL_RS04210 WP_010871656.1 492130..494739(+) (pilJ) [Synechocystis sp. PCC 6803]
ATGGCAACAGAAACCAATTTTTCGCAGCTTTATGGGTTGGCCTACGCCGCCTATGGACAGGGGAATTATCAAGAAGCTTC
TTCCCATATTGAACAATTGGCCGCTGATTTTCCCGAAGATCCTAATGTATTGTTGCTCCGGGGGCATATCTATGTCGGCC
TGGAACAATACGCCCTAGCCCACCAGGCTTATCAGGGGGTAATTCGGTTTTCCGATCGCCAAGACTTAATTGACTGCGCT
AACCAGGCCCTAGGGCAAATCCAAGAAGAGGAACCAAGGGGCAACGGAGCCAAAGATTCCCTTGACCAGGATTGGCAAAG
TGACATCGATGCCATTGACAATTCCATGGCCTGGGAAACAGGAGTGTTCACCGAAGAGGATTTCGGCGAGCCCACTTTGG
GCCGGGCCCCCAGCCCCACTAATCCGGGCATGGACCATCCCTTTGGCCGCAGCTCTGCTTCCTTTGGTACCAGTGGGGCC
AGGAGTTACGAAATTGAAAACCTAGATTGGAGTCCGGAAGCTTTCACCGATGACCTGGACTCCGATGATGAACAGACCAT
GATGCCCCAAACTGGCGAGTCTACTTTTGTGGTGCCACCGGCCTTTGGTGAAGGGATAGCGGATTTTGCCGATTTAGCGG
CGGATGTCTCCTCAGCCGGCATTGGCGGGACAGATGGTGCCGAATATCCCCCATATCAAACTTGGGACGGGGGCGACTGG
AGTGAAGCTCTCGGTTTGGATGATCCTGAAGCCTTGGCTCAGGCCCCTACCCCCACCTCTGGCGATTACGGCGCTGGCAA
TAACCTACCGAGTTTTGATGCGTTTGAGGAAGGGGTGGAGGATGAGTTGTCCGATTTTAACCTGACGGATATTGCCCCAG
AGTTGCCCGATTCTTCCCTCTTTAGCCAAGCCACTAATTTGGACATAGGTGCGGTGGGTCGCATCGATGACACCCAGGGC
GGTGATACGGTGGTCCATGGAGGTAATGCAGCGCCACCGGGCATGGCCACTTGGTTGAAACCGGATGGGGCCGCCACCGG
GGATTTATCCAGCGCCACTAGTCCCTTTAATGACCGGCCCACCAGTGAGGTAGATGCGGGTTTTCTCGGTTGGTTCACCA
ATGCTTCCCTGTTTAGAAAGCAGATTTACATGGCGATCGCCTCCGGTGTGTTTTCCGGGTTCGCGGTGTTGGTATTGGGA
AGCATAGTGGGTTTAGGGGGAACTCCCAAAGACGTACCTGCTCCTTCAGGGGAAACCACCACCGAAGCTCCAGCAGAAGG
TGCCCCCGCAGAGGGCCAGGCCCCTTCCCAGACCCCAGAGGAAGAACCGGGCAAACCATCCCTCCTCAACCTCGCCTTCC
TCACAGCCATAGCCACGGCGATCGGAGTCTTTCTGATCAATCGATTGCTGATGCAACAGATTAAGAGTATTATCGACGAC
CTGCAAAATCAGTTTGAATCCATCTATGAGGGCAATTTCAATGTCCGGGCCAAGGTGCGATCGGAGGATGAATTGGGGGC
TTTGACCCAACGGTTTAACTATATTTCCCAAGTTATTCTCACGGCCACTAGTGAAGCCCAGGAACGGGCTGCTACCACCG
AAAAAGTGCGGGAAAAATTACAGCGCCAAGTAATTCGGCTCCTGGACGATGTAGAGGGAGCTTCCCGGGGAGATTTAACC
GTACAAGCGGAAGTAACGGCGGATGAGCTGGGAGCGGTGGCCGATGCCTTTAACCTAACTATCCAAAACCTGCGGGAGAT
TGTGTTACAAGTTAAAAATGCCGCCAAGCAGGTGAATAACAACTGTAAAGACAGTGAGTCCTTTGCCCGCAATAACTCCA
GCGATGCGTTGCGAATGGCGGAGGAATTGGCCGTCACCCTCAACTCTGTTCAGTTAATGACGGAATCCATTGAACGGGTA
GCAGAAAACGCCCGGGAAGCGGAGGAAGTGGCCCATACTTCATCTTTAACTGCCCTCAAAGGTGGGGAAGCGGTGGAAAG
AACGGTGGGGGGCATTCTGCAAATTCGGGAAACGGTGTCGGAAACTGCCCGCAAGGTAAAACGGTTAGCGGAAGCATCCC
AGGAAATTTCCAAAATTGTGGCGGTTATTTCCCAAATTGCCTCCCGGACTAACCTGTTAGCTTTGAACGCCTCCATCCAG
GCGGCCCGGGCAGGGGAAGCAGGGCGGGGCTTTGCCATCGTTGCAGACGAGGTGCGACAGTTGGCGGATCGTTCTGCTAA
GTCGTTGAAGGAAATTGAGCAAATCGTTTTGCAAATTCAGAGTGAAACCGGCTCCGTAATGACCGCCATGGAAGAAGGTA
TCCAACAGGTAATCGACGTAACTGACAAGTCGGAACAGTCGAAACGGGCCCTGGAGGATATTATTGAGGTGTCTAATCGG
ATTAACACTTTGGTGCGTTCCATTACTGCTGATACCGTCAAGCAGCAGGAGAACTCCGGTGCAGTGTCCAACGTCATGCA
GTCCATTGAGCTGACGGCCCAGGAAACTTCCCAGGAATCTCAACGGGTAGCAGGATCTTTGCAAACCCTGGTCTCCATTT
CCCGCGACCTGTTAACTTCGGTGGAACGGTTCCGGGTGGAAAGTCGCTAG

Domains

Predicted by InterproScan.

• HAMP domain (HAMP) (555-586)

• HAMP domain (HAMP) (473-514)

• Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal) (649-830)

References

• [1]: Shizue Yoshihara et al. (2002) pilG Gene cluster and split pilL genes involved in pilus biogenesis, motility and genetic transformation in the cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology 43(5):513-21. [PMID: 12040098]