Detailed information    

experimental Experimentally validated

Overview

Name   chpA   Type   Regulator
Locus tag   H0N27_RS03130 Genome accession   NZ_CP059039
Coordinates   651287..655807 (+) Length   1506 a.a.
NCBI ID   WP_168726941.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain A118     
Function   regulation of type IV pilus assembly   
Competence regulation

Function

In contrast to A. baylyi, deletion of pilS or pilR as well as chpA or pilG in A. baumannii completely abolished transformation, while the absence of pilH did not change the strain’s transformation efficiency


Genomic Context

Location: 646287..660807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0N27_RS03105 (H0N27_03105) - 646728..647366 (-) 639 WP_171945457.1 hypothetical protein -
  H0N27_RS03110 (H0N27_03110) pilG 647649..648032 (+) 384 WP_000389061.1 twitching motility response regulator PilG Regulator
  H0N27_RS03115 (H0N27_03115) - 648056..648418 (+) 363 WP_000101096.1 PleD family two-component system response regulator -
  H0N27_RS03120 (H0N27_03120) - 648479..649015 (+) 537 WP_000729762.1 chemotaxis protein CheW -
  H0N27_RS03125 (H0N27_03125) - 649062..651140 (+) 2079 WP_168726940.1 methyl-accepting chemotaxis protein -
  H0N27_RS03130 (H0N27_03130) chpA 651287..655807 (+) 4521 WP_168726941.1 Hpt domain-containing protein Regulator
  H0N27_RS03135 (H0N27_03135) - 655804..656268 (+) 465 WP_004745996.1 hypothetical protein -
  H0N27_RS03140 (H0N27_03140) dapE 656304..657440 (-) 1137 WP_168726942.1 succinyl-diaminopimelate desuccinylase -
  H0N27_RS03145 (H0N27_03145) - 657566..657709 (-) 144 WP_001984826.1 entericidin A/B family lipoprotein -
  H0N27_RS03150 (H0N27_03150) - 657808..658716 (-) 909 WP_031963197.1 DUF1853 family protein -
  H0N27_RS03155 (H0N27_03155) - 658821..659189 (+) 369 WP_001259501.1 YbaN family protein -
  H0N27_RS03160 (H0N27_03160) elsL 659340..659843 (+) 504 WP_000077304.1 cell wall-recycling L,D-carboxypeptidase ElsL -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  chpA pilG positive effect

Sequence

Protein


Download         Length: 1506 a.a.        Molecular weight: 171812.19 Da        Isoelectric Point: 4.3297

>NTDB_id=1046 H0N27_RS03130 WP_168726941.1 651287..655807(+) (chpA) [Acinetobacter baumannii strain A118]
MKEILKTLTEAMTLPEDSYSEQDAEFLEIFIEEIEEIFVDLQPLINKWMQSENIATLTEIRRHFHTLKGSGRMIGAKSSA
ELAWTVEDTLNRVINQSLQLTPTIQSYVQLVFKFYFLKLVDNFKQKRAHTLDFRPLILLGQQLQQQQSLEPALEELLQLS
HTLMAETVTGLELDYIEQDSLAEPIIQATHTEIEINLDETLTLFMEEAEEHLATIHQFLDQELHQYDSYNALIRALHTLR
GSSAMAQVETIFEASTKVEHLFKILLQEELSSHSEEILLLQEYREFVRDSLELLSRYSSSEQLEARLLQFNQSWDAYVEQ
HGDRTDPLMPSHGLVSQLLQLDVSELLDAELDFEKGIRNEFPDYLERLSEQADLLLQHTHSQAMLGLHEYTSQLKESYEV
LLDKPALLQSDYIFEIYQKAHQQLIQLFDALAAGQRVGVVKQHQSILEELKLYTQYTPDISNDLPQQDSTSFEPIYNIEP
EPEVVATVENFSDSADWAVLGQSVQQDRQYISSTQVNRNFDADLLDIFLEEAEELLEGIDTDLNIWVGEQENFAALNNLM
RYLHTLKGGANMVQATYLGLIAHELESIYERLIQKQLVVTSDLIDFIRLVQDDLADRLQIMREQQLDYAAPYTINALKRA
GQNSNFQPLPVVEAFDAESEVFSEQKVISEIIIDEIPVELEPALAELETHHDQVFDTAVTELATPVEMITTVTSQENEVA
ANEQDIEAVVEQTFLEEATELLEMAESLLKQWFEQRTNRSILLQLQRAVHSLKGGARMVGLEAVQAIAYQLENAFEQFAL
HHFNSNIYDHLLESAIAWLKDAIFNHNYQHFDGLQQSLENIQFFETTIQIPTKLTRADLFSSEPVMTFIQGDGTEPPPMM
GAWEQTERLDQNNEMIRVSADLIEKMIDLSGENSINRSRIEMDLSQFSHTLVEMELAIQRLADQLRRMEGELETQIIAKH
GIEHSRYTDFDPLEMDQYSSLNQLSKSLAESASDLVDFKNTLSDKIRDTESLLLQQSRIQAEIQEGLMRTRLVPFSRLLP
RLQRIVRQTSTALNRPAELFVNNTEGELDRNILERLVTPLEHMLRNAIDHGLEDRAQRQQANKPETGRIELNIQRQGTDV
VVVFSDDGQGIDVEKVRQKALLAGLIKPEQDLEQQDILQLIFHPGLSTAEQVTQISGRGVGLDVVQSDIKSLGGHVSVES
VYGQGTTFTIRVPTTVAVSDALMVKVADQQFAIPLAQIDRIIRVSPASLEQYFESPQELFEYENKRYPLRYLSEFVGNQP
IPRLNGVMYSLPVLMIKANNGQTVALLVDQLIGSRAQIVVKPIGQQFSSIGAIAGATILGDGQVCLILDGQNIARQIQST
QRHKQLSEAVYRQRESDERRLIMIVDDSVTVRKVTSRLLERQGYDVVTAKDGVDAIEQLENIKPDLMLLDIEMPRMDGFE
VLNLVRHHDMHQYMPIIMITSRTGEKHRERAFSLGVSQYMGKPFQEEELLENIDALLVAFESEVKS

Nucleotide


Download         Length: 4521 bp        

>NTDB_id=1046 H0N27_RS03130 WP_168726941.1 651287..655807(+) (chpA) [Acinetobacter baumannii strain A118]
ATGAAAGAAATATTAAAAACTTTAACTGAAGCAATGACGCTACCTGAAGATAGCTATTCTGAACAAGATGCTGAGTTTCT
TGAGATCTTCATTGAAGAAATTGAAGAGATTTTTGTTGATTTGCAACCTTTGATAAATAAATGGATGCAGTCTGAGAATA
TTGCTACGCTCACTGAAATTCGTCGTCATTTCCATACCTTAAAAGGTTCCGGAAGAATGATTGGCGCTAAATCTTCAGCT
GAGCTTGCGTGGACAGTTGAAGATACACTCAACCGGGTAATCAACCAAAGCTTACAATTAACGCCAACTATTCAGAGCTA
TGTGCAGTTAGTATTTAAGTTCTATTTCCTTAAATTAGTAGATAATTTTAAGCAAAAACGAGCACATACCTTAGATTTCA
GACCTCTTATTTTATTAGGCCAACAACTACAACAACAGCAAAGTTTAGAGCCAGCTTTAGAAGAGCTTTTACAACTTTCA
CATACGCTTATGGCCGAAACAGTGACTGGTCTTGAGCTAGATTATATTGAACAAGATTCGTTAGCCGAGCCAATCATTCA
AGCTACACATACTGAAATTGAAATAAATCTAGATGAAACTTTGACCTTATTTATGGAAGAGGCGGAAGAACATTTAGCCA
CGATTCATCAATTCTTAGATCAAGAGTTACATCAGTACGACAGTTATAACGCTTTAATCCGCGCTTTACATACTTTGCGT
GGTAGTTCTGCAATGGCACAGGTCGAAACAATTTTCGAGGCCAGTACCAAAGTTGAGCATTTATTTAAAATACTGCTACA
AGAAGAGTTATCTTCTCACTCTGAAGAAATTTTATTATTACAAGAATATCGTGAGTTTGTTCGAGATAGTTTGGAACTAC
TATCTAGATATAGTTCAAGTGAACAGCTCGAAGCGAGATTGCTGCAATTTAATCAATCTTGGGATGCATATGTTGAGCAA
CATGGTGACCGAACAGATCCGTTAATGCCTTCTCATGGCTTGGTATCGCAATTATTACAATTAGATGTTTCTGAATTATT
GGATGCAGAATTAGATTTTGAGAAGGGAATTCGTAATGAGTTTCCTGATTACCTTGAACGCCTAAGTGAACAGGCTGATT
TATTACTGCAACATACACATTCTCAAGCAATGCTGGGTTTACATGAATATACCAGCCAACTGAAAGAAAGTTATGAAGTG
CTGCTTGATAAACCTGCTTTATTACAATCAGACTATATATTTGAAATTTATCAAAAAGCGCATCAACAGCTCATTCAATT
ATTTGATGCTTTAGCTGCTGGTCAGCGAGTCGGTGTTGTTAAGCAACATCAAAGTATTTTAGAAGAATTAAAACTTTATA
CGCAGTACACTCCAGACATATCAAATGACCTGCCTCAGCAGGATTCGACATCTTTTGAACCAATATACAATATTGAACCA
GAGCCAGAAGTGGTAGCTACTGTAGAGAATTTCTCAGACTCTGCAGACTGGGCGGTATTAGGTCAAAGTGTGCAGCAAGA
TCGACAATATATTTCATCTACACAAGTGAATCGAAATTTTGATGCTGATCTTTTAGATATCTTTCTTGAGGAAGCTGAAG
AATTACTTGAGGGAATTGATACCGATCTTAATATTTGGGTAGGTGAGCAAGAAAACTTTGCAGCTCTGAACAACCTAATG
CGTTATTTGCATACCTTGAAAGGGGGCGCAAATATGGTTCAGGCAACGTATCTTGGTTTGATTGCCCATGAATTAGAAAG
CATCTATGAGCGTTTAATTCAAAAGCAATTAGTGGTAACTTCAGATTTAATTGATTTTATTCGTTTGGTTCAAGATGACT
TGGCTGATCGTTTACAAATCATGCGTGAACAACAACTAGATTATGCAGCTCCTTACACAATTAACGCATTAAAACGAGCG
GGTCAAAACTCTAACTTCCAACCTTTACCAGTGGTTGAGGCATTTGATGCTGAAAGTGAGGTATTCTCTGAGCAAAAGGT
GATATCTGAAATAATAATAGATGAGATACCAGTAGAACTTGAACCTGCTCTTGCAGAGCTAGAAACTCATCACGATCAGG
TATTCGATACAGCGGTTACCGAGTTAGCTACTCCAGTTGAGATGATTACCACGGTTACATCGCAAGAGAATGAAGTTGCA
GCTAATGAACAGGATATTGAGGCTGTTGTTGAACAGACGTTCCTAGAAGAAGCTACTGAATTGTTAGAAATGGCTGAGTC
ACTATTAAAACAATGGTTTGAACAGCGTACAAACCGTAGTATTTTGCTTCAGCTTCAAAGAGCCGTGCACAGTTTAAAAG
GTGGTGCACGGATGGTGGGTTTAGAAGCCGTACAAGCAATTGCCTACCAACTAGAAAATGCTTTTGAACAATTTGCGCTA
CATCACTTCAATTCGAATATTTATGATCATTTATTAGAAAGTGCGATTGCATGGTTAAAAGATGCAATTTTCAACCACAA
CTATCAGCATTTCGATGGATTACAGCAAAGTTTAGAAAATATTCAATTCTTTGAAACTACAATCCAGATTCCGACTAAGT
TAACTAGAGCGGACTTGTTTAGCTCAGAACCAGTGATGACCTTTATACAAGGTGATGGTACAGAGCCTCCACCAATGATG
GGAGCATGGGAGCAAACAGAGCGTCTGGATCAGAATAATGAAATGATCCGTGTCTCAGCCGACTTAATCGAGAAAATGAT
TGATTTGTCTGGTGAAAACTCCATTAACCGTTCACGAATTGAAATGGACCTAAGTCAGTTTAGCCATACACTAGTTGAAA
TGGAGTTGGCGATTCAACGACTTGCCGATCAGTTACGACGAATGGAAGGTGAGTTAGAAACACAGATTATTGCTAAACAT
GGAATTGAACATTCTCGTTATACCGACTTTGATCCGTTAGAGATGGACCAGTATTCATCTTTAAATCAATTGTCGAAATC
GCTTGCTGAATCTGCATCGGACTTGGTCGACTTTAAAAATACGTTATCTGACAAAATCAGAGATACCGAAAGTTTGCTTT
TACAACAATCACGAATTCAAGCGGAAATTCAAGAAGGTTTAATGCGGACACGGTTGGTCCCATTTTCGCGTTTATTGCCG
CGTTTACAACGAATTGTTCGTCAGACGTCTACAGCATTGAATAGACCTGCTGAGCTTTTCGTAAATAATACGGAAGGTGA
GTTGGACCGCAACATTTTAGAACGGTTAGTGACTCCGCTTGAGCATATGCTCAGAAACGCAATTGACCATGGTTTAGAAG
ACCGAGCGCAGCGGCAACAAGCAAATAAACCAGAAACAGGGCGTATTGAGCTTAATATTCAGCGTCAAGGTACGGATGTT
GTGGTTGTCTTTAGTGATGATGGTCAAGGTATTGATGTTGAAAAAGTACGTCAGAAAGCACTCCTTGCCGGATTGATCAA
GCCAGAACAAGACTTAGAACAGCAAGACATACTGCAACTCATTTTCCATCCGGGTTTAAGTACAGCAGAACAAGTCACTC
AGATTTCCGGACGTGGTGTAGGTCTTGATGTCGTACAGAGTGATATTAAGTCTTTGGGGGGACATGTCAGCGTTGAATCC
GTCTATGGGCAGGGAACAACATTTACGATTCGCGTACCGACTACAGTAGCGGTAAGTGATGCCTTAATGGTGAAAGTGGC
AGATCAACAATTTGCGATTCCACTCGCTCAAATCGATCGAATTATTCGAGTGTCACCAGCCTCTTTAGAGCAGTATTTTG
AAAGTCCTCAAGAACTATTTGAGTATGAAAATAAACGTTATCCATTACGTTATCTTTCAGAATTTGTTGGCAACCAACCC
ATACCACGTCTAAATGGCGTGATGTATTCATTACCTGTGTTAATGATTAAAGCCAATAATGGCCAAACTGTGGCACTACT
TGTTGACCAGTTAATTGGTTCTCGAGCGCAAATTGTAGTGAAACCAATTGGTCAACAGTTCTCTAGCATTGGGGCAATTG
CAGGCGCCACAATTTTAGGTGATGGACAGGTCTGCTTAATTTTAGATGGACAAAATATTGCGCGACAAATTCAGTCAACT
CAGCGTCATAAACAACTTAGTGAAGCGGTGTATAGACAACGAGAGTCTGATGAACGCCGCCTCATCATGATTGTAGATGA
CTCAGTAACTGTGCGTAAGGTCACCTCCCGATTACTTGAACGCCAAGGCTATGATGTGGTTACGGCTAAAGATGGTGTGG
ACGCCATAGAGCAGCTAGAAAATATTAAGCCAGATTTGATGCTACTTGATATTGAAATGCCACGTATGGATGGTTTTGAG
GTTCTCAATCTTGTACGACATCATGATATGCATCAATACATGCCAATTATTATGATTACATCCAGAACAGGTGAAAAACA
TCGTGAACGAGCATTCTCGTTAGGTGTGAGTCAATATATGGGTAAACCTTTCCAAGAAGAAGAGTTACTAGAGAATATTG
ATGCTTTACTTGTAGCGTTTGAAAGTGAGGTCAAATCATGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(1078-1214)

Hpt domain (Hpt)

(199-281)

Hpt domain (Hpt)

(732-796)

Hpt domain (Hpt)

(523-612)

Hpt domain (Hpt)

(25-106)

Response regulator receiver domain (Response_reg)

(1382-1493)

CheW-like domain (CheW)

(1221-1356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

39.655

95.868

0.38

Multiple sequence alignment    



References

[1] Nina Vesel et al. (2021) Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation. Journal of Bacteriology 203(8):e00034-21. [PMID: 33495250]