Detailed information    

experimental Experimentally validated

Overview

Name   pilG   Type   Regulator
Locus tag   H0N27_RS03110 Genome accession   NZ_CP059039
Coordinates   647649..648032 (+) Length   127 a.a.
NCBI ID   WP_000389061.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain A118     
Function   regulation of type IV pilus assembly   
Competence regulation

Function

In contrast to A. baylyi, deletion of pilS or pilR as well as chpA or pilG in A. baumannii completely abolished transformation, while the absence of pilH did not change the strain’s transformation efficiency


Genomic Context

Location: 642649..653032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0N27_RS03100 (H0N27_03100) - 645550..646668 (-) 1119 WP_001260821.1 efflux RND transporter periplasmic adaptor subunit -
  H0N27_RS03105 (H0N27_03105) - 646728..647366 (-) 639 WP_171945457.1 hypothetical protein -
  H0N27_RS03110 (H0N27_03110) pilG 647649..648032 (+) 384 WP_000389061.1 twitching motility response regulator PilG Regulator
  H0N27_RS03115 (H0N27_03115) - 648056..648418 (+) 363 WP_000101096.1 PleD family two-component system response regulator -
  H0N27_RS03120 (H0N27_03120) - 648479..649015 (+) 537 WP_000729762.1 chemotaxis protein CheW -
  H0N27_RS03125 (H0N27_03125) - 649062..651140 (+) 2079 WP_168726940.1 methyl-accepting chemotaxis protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  chpA pilG positive effect

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14200.23 Da        Isoelectric Point: 4.5762

>NTDB_id=1047 H0N27_RS03110 WP_000389061.1 647649..648032(+) (pilG) [Acinetobacter baumannii strain A118]
MEDAFQNLKVMVIDDSKTIRRTAETLLQREGCEVITAVDGFEALSKIAEANPDIVFVDIMMPRLDGYQTCALIKNSQNYQ
NIPVIMLSSKDGLFDQAKGRVVGSDEYLTKPFSKDELLNAIRNHVSS

Nucleotide


Download         Length: 384 bp        

>NTDB_id=1047 H0N27_RS03110 WP_000389061.1 647649..648032(+) (pilG) [Acinetobacter baumannii strain A118]
ATGGAAGATGCATTCCAAAATCTGAAAGTAATGGTTATTGATGACTCAAAAACTATACGCCGTACCGCAGAAACTCTATT
GCAACGCGAAGGTTGCGAAGTGATTACTGCTGTCGATGGATTTGAAGCTTTATCTAAAATTGCTGAAGCGAATCCGGATA
TTGTTTTTGTAGATATCATGATGCCTCGTTTAGACGGTTATCAAACTTGTGCTCTGATTAAGAACTCTCAAAATTATCAG
AACATTCCCGTTATCATGCTCTCTAGTAAAGATGGTTTATTTGATCAGGCAAAAGGGCGTGTGGTAGGTTCAGATGAATA
CTTGACGAAACCTTTTAGCAAAGATGAATTGCTAAATGCGATTCGTAATCATGTAAGTTCATAA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(10-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  vicR Streptococcus mutans UA159

42.735

92.126

0.394
  pilH Synechocystis sp. PCC 6803

41.593

93.388

0.388

Multiple sequence alignment    



References

[1] Nina Vesel et al. (2021) Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation. Journal of Bacteriology 203(8):e00034-21. [PMID: 33495250]