Detailed information NTG0001419

Experimentally validated

Overview

• Name: pilL-C
• Type: Machinery gene
• Locus tag: SGL_RS12550
• Genome accession: NC_000911
• Coordinates: 2271581..2274868 (+)
• Length: 1095 a.a.
• NCBI ID: WP_010873252.1
• Uniprot ID: -
• Organism: Synechocystis sp. PCC 6803
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

The pilJ and pilL-C mutants retained very slight competency (1.5% of wild type), although they showed non-motile phenotype and practically non-piliation in terms of the thick pilus structure.

Genomic Context

Location: 2266581..2279868

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SGL_RS12530 (sll0944)	-	2266850..2267344 (-)	495	WP_010873248.1	DUF1830 domain-containing protein	-
SGL_RS12535 (slr0320)	-	2267490..2269085 (+)	1596	WP_010873249.1	photosystem II high light acclimation radical SAM protein	-
SGL_RS12540 (sll0306)	-	2269126..2270163 (-)	1038	WP_010873250.1	RNA polymerase sigma factor, RpoD/SigA family	-
SGL_RS12545 (slr0321)	era	2270624..2271571 (+)	948	WP_010873251.1	GTPase Era	-
SGL_RS12550 (slr0322)	pilL-C	2271581..2274868 (+)	3288	WP_010873252.1	hybrid sensor histidine kinase/response regulator	Machinery gene
SGL_RS12555 (slr0323)	-	2274868..2277996 (+)	3129	WP_010873253.1	alpha-mannosidase	-
SGL_RS12560 (slr0324)	-	2277989..2278873 (+)	885	WP_010873254.1	ABC transporter permease	-
SGL_RS12565 (slr0325)	-	2278921..2279406 (+)	486	WP_010873255.1	MEKHLA domain-containing protein	-

Sequence

Protein

• Length: 1095 a.a.
• Molecular weight: 120557.37 Da
• Isoelectric Point: 4.3786

>NTDB_id=1419 SGL_RS12550 WP_010873252.1 2271581..2274868(+) (pilL-C) [Synechocystis sp. PCC 6803]
MTSDPNPAQAFENWFEEEEGDDAFTSLIAGEETNSGIEELDFGDLFEGEPTVIQGEGFGSPSSGEEDPPVRMTLDFNSPE
AELDALFTDEDAPTTAIDPFESSSWLEIDQMMEADPLASIPETNAPAYQTAGGLDDNDLAELQDLLGVGDVEPAITNPGL
HSFAGADRVTPLTNEVLLFAAIDDLDGLINAPVLDEEFSFTELETLIDTPAPKSFATISHPFRLPVAMEEDEDEFKDLEA
LLEQADSSLGGGIAGMGGARLPARPRQVKSRSFEQTMRVPIKQLDNLSNLIGELVVKRNRLEQDQERLRQFLDNLLNQAQ
NLSDVGGRMQDLYERTLLEGALLASRSTNSRHHSSKTTYPDARITASSSSTMNDQPLDALEMDRFTGFHLLSQEMIELIV
RVRESASDIQYLVDETDQVARTLRQVTTQLQEGMTKSRMVPFTQTADRLPRAIREVSMKLNKKVNLEVEGGDVLIDKMIL
EHLYNPMTHLVNNAITHGIETPAERQKLGKSPEGHLAVRAFLQGNQTLITVSDDGAGINTEMVKQKAVEKGLISRMEAQS
LSQSEVYDLLFHAGFSTKDTADDFAGRGVGLDVVRTSLIDIRGAINIESTVGKGTTFTIRLPLTLSICKALCCLSDRARI
AFPMDGVEDTRDYLPKDIVTDEDGQKCVKYGKEALLLPLYPLKDLLNYNRQLSRGSVYGGKQEEDVVRVIIARGSSGNLL
ALQIDQVLGEEEIVIKQIEGPIPKPPGIAGATVRGDGSIMPIADVLELIEIAQGRLRTDSSGGLWRKTLTPTVNEVHMDP
TVLIVDDSITVRELLSLSFKKAGFRVEQARDGQEAWDQLKSGLPCDLVFCDIEMPRKNGLELLSELQADEELSSIPVALL
TSRGADRHRKVAAKLGATGYFTKPYTEKDILDAANRMLNGEVLLPGSTRKTNSILSRFKKARGNFSEEAPTIVRSNPGTL
RSTVLIIDDSVMVREMLSMTFKKADYHTETARDGQDAWEKLKSGLPCDLILCDIEMPRMNGLELLERIQEDPNLSRIPVA
MITSRGAQKMQRMAAEKGAKGYFVKPYIEETLLEASQRLIRGEVLLDLAALSADE

Nucleotide

• Length: 3288 bp

>NTDB_id=1419 SGL_RS12550 WP_010873252.1 2271581..2274868(+) (pilL-C) [Synechocystis sp. PCC 6803]
ATGACTAGCGATCCCAATCCAGCCCAGGCATTTGAAAATTGGTTTGAGGAGGAAGAGGGGGATGACGCCTTTACTTCCCT
CATTGCCGGTGAGGAGACTAACAGTGGGATTGAAGAACTGGATTTTGGCGATTTATTTGAGGGAGAACCCACCGTCATCC
AGGGTGAAGGGTTTGGCTCGCCGTCCTCTGGAGAGGAAGATCCGCCGGTCCGCATGACCCTAGATTTTAATTCCCCGGAA
GCGGAACTAGACGCTCTCTTTACCGATGAGGATGCCCCCACAACGGCGATCGATCCCTTTGAAAGCTCCAGTTGGCTAGA
AATCGACCAGATGATGGAAGCTGATCCCCTGGCTTCCATTCCTGAGACCAATGCCCCCGCTTACCAGACCGCTGGTGGCC
TAGATGACAATGACCTAGCCGAATTGCAGGATTTACTAGGAGTAGGGGACGTCGAGCCCGCCATTACCAACCCTGGTTTG
CACTCCTTTGCTGGGGCTGATCGGGTAACTCCATTGACAAATGAGGTCCTGCTTTTTGCGGCGATCGATGACTTAGACGG
ATTGATTAATGCCCCTGTCCTGGACGAAGAATTTTCCTTTACCGAACTAGAGACTTTAATCGATACCCCAGCCCCAAAAT
CTTTTGCCACCATCAGCCATCCTTTTCGTTTACCGGTGGCTATGGAGGAAGACGAGGATGAATTCAAAGACTTAGAAGCA
TTATTAGAGCAAGCGGATAGCAGTCTGGGGGGGGGCATAGCCGGCATGGGAGGGGCTCGGCTGCCCGCTCGACCTAGGCA
GGTTAAATCCCGTAGTTTTGAGCAAACTATGCGGGTGCCCATCAAACAACTTGACAACCTCAGTAACCTGATCGGGGAGC
TAGTGGTCAAACGGAATCGCCTTGAACAGGATCAGGAGCGTTTACGACAGTTTTTGGATAATCTGCTCAACCAAGCCCAA
AACCTCAGCGATGTGGGGGGGCGGATGCAGGATTTATACGAAAGAACTTTGTTGGAAGGGGCCCTTTTAGCCAGCCGCAG
CACCAACAGCCGTCACCACTCCTCGAAAACCACCTACCCCGATGCCAGGATCACGGCCTCTAGTTCCTCGACCATGAATG
ACCAGCCTTTGGATGCGCTGGAAATGGATCGATTTACCGGATTTCACCTGCTTTCCCAGGAGATGATCGAGTTAATTGTG
CGGGTGCGGGAATCTGCGTCGGATATTCAGTACTTGGTGGACGAGACAGATCAGGTGGCCCGAACCCTCCGGCAGGTGAC
TACGCAGTTACAGGAGGGAATGACCAAATCTCGCATGGTGCCCTTTACCCAAACCGCCGATCGCCTGCCACGGGCCATCC
GGGAAGTGTCCATGAAATTGAATAAAAAAGTCAACCTAGAGGTGGAAGGGGGCGACGTGCTCATCGACAAAATGATTTTG
GAGCACCTCTACAATCCCATGACCCATTTGGTTAACAATGCCATCACCCACGGTATTGAAACCCCAGCAGAACGACAAAA
ATTAGGCAAATCCCCCGAAGGGCATCTAGCAGTGCGGGCCTTTCTCCAGGGCAACCAAACCCTGATCACCGTCTCCGATG
ATGGAGCAGGGATCAACACGGAAATGGTTAAACAAAAAGCAGTGGAAAAAGGGCTCATTAGCCGTATGGAGGCCCAGAGC
CTATCCCAGTCAGAGGTTTATGACCTCCTGTTCCATGCTGGTTTTAGCACCAAGGACACAGCGGACGACTTTGCCGGTAG
GGGAGTAGGTCTTGACGTGGTACGCACCAGTCTGATCGATATTCGCGGAGCCATCAACATTGAATCCACTGTGGGCAAGG
GCACCACTTTTACCATTCGTTTGCCCCTCACCCTGAGCATTTGCAAAGCCCTTTGTTGCCTGAGCGATCGGGCTCGCATC
GCCTTTCCCATGGACGGGGTGGAAGATACCAGGGACTATTTGCCCAAAGACATTGTTACCGATGAGGATGGTCAAAAGTG
CGTTAAATACGGCAAGGAAGCCCTGCTTTTACCGCTCTATCCCCTCAAAGATTTGCTCAACTATAACCGGCAATTAAGCC
GGGGTAGTGTGTATGGGGGCAAGCAAGAGGAAGATGTGGTGCGGGTCATCATTGCCCGGGGGAGTTCTGGTAACCTGTTA
GCCCTCCAGATAGACCAGGTATTAGGGGAAGAAGAAATTGTTATCAAGCAAATTGAAGGGCCCATTCCCAAACCTCCAGG
CATTGCCGGGGCTACCGTGCGGGGGGATGGTAGCATCATGCCCATTGCCGATGTTTTGGAACTGATCGAAATTGCCCAGG
GTCGTCTCCGCACTGACAGCAGTGGTGGACTATGGCGCAAAACCCTGACCCCCACGGTCAATGAAGTCCACATGGACCCC
ACTGTGCTAATTGTGGACGACTCCATCACGGTGCGGGAATTGCTCTCCCTCAGTTTCAAAAAAGCTGGTTTCCGGGTAGA
ACAGGCTAGGGATGGACAGGAAGCCTGGGATCAACTCAAGTCTGGTCTGCCCTGTGACTTGGTCTTCTGTGACATTGAAA
TGCCCCGCAAAAATGGCTTGGAATTGTTATCGGAACTGCAAGCAGACGAAGAACTATCTTCCATTCCAGTGGCCTTACTC
ACTTCCAGGGGAGCCGATCGCCATCGTAAAGTGGCGGCCAAGCTGGGGGCAACGGGCTATTTCACCAAGCCCTATACAGA
AAAAGACATTTTGGACGCGGCCAACCGGATGTTGAATGGGGAGGTGTTACTCCCCGGCAGTACCCGCAAAACTAATTCCA
TTCTTTCTCGCTTCAAAAAAGCCCGAGGCAACTTCTCCGAAGAAGCTCCCACCATTGTGAGAAGTAATCCAGGTACCCTA
CGTTCGACGGTGTTAATTATTGATGACTCGGTGATGGTGCGGGAAATGCTTTCTATGACCTTTAAAAAAGCCGATTACCA
TACAGAAACTGCTCGGGATGGCCAGGATGCCTGGGAAAAACTCAAGTCTGGCTTACCCTGTGATTTGATTCTCTGTGACA
TTGAAATGCCCCGCATGAACGGTCTGGAATTATTGGAGCGCATTCAAGAAGATCCCAACCTTTCCCGTATTCCAGTGGCC
ATGATTACCTCCCGGGGAGCACAAAAAATGCAGCGCATGGCAGCAGAAAAAGGCGCCAAGGGATACTTTGTGAAGCCCTA
CATTGAGGAAACTTTATTGGAAGCCAGTCAAAGGTTGATAAGGGGAGAAGTGTTGCTAGATTTGGCCGCTCTAAGTGCAG
ACGAGTAA

Domains

Predicted by InterproScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (487-624)

• Signal transducing histidine kinase, homodimeric domain (H-kinase_dim) (275-331)

• CheW-like domain (CheW) (630-770)

• Response regulator receiver domain (Response_reg) (964-1075)

• Response regulator receiver domain (Response_reg) (802-914)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilH	Synechocystis sp. PCC 6803	37.5	99.174	0.372

References

• [1]: Shizue Yoshihara et al. (2002) pilG Gene cluster and split pilL genes involved in pilus biogenesis, motility and genetic transformation in the cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology 43(5):513-21. [PMID: 12040098]