Detailed information    

experimental Experimentally validated

Overview

Name   covR   Type   Regulator
Locus tag   HSISS4_RS01430 Genome accession   NZ_CP013216
Coordinates   276414..277103 (+) Length   229 a.a.
NCBI ID   WP_002883757.1    Uniprot ID   A0AB33ZE44
Organism   Streptococcus salivarius strain HSISS4     
Function   repress comR expression   
Competence regulation

Function

CovRS, a well-described virulence regulatory system in pathogenic streptococci, directly controls the ComRS system to generate bimodality in the gut commensal Streptococcus salivarius and the closely related species Streptococcus thermophilus. CovRS is a direct repression system of comR expression.


Genomic Context

Location: 271414..282103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSISS4_RS01405 (HSISS4_00241) - 271646..272476 (+) 831 WP_021143559.1 energy-coupling factor transporter transmembrane component T family protein -
  HSISS4_RS01410 (HSISS4_00242) - 272566..273237 (-) 672 WP_021143560.1 potassium channel family protein -
  HSISS4_RS01415 (HSISS4_00243) - 273277..274641 (-) 1365 WP_081051370.1 TrkH family potassium uptake protein -
  HSISS4_RS01420 (HSISS4_00244) rsmG 274710..275423 (-) 714 WP_013990060.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  HSISS4_RS01425 (HSISS4_00245) - 275722..276258 (+) 537 WP_002883813.1 DUF177 domain-containing protein -
  HSISS4_RS01430 (HSISS4_00246) covR 276414..277103 (+) 690 WP_002883757.1 response regulator transcription factor Regulator
  HSISS4_RS01435 (HSISS4_00247) covS 277100..278629 (+) 1530 WP_002883784.1 HAMP domain-containing sensor histidine kinase Regulator
  HSISS4_RS01440 (HSISS4_00248) nrdR 278751..279230 (+) 480 WP_002883820.1 transcriptional regulator NrdR -
  HSISS4_RS01445 (HSISS4_00249) - 279227..280402 (+) 1176 WP_021143563.1 DnaD domain protein -
  HSISS4_RS01450 (HSISS4_00250) dnaI 280406..281308 (+) 903 WP_002883792.1 primosomal protein DnaI -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  covR comR negative effect
  comR comX/sigX positive effect
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect
  stkP/pknB comX/sigX positive effect
  comS comX/sigX positive effect
  covS comR negative effect
  pptA comS positive effect
  amiD comS positive effect
  pptB comS positive effect
  amiE comS positive effect
  amiC comS positive effect
  amiF comS positive effect
  pepF comS negative effect
  amiA comS positive effect

Sequence

Protein


Download         Length: 229 a.a.        Molecular weight: 26501.51 Da        Isoelectric Point: 4.6354

>NTDB_id=471 HSISS4_RS01430 WP_002883757.1 276414..277103(+) (covR) [Streptococcus salivarius strain HSISS4]
MSKRILIVEDEKNLARFVSLELQHEGYDVVTADNGREGLEMALEKDFDLILLDLMLPEMDGFEVTRRLQQEKDTYIMMMT
ARDSIMDIVAGLDRGADDYIVKPFAIEELLARIRATFRRQDIEAAKNAPAKASTYRDLKLDVQNRTVVRGDEAIPLTKRE
FDLLNTLLSNMNQVMTREELLLQVWKYDDAIETNVVDVYIRYLRGKIDVPGKESYIQTVRGMGYVIREK

Nucleotide


Download         Length: 690 bp        

>NTDB_id=471 HSISS4_RS01430 WP_002883757.1 276414..277103(+) (covR) [Streptococcus salivarius strain HSISS4]
ATGAGCAAACGCATTTTGATTGTTGAAGATGAGAAAAACCTTGCTCGTTTTGTCTCTCTTGAACTTCAACACGAAGGATA
CGACGTTGTGACAGCAGACAACGGACGCGAAGGACTTGAAATGGCACTCGAGAAAGATTTCGATCTTATTCTCTTGGACC
TTATGTTGCCTGAGATGGACGGTTTCGAAGTAACACGTCGTCTCCAACAAGAAAAAGATACTTATATTATGATGATGACA
GCTCGCGATTCAATCATGGACATTGTTGCTGGTTTGGACCGCGGTGCTGATGACTATATTGTAAAACCATTTGCGATTGA
AGAATTGTTGGCACGTATCCGTGCAACCTTCCGTCGCCAAGATATCGAAGCTGCTAAGAATGCACCAGCAAAAGCATCAA
CTTATCGTGATTTGAAATTGGATGTTCAAAACCGTACAGTTGTTCGTGGTGATGAAGCAATTCCATTGACAAAACGTGAG
TTTGATTTGTTGAACACACTTTTGAGCAATATGAACCAAGTGATGACCCGTGAGGAACTCTTGCTTCAAGTTTGGAAATA
TGATGACGCCATCGAAACAAACGTGGTAGATGTTTATATTCGTTACTTGCGTGGAAAAATCGACGTTCCAGGTAAGGAAT
CATATATTCAAACAGTACGAGGAATGGGTTACGTTATCCGTGAAAAATGA

Domains

Predicted by InterproScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(152-225)

Response regulator receiver domain (Response_reg)

(5-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  covR Lactococcus lactis subsp. lactis strain DGCC12653

68.421

99.563

0.681
  vicR Streptococcus mutans UA159

45.923

100

0.467
  micA Streptococcus pneumoniae Cp1015

44.156

100

0.445

Multiple sequence alignment    



References

[1] Adrien Knoops et al. (2022) The CovRS Environmental Sensor Directly Controls the ComRS Signaling System To Orchestrate Competence Bimodality in Salivarius Streptococci. MBio 13(1):e0312521. [PMID: 35089064]