Detailed information    

experimental Experimentally validated

Overview

Name   amiC   Type   Regulator
Locus tag   HSISS4_RS06995 Genome accession   NZ_CP013216
Coordinates   1559591..1561084 (-) Length   497 a.a.
NCBI ID   WP_013990225.1    Uniprot ID   A0AAW6DBW1
Organism   Streptococcus salivarius strain HSISS4     
Function   internalize XIP   
Competence regulation

Function

We have shown the necessity for a pheromone exporter (PptAB) and a peptide importer (Ami/Opp) for competence activation through ComR overproduction.


Genomic Context

Location: 1554591..1566084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSISS4_RS06965 (HSISS4_01358) - 1554719..1555096 (+) 378 WP_021143815.1 GntR family transcriptional regulator -
  HSISS4_RS06970 (HSISS4_01359) - 1555101..1555799 (+) 699 WP_021143814.1 ABC transporter ATP-binding protein -
  HSISS4_RS06975 (HSISS4_01360) - 1555811..1556596 (+) 786 WP_021143813.1 hypothetical protein -
  HSISS4_RS06980 (HSISS4_01361) amiF 1556648..1557577 (-) 930 WP_021143812.1 ATP-binding cassette domain-containing protein Regulator
  HSISS4_RS06985 (HSISS4_01362) amiE 1557570..1558655 (-) 1086 WP_021143811.1 ABC transporter ATP-binding protein Regulator
  HSISS4_RS06990 (HSISS4_01363) amiD 1558665..1559591 (-) 927 WP_021143810.1 oligopeptide ABC transporter permease OppC Regulator
  HSISS4_RS06995 (HSISS4_01364) amiC 1559591..1561084 (-) 1494 WP_013990225.1 ABC transporter permease Regulator
  HSISS4_RS07000 (HSISS4_01365) amiA 1561145..1563115 (-) 1971 WP_013990224.1 peptide ABC transporter substrate-binding protein Regulator
  HSISS4_RS07005 (HSISS4_01366) amiA 1563297..1565267 (-) 1971 WP_021143809.1 peptide ABC transporter substrate-binding protein Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  amiC comS positive effect
  comS comX/sigX positive effect
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect
  stkP/pknB comX/sigX positive effect
  comR comX/sigX positive effect
  covS comR negative effect
  covR comR negative effect
  pptA comS positive effect
  amiD comS positive effect
  pptB comS positive effect
  amiE comS positive effect
  amiF comS positive effect
  pepF comS negative effect
  amiA comS positive effect

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55920.66 Da        Isoelectric Point: 9.9550

>NTDB_id=476 HSISS4_RS06995 WP_013990225.1 1559591..1561084(-) (amiC) [Streptococcus salivarius strain HSISS4]
MKKYILLRTLRSLLSIFVVTALTYTIIYTMVPRRLIFRQDPNYNKIAKTADSKANYENTIYERMGYIDYYDTKELQEKAS
SLDKSVTTKPTKENKAIYQKYVDSLGHGWRLHQFKQSKQFYATRDVPVLERVFGFYTHLFEFDNTGWVKDKTNPNLKRYI
RIENDPAIGWSVVGSGTKHKYLLYFNSQFPFVHQNFVKMNLGTSYPTYAEQGVLDVITQGQGQTQSSEVNFPTGKKVSSV
DIYSRTYKSPSQADARDRSYYGDDPYTATKSRYQYPSMVTSSAIAGLIGLVLSYALAIPLGSYMARFKNTLFDSVSTGIL
TFLLSLPTIALVYIIRLIGSEIGLPDSFPILGAGDWRSYVLPSVILGLLSTPGLAIWIRRYMIDLQSQDFVRFARAKGLS
EQEISNKHIFKNAMVSLVSGIPASIVSVITGATLTETIFAYPGMGKMLIDSVRASNNAMVVGLVFIFTALTIFSLLVGDI
LMTMIDPRIKLTSKGGK

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=476 HSISS4_RS06995 WP_013990225.1 1559591..1561084(-) (amiC) [Streptococcus salivarius strain HSISS4]
ATGAAAAAATACATTTTATTGAGAACCCTCCGTTCTCTATTGTCAATATTTGTCGTGACGGCCTTGACCTACACGATTAT
CTATACCATGGTGCCGAGGCGTCTGATTTTCAGACAGGACCCTAACTACAACAAGATTGCTAAGACAGCTGATTCTAAGG
CTAACTATGAAAATACAATCTATGAACGTATGGGTTACATTGATTACTATGACACCAAGGAGTTGCAGGAGAAAGCTAGC
TCCCTTGATAAGTCAGTAACCACTAAGCCAACAAAAGAAAATAAAGCTATTTACCAAAAATATGTGGATAGTTTAGGACA
TGGCTGGCGTTTGCATCAATTCAAGCAAAGTAAACAATTCTATGCAACACGTGATGTTCCAGTGCTTGAACGTGTATTTG
GCTTCTATACGCACCTCTTTGAATTTGACAATACTGGATGGGTTAAAGATAAAACAAATCCAAACTTGAAGCGTTACATC
CGTATTGAAAATGACCCAGCTATCGGTTGGTCAGTTGTCGGATCAGGGACTAAGCATAAATACTTGCTTTACTTTAACAG
TCAGTTCCCATTTGTTCACCAAAACTTTGTGAAGATGAACCTTGGTACATCTTACCCAACCTATGCTGAGCAGGGTGTTC
TTGATGTTATTACACAAGGACAAGGGCAAACACAAAGCTCTGAGGTCAACTTCCCTACTGGTAAGAAAGTATCTTCAGTA
GATATCTACTCTCGTACTTATAAATCACCAAGTCAGGCTGATGCTAGGGATCGTTCTTACTATGGTGACGACCCTTACAC
AGCTACTAAGAGTCGCTACCAGTACCCATCAATGGTGACAAGTTCTGCCATTGCTGGTTTGATTGGTTTGGTCTTGTCTT
ATGCTTTGGCAATTCCATTGGGATCATACATGGCACGCTTTAAAAACACACTCTTTGATAGTGTGTCAACTGGTATCTTG
ACTTTCTTGCTTTCACTACCAACCATCGCCTTGGTTTATATCATTCGTTTGATTGGTTCTGAGATTGGTCTTCCAGATTC
TTTCCCTATCTTGGGTGCGGGTGACTGGCGTTCATACGTCTTGCCATCTGTAATTCTCGGTCTCTTGTCTACACCAGGTC
TTGCCATTTGGATTCGTCGTTACATGATTGACTTGCAGTCACAAGACTTCGTTCGTTTTGCACGTGCCAAAGGGTTGTCA
GAACAAGAAATTTCGAATAAACACATTTTCAAAAATGCTATGGTTTCCTTGGTTTCAGGGATCCCAGCATCTATCGTTAG
TGTTATCACAGGGGCAACCTTGACAGAAACAATCTTTGCCTACCCTGGTATGGGTAAAATGTTGATTGACTCTGTCCGTG
CTTCAAATAACGCTATGGTTGTGGGTCTTGTATTTATTTTCACAGCCCTCACCATCTTCTCATTGCTTGTGGGTGATATC
CTTATGACTATGATTGACCCACGTATCAAATTGACATCTAAAGGAGGTAAATAA

Domains

Predicted by InterproScan.

Binding-protein-dependent transport system inner membrane component (BPD_transp_1)

(297-490)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiC Streptococcus thermophilus LMG 18311

97.384

100

0.974
  amiC Streptococcus thermophilus LMD-9

96.982

100

0.97

Multiple sequence alignment    



References

[1] Adrien Knoops et al. (2022) The CovRS Environmental Sensor Directly Controls the ComRS Signaling System To Orchestrate Competence Bimodality in Salivarius Streptococci. MBio 13(1):e0312521. [PMID: 35089064]