Detailed information    

experimental Experimentally validated

Overview

Name   amiC   Type   Regulator
Locus tag   STER_RS06925 Genome accession   NC_008532
Coordinates   1316081..1317574 (-) Length   497 a.a.
NCBI ID   WP_011681416.1    Uniprot ID   -
Organism   Streptococcus thermophilus LMD-9     
Function   internalize XIP   
Competence regulation

Function

XIP peptides are recognized by the oligopeptide-binding protein AmiA3 and internalized by the oligopeptide Ami transporter (AmiCDEF).


Genomic Context

Location: 1311081..1322574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STER_RS06895 (STER_1402) - 1311207..1311584 (+) 378 WP_011681413.1 GntR family transcriptional regulator -
  STER_RS06900 (STER_1403) - 1311589..1312287 (+) 699 WP_011226301.1 ABC transporter ATP-binding protein -
  STER_RS06905 (STER_1404) - 1312299..1313084 (+) 786 WP_011681414.1 hypothetical protein -
  STER_RS06910 (STER_1405) amiF 1313138..1314067 (-) 930 WP_011681415.1 ATP-binding cassette domain-containing protein Regulator
  STER_RS06915 (STER_1406) amiE 1314060..1315145 (-) 1086 WP_011226304.1 ABC transporter ATP-binding protein Regulator
  STER_RS06920 (STER_1407) amiD 1315155..1316081 (-) 927 WP_002946409.1 oligopeptide ABC transporter permease OppC Regulator
  STER_RS06925 (STER_1408) amiC 1316081..1317574 (-) 1494 WP_011681416.1 ABC transporter permease Regulator
  STER_RS06930 (STER_1409) amiA 1317636..1319603 (-) 1968 WP_011681417.1 peptide ABC transporter substrate-binding protein Regulator
  STER_RS06935 (STER_1410) - 1319959..1321136 (+) 1178 Protein_1298 ISL3 family transposase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  amiC comS positive effect
  comS comX positive effect
  comX late competence genes positive effect
  comX late competence genes positive effect
  comR comX positive effect
  clpP comX negative effect
  mecA comX negative effect
  clpC comX negative effect
  comR comS positive effect
  comS comS positive effect
  amiD comS positive effect
  pptA comS positive effect
  amiE comS positive effect
  eeP comS positive effect
  amiA3 comS positive effect
  pptB comS positive effect
  amiF comS positive effect

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55635.50 Da        Isoelectric Point: 10.0972

>NTDB_id=293 STER_RS06925 WP_011681416.1 1316081..1317574(-) (amiC) [Streptococcus thermophilus LMD-9]
MKKYILLRTLRSLLSIFVVTALTYTIIYTMVPRRLIFRQDPNYNKIAKTADSKANYENTIYERLGYIDYYDTKELQEKAS
SLDKSVTTKPTKENKAVYQKYVDSLGRGWRLHQFKQSKQFYATRDVPVLERVFGFYTHLFEFDNTGWVKDKTNPNLKRYI
RIENDPAIGWSVVGSGTKHKYLLYFNGQFPFVHQNFVKMNLGTSYPTYAGQGVLDVITQGQGQTKSSVVNFPTGKKISSV
DIYSRTYKSPSQADARDRSYYGNDPYTATKSRYQYPSMVTSSAIAGLIGLVLSYALAIPLGSYMALFKNTLFDSVSTGVL
TFLLSLPTIALVYIIRLIGSEIGLPDSFPILGAGDWRSYVLPSVILGLLSTPGLAIWIRRYMIDLQSQDFVRFARAKGLS
EQEISNKHILKNAMVSLVSGIPASIVGVITGATLTETIFAYPGMGKMLIDSVRASNNAMVVGLVFIFTALSIFSLLVGDI
LMTIIDPRIKLTSKGGK

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=293 STER_RS06925 WP_011681416.1 1316081..1317574(-) (amiC) [Streptococcus thermophilus LMD-9]
ATGAAAAAATACATTTTATTGAGAACACTCCGTTCTCTATTGTCAATATTTGTCGTGACGGCTTTGACCTACACGATTAT
CTATACCATGGTGCCGAGGCGTCTGATTTTCAGACAAGACCCGAACTACAACAAGATTGCTAAGACAGCTGATTCTAAGG
CTAACTATGAAAATACAATCTATGAACGCTTGGGTTACATTGATTACTATGACACCAAGGAGTTGCAGGAGAAAGCTAGC
TCCCTTGATAAGTCAGTAACCACTAAGCCAACAAAAGAAAATAAGGCTGTCTACCAAAAATATGTGGATAGTTTAGGACG
TGGTTGGCGTTTGCATCAGTTCAAGCAAAGTAAACAATTCTATGCGACACGTGATGTGCCAGTGCTTGAACGTGTGTTTG
GCTTCTATACGCACCTCTTTGAATTTGACAATACTGGATGGGTTAAAGATAAAACAAATCCAAACTTGAAGCGTTATATC
CGTATTGAAAATGACCCAGCTATCGGTTGGTCAGTTGTTGGTTCAGGGACTAAGCATAAATATTTGCTTTACTTTAATGG
TCAGTTCCCATTTGTTCACCAAAATTTTGTGAAGATGAATCTTGGAACTTCATACCCAACTTATGCTGGGCAAGGTGTTC
TTGATGTTATTACACAAGGCCAAGGACAGACAAAAAGCTCTGTGGTTAACTTCCCTACTGGTAAGAAAATATCTTCAGTA
GATATCTACTCTCGTACTTATAAATCACCAAGTCAGGCTGACGCTAGAGATCGTTCTTACTATGGAAATGATCCATACAC
AGCTACCAAGAGTCGTTATCAATACCCATCAATGGTGACAAGTTCTGCCATTGCAGGTTTGATTGGTTTGGTCTTGTCTT
ATGCTTTGGCAATTCCATTGGGATCATACATGGCACTCTTTAAAAACACACTCTTTGATAGTGTGTCAACAGGTGTCTTG
ACTTTCTTGCTTTCACTACCAACCATCGCCTTGGTTTATATCATTCGTTTGATTGGTTCTGAGATTGGTCTTCCAGATTC
CTTCCCTATCTTGGGTGCAGGTGACTGGCGTTCATACGTCTTGCCATCTGTTATTCTCGGTCTCTTGTCAACACCAGGTC
TTGCGATTTGGATTCGTCGTTACATGATTGACTTGCAATCACAAGACTTCGTTCGTTTTGCACGTGCCAAAGGGTTGTCA
GAACAAGAAATTTCAAATAAACACATTTTAAAAAACGCTATGGTTTCCTTGGTTTCAGGGATCCCAGCATCTATTGTTGG
GGTTATCACAGGGGCAACCTTGACAGAAACAATCTTCGCCTACCCTGGTATGGGTAAAATGTTGATCGATTCTGTTCGTG
CTTCTAATAATGCTATGGTCGTTGGGCTGGTATTTATTTTTACAGCTCTCTCCATCTTCTCATTGCTTGTGGGTGATATC
CTTATGACTATTATTGACCCACGTATCAAATTGACATCTAAAGGAGGTAAATAA

Domains

Predicted by InterproScan.

Binding-protein-dependent transport system inner membrane component (BPD_transp_1)

(297-490)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiC Streptococcus thermophilus LMG 18311

99.195

100

0.992
  amiC Streptococcus salivarius strain HSISS4

96.982

100

0.97

Multiple sequence alignment    



References

[1] Rozenn Gardan et al. (2013) Extracellular life cycle of ComS, the competence-stimulating peptide of Streptococcus thermophilus. Journal of Bacteriology 195(8):1845-55. [PMID: 23396911]
[2] Laetitia Fontaine et al. (2010) A novel pheromone quorum-sensing system controls the development of natural competence in Streptococcus thermophilus and Streptococcus salivarius. Journal of Bacteriology 192(5):1444-54. [PMID: 20023010]
[3] Rozenn Gardan et al. (2009) The oligopeptide transport system is essential for the development of natural competence in Streptococcus thermophilus strain LMD-9. Journal of Bacteriology 191(14):4647-55. [PMID: 19447907]