Detailed information    

experimental Experimentally validated

Overview

Name   mecA   Type   Regulator
Locus tag   STER_RS01050 Genome accession   NC_008532
Coordinates   182275..183024 (+) Length   249 a.a.
NCBI ID   WP_011680667.1    Uniprot ID   Q03MN5
Organism   Streptococcus thermophilus LMD-9     
Function   degradation of ComX   
Competence regulation

Function

σX degradation is performed by the MecA–ClpCP complex.


Genomic Context

Location: 177275..188024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STER_RS11165 (STER_0211) - 177851..178021 (+) 171 WP_011680664.1 hypothetical protein -
  STER_RS09970 (STER_0212) - 178108..178215 (+) 108 WP_011226802.1 LPXTG cell wall anchor domain-containing protein -
  STER_RS01035 (STER_0213) - 178302..179042 (-) 741 WP_002949253.1 amino acid ABC transporter ATP-binding protein -
  STER_RS01040 (STER_0214) - 179042..180592 (-) 1551 WP_011680665.1 ABC transporter substrate-binding protein/permease -
  STER_RS01045 (STER_0215) - 180795..181649 (+) 855 WP_011680666.1 undecaprenyl-diphosphate phosphatase -
  STER_RS09975 - 181701..182009 (-) 309 WP_306496352.1 DUF3021 family protein -
  STER_RS01050 (STER_0216) mecA 182275..183024 (+) 750 WP_011680667.1 adaptor protein MecA Regulator
  STER_RS01055 (STER_0217) - 183028..184185 (+) 1158 WP_011680668.1 glycosyltransferase family 4 protein -
  STER_RS01060 (STER_0218) sufC 184346..185116 (+) 771 WP_002947634.1 Fe-S cluster assembly ATPase SufC -
  STER_RS01065 (STER_0219) sufD 185153..186415 (+) 1263 WP_011680669.1 Fe-S cluster assembly protein SufD -
  STER_RS01070 (STER_0220) - 186469..187701 (+) 1233 WP_011680670.1 cysteine desulfurase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  mecA comX negative effect
  comX late competence genes positive effect
  comX late competence genes positive effect
  comR comX positive effect
  comR comS positive effect
  comS comX positive effect
  comS comS positive effect
  amiD comS positive effect
  pptA comS positive effect
  amiE comS positive effect
  eeP comS positive effect
  amiA3 comS positive effect
  pptB comS positive effect
  amiF comS positive effect
  amiC comS positive effect
  clpP comX negative effect
  clpC comX negative effect

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 29174.68 Da        Isoelectric Point: 4.0481

>NTDB_id=299 STER_RS01050 WP_011680667.1 182275..183024(+) (mecA) [Streptococcus thermophilus LMD-9]
MEMKQISETTLKIMITMEDLEEHGMELKDFLIPQEKTEEFFYTVMDELDLPDNFKNSGMLSFRVTPRKDRVDVFVTKSDL
KEDLDFNDLSDMEDYSGLSPEEFLKALEDNFMDKGDIEAHHKLEEHDKTLKEVDETMTEPAKEVAEETIREDYTHYVLAF
SDFDQVVTFAQGLKNVSVEGSEFYKLGDVYYMTILLYLADEPDYYANNMYARFLEYANVADRTRPYLQEHATILMEEDAL
PVLQATKWS

Nucleotide


Download         Length: 750 bp        

>NTDB_id=299 STER_RS01050 WP_011680667.1 182275..183024(+) (mecA) [Streptococcus thermophilus LMD-9]
ATGGAAATGAAACAAATAAGCGAGACAACTCTAAAAATCATGATTACCATGGAAGATCTTGAAGAACATGGTATGGAGCT
GAAAGATTTCTTGATTCCCCAGGAAAAAACAGAGGAATTTTTCTATACTGTCATGGATGAGTTGGACTTGCCTGATAACT
TTAAAAATAGTGGGATGTTGAGCTTCCGTGTCACGCCACGAAAGGATCGTGTGGATGTTTTTGTGACTAAATCAGATCTT
AAGGAAGACCTTGATTTCAATGATTTGTCAGATATGGAAGACTATTCAGGACTTTCTCCAGAAGAATTCCTCAAAGCTCT
TGAAGATAACTTTATGGATAAGGGAGATATTGAAGCCCACCATAAGCTCGAAGAGCATGATAAGACCTTGAAAGAGGTTG
ATGAGACTATGACAGAGCCGGCTAAGGAAGTGGCTGAGGAAACTATTCGTGAAGATTATACTCACTATGTCTTGGCTTTC
TCTGACTTTGATCAAGTAGTGACCTTTGCGCAAGGTTTGAAGAATGTGTCAGTGGAGGGTTCTGAATTTTATAAACTTGG
TGATGTCTACTATATGACTATCCTTCTTTACTTGGCTGATGAGCCTGATTATTACGCGAACAATATGTATGCGCGTTTTC
TTGAATATGCGAATGTGGCAGACCGAACACGTCCCTATCTACAAGAGCATGCAACAATCTTGATGGAAGAGGACGCCTTA
CCGGTATTACAAGCAACGAAATGGAGCTAG

Domains

Predicted by InterproScan.

Negative regulator of genetic competence (MecA) (MecA)

(1-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q03MN5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mecA Streptococcus thermophilus LMG 18311

99.197

100

0.992
  mecA Streptococcus mutans UA159

57.787

100

0.588
  mecA Streptococcus pneumoniae TIGR4

48.594

100

0.494
  mecA Streptococcus pneumoniae Rx1

48.193

100

0.49
  mecA Streptococcus pneumoniae R6

48.193

100

0.49
  mecA Streptococcus pneumoniae D39

48.193

100

0.49

Multiple sequence alignment    



References

[1] Astrid Wahl et al. (2014) Control of natural transformation in salivarius Streptococci through specific degradation of σX by the MecA-ClpCP protease complex. Journal of Bacteriology 196(15):2807-16. [PMID: 24837292]
[2] Céline Boutry et al. (2012) Adaptor protein MecA is a negative regulator of the expression of late competence genes in Streptococcus thermophilus. Journal of Bacteriology 194(7):1777-88. [PMID: 22287513]