Detailed information    

experimental Experimentally validated

Overview

Name   pptB   Type   Regulator
Locus tag   STER_RS07725 Genome accession   NC_008532
Coordinates   1472993..1474030 (-) Length   345 a.a.
NCBI ID   WP_011681529.1    Uniprot ID   -
Organism   Streptococcus thermophilus LMD-9     
Function   export ComS   
Competence regulation

Function

PptAB controlled three regulation systems, two SHP/Rgg systems (SHP/Rgg1358 and SHP/Rgg1299), and the ComS/ComR system, while using transcriptional fusions and that PptAB helped to produce and export at least three different mature SHPs (SHP1358, SHP1299, and SHP279) peptides while using liquid chromatography-tandem mass spectrometry (LC-MS/MS).


Genomic Context

Location: 1467993..1479030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STER_RS07700 (STER_1566) ilvD 1468245..1469963 (-) 1719 WP_011226435.1 dihydroxy-acid dehydratase -
  STER_RS07705 (STER_1567) - 1470076..1471257 (+) 1182 WP_002951656.1 NAD(P)/FAD-dependent oxidoreductase -
  STER_RS07715 (STER_1569) trmB 1471493..1472134 (-) 642 WP_002947526.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  STER_RS07720 (STER_1570) ccrZ 1472144..1472935 (-) 792 WP_002951667.1 cell cycle regulator CcrZ -
  STER_RS07725 (STER_1571) - 1472993..1474027 (-) 1035 WP_024703983.1 ABC transporter permease -
  STER_RS07730 (STER_1572) pptA 1474027..1474758 (-) 732 WP_002951673.1 ABC transporter ATP-binding protein Regulator
  STER_RS07735 (STER_1573) - 1474823..1475242 (+) 420 WP_011681530.1 HIT family protein -
  STER_RS07740 (STER_1574) - 1475239..1475538 (+) 300 WP_011681531.1 hypothetical protein -
  STER_RS07745 (STER_1575) - 1475590..1476267 (-) 678 WP_011681532.1 hypothetical protein -
  STER_RS07750 (STER_1576) - 1476337..1476972 (-) 636 WP_011681533.1 DUF4230 domain-containing protein -
  STER_RS12065 (STER_1577) - 1476991..1477116 (-) 126 WP_011681534.1 hypothetical protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  pptB comS positive effect
  comS comX positive effect
  comX late competence genes positive effect
  comX late competence genes positive effect
  comR comX positive effect
  clpP comX negative effect
  mecA comX negative effect
  clpC comX negative effect
  comR comS positive effect
  comS comS positive effect
  amiD comS positive effect
  pptA comS positive effect
  amiE comS positive effect
  eeP comS positive effect
  amiA3 comS positive effect
  amiF comS positive effect
  amiC comS positive effect

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 40019.52 Da        Isoelectric Point: 10.5812

>NTDB_id=482 STER_RS07725 WP_011681529.1 1472993..1474030(-) (pptB) [Streptococcus thermophilus LMD-9]
MAELFKERSRRFTTRCAKYSRYVFNDHFILVLLFLLGFVLVQYSQLLRHFPKNPWAIILGLLVLCLLLPFWGNIATYLEP
ADKHYLLVKEEEVLDHIKKATGRAFRFWVLIQTLIFILVVPLFLALGLPVWGVVLIAVAMAILKYFIFQKKAQPFYKQSG
LNWTDAIAAENKRQQSILKFFSLFTNVKGITSSVKRRGYLDGILKRTKKDKKYTWYNLYLRAFLRSGDYVALSLRLFALT
LLVIFLVPEKWLAMVLVVVFDYLLLFQLTALKSHFAYQRMANLMPIGKDMQVSNLKRLINQIVLVMTLIQALCLFDLKFS
LILIGVMLVLSLVYLPAKLKKMID

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=482 STER_RS07725 WP_011681529.1 1472993..1474030(-) (pptB) [Streptococcus thermophilus LMD-9]
ATGATAGCTGAGCTTTTTAAGGAGCGTAGCAGACGATTCACTACCCGTTGTGCTAAGTATAGTCGCTACGTCTTTAATGA
CCACTTTATCCTGGTTCTTCTTTTTTTGCTGGGTTTTGTCTTGGTTCAGTATAGCCAGTTGCTCCGACATTTTCCAAAGA
ATCCATGGGCCATCATTCTTGGCTTACTTGTGCTTTGTCTTTTACTTCCTTTTTGGGGTAATATTGCCACCTATTTAGAG
CCTGCAGACAAGCACTACCTCTTAGTTAAGGAAGAAGAGGTGCTAGACCATATTAAGAAGGCGACAGGGCGTGCTTTTAG
ATTTTGGGTACTCATCCAGACCTTGATTTTTATCTTGGTCGTTCCACTCTTTCTGGCCTTGGGTCTTCCAGTTTGGGGAG
TTGTCCTTATAGCCGTGGCTATGGCTATTCTTAAGTATTTTATTTTCCAAAAGAAAGCACAACCTTTTTATAAGCAGTCT
GGATTGAATTGGACTGATGCTATTGCAGCGGAGAATAAACGACAACAATCAATCTTGAAGTTTTTCTCACTCTTTACCAA
TGTTAAGGGAATTACATCGAGTGTTAAACGCCGTGGCTATTTGGATGGCATTCTCAAGCGTACAAAGAAAGACAAGAAAT
ATACGTGGTACAACCTGTACCTAAGAGCCTTCTTACGTTCAGGAGATTACGTTGCCCTTAGCCTACGCCTTTTTGCTTTA
ACACTTTTGGTTATTTTTTTGGTTCCTGAAAAATGGCTGGCTATGGTACTTGTGGTTGTTTTTGACTATCTCTTACTCTT
CCAGCTCACAGCGCTTAAATCGCACTTTGCTTACCAAAGGATGGCCAACCTTATGCCAATTGGAAAAGACATGCAGGTGA
GCAATCTTAAGCGCTTGATTAATCAAATTGTCCTTGTAATGACACTGATTCAGGCTCTTTGTCTCTTTGACCTTAAATTT
AGCCTGATTTTGATTGGAGTTATGCTAGTTTTGAGTCTTGTTTACCTACCAGCCAAGCTTAAGAAGATGATTGACTAA

Domains

Predicted by InterproScan.

Bacterial ABC transporter protein EcsB (EcsB)

(3-159)

Bacterial ABC transporter protein EcsB (EcsB)

(157-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pptB Streptococcus salivarius strain HSISS4

92.733

100

0.927

Multiple sequence alignment    



References

[1] Abarna Lingeswaran et al. (2020) Export of Rgg Quorum Sensing Peptides is Mediated by the PptAB ABC Transporter in Streptococcus Thermophilus Strain LMD-9. Genes 11(9):1096. [PMID: 32961685]