Detailed information    

experimental Experimentally validated

Overview

Name   pptB   Type   Regulator
Locus tag   HSISS4_RS07765 Genome accession   NZ_CP013216
Coordinates   1715534..1716568 (-) Length   344 a.a.
NCBI ID   WP_418080400.1    Uniprot ID   -
Organism   Streptococcus salivarius strain HSISS4     
Function   export ComS   
Competence regulation

Function

We have shown the necessity for a pheromone exporter (PptAB) and a peptide importer (Ami/Opp) for competence activation through ComR overproduction.


Genomic Context

Location: 1710534..1721568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSISS4_RS07740 (HSISS4_01514) ilvD 1710767..1712485 (-) 1719 WP_021143714.1 dihydroxy-acid dehydratase -
  HSISS4_RS07745 (HSISS4_01515) - 1712598..1713779 (+) 1182 WP_021143713.1 NAD(P)/FAD-dependent oxidoreductase -
  HSISS4_RS07755 (HSISS4_01516) trmB 1714034..1714675 (-) 642 WP_021143712.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  HSISS4_RS07760 (HSISS4_01517) ccrZ 1714685..1715476 (-) 792 WP_002884270.1 cell cycle regulator CcrZ -
  HSISS4_RS07765 (HSISS4_01518) pptB 1715534..1716568 (-) 1035 WP_418080400.1 ABC transporter permease Regulator
  HSISS4_RS07770 (HSISS4_01519) pptA 1716568..1717299 (-) 732 WP_021143710.1 ABC transporter ATP-binding protein Regulator
  HSISS4_RS07775 (HSISS4_01520) - 1717364..1717783 (+) 420 WP_002891660.1 HIT family protein -
  HSISS4_RS07780 (HSISS4_01521) - 1717780..1718079 (+) 300 WP_021143709.1 hypothetical protein -
  HSISS4_RS07785 (HSISS4_01522) - 1718111..1718887 (-) 777 WP_021143708.1 FxLYD domain-containing protein -
  HSISS4_RS07790 (HSISS4_01523) - 1719321..1721426 (-) 2106 WP_021143707.1 ATP-dependent Clp protease ATP-binding subunit -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  pptB comS positive effect
  comS comX/sigX positive effect
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect
  stkP/pknB comX/sigX positive effect
  comR comX/sigX positive effect
  covS comR negative effect
  covR comR negative effect
  pptA comS positive effect
  amiD comS positive effect
  amiE comS positive effect
  amiC comS positive effect
  amiF comS positive effect
  pepF comS negative effect
  amiA comS positive effect

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39848.41 Da        Isoelectric Point: 10.6425

>NTDB_id=474 HSISS4_RS07765 WP_418080400.1 1715534..1716568(-) (pptB) [Streptococcus salivarius strain HSISS4]
MAKLFKERSKRFNARCAKYSRYVFNDHFILVLLFLLGFVLVQYSQLLKHFPKNPWAIILGLLVLCLLLPFWGNIATYLEP
ADKHYLLVKEEEVLDHVKKATGRAFRFWVIVQTLIFILVIPLFLALGLPVWGVILVAVAMAILKCFIFQKKAQPFYKQSG
LNWTKAIAGENKRQQSILKFFSLFTNVKGITSSVKRRAYLDGILKRTKKDKEHTWYNLYLRAFLRSGDYFALSLRLFALT
LLVIFLVPEKWLAMVLVVVFDYLLLFQLTALKSHFAYQRMANLMPVGKDMQVSNLKRLITQIVLVLTLVQAICLFDLKFS
LILVGIMLVLSLVYLPAKLKKMID

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=474 HSISS4_RS07765 WP_418080400.1 1715534..1716568(-) (pptB) [Streptococcus salivarius strain HSISS4]
ATAGCTAAGCTATTTAAGGAGCGTAGCAAGCGATTCAATGCCCGCTGTGCTAAGTATAGTCGCTACGTTTTTAATGATCA
TTTTATTTTGGTTCTTCTCTTTCTGCTAGGCTTTGTCTTGGTTCAGTATAGCCAGTTGCTGAAGCATTTTCCAAAAAATC
CGTGGGCCATCATTCTTGGCCTGCTTGTGCTTTGTCTGTTATTACCTTTCTGGGGGAATATTGCGACTTATCTAGAACCT
GCGGACAAGCACTACCTTTTGGTTAAGGAAGAAGAGGTGCTAGACCATGTTAAGAAGGCAACGGGGCGTGCTTTTAGATT
TTGGGTAATCGTACAAACCCTGATTTTTATTTTGGTAATTCCCCTCTTTTTAGCCTTGGGGCTGCCAGTTTGGGGAGTTA
TCCTTGTAGCTGTGGCAATGGCCATTCTTAAGTGCTTTATTTTCCAGAAAAAGGCTCAACCTTTTTATAAGCAGTCTGGA
CTGAATTGGACTAAGGCTATTGCAGGAGAAAATAAGCGTCAACAATCGATTCTGAAGTTCTTTTCACTCTTTACCAATGT
TAAGGGGATTACGTCAAGTGTTAAACGTCGTGCTTATTTGGATGGTATTCTCAAACGTACAAAGAAAGACAAGGAACATA
CCTGGTACAATCTTTACCTAAGAGCCTTCTTACGTTCTGGAGATTACTTTGCTCTTAGCCTACGTCTTTTTGCCCTAACG
CTTTTAGTTATCTTTTTGGTTCCTGAAAAATGGCTGGCTATGGTGCTTGTGGTTGTTTTTGACTACCTTTTGCTCTTCCA
GCTCACGGCGCTTAAATCGCACTTTGCTTATCAGAGAATGGCTAACCTTATGCCAGTTGGAAAAGACATGCAGGTGAGCA
ATCTTAAGCGCTTGATTACACAGATTGTACTTGTGTTGACTCTGGTACAGGCTATTTGTCTCTTCGACCTTAAATTTAGC
CTGATTTTGGTTGGAATTATGCTAGTTTTGAGCCTTGTTTACCTACCAGCCAAGCTTAAGAAGATGATTGACTAA

Domains

Predicted by InterproScan.

Bacterial ABC transporter protein EcsB (EcsB)

(3-159)

Bacterial ABC transporter protein EcsB (EcsB)

(157-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pptB Streptococcus thermophilus LMD-9

92.733

100

0.927

Multiple sequence alignment    



References

[1] Adrien Knoops et al. (2022) The CovRS Environmental Sensor Directly Controls the ComRS Signaling System To Orchestrate Competence Bimodality in Salivarius Streptococci. MBio 13(1):e0312521. [PMID: 35089064]