Detailed information    

experimental Experimentally validated

Overview

Name   amiD   Type   Regulator
Locus tag   HSISS4_RS06990 Genome accession   NZ_CP013216
Coordinates   1558665..1559591 (-) Length   308 a.a.
NCBI ID   WP_021143810.1    Uniprot ID   -
Organism   Streptococcus salivarius strain HSISS4     
Function   internalize XIP   
Competence regulation

Function

We have shown the necessity for a pheromone exporter (PptAB) and a peptide importer (Ami/Opp) for competence activation through ComR overproduction.


Genomic Context

Location: 1553665..1564591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSISS4_RS06960 (HSISS4_01357) - 1553743..1554537 (-) 795 WP_021143816.1 HAD-IIB family hydrolase -
  HSISS4_RS06965 (HSISS4_01358) - 1554719..1555096 (+) 378 WP_021143815.1 GntR family transcriptional regulator -
  HSISS4_RS06970 (HSISS4_01359) - 1555101..1555799 (+) 699 WP_021143814.1 ABC transporter ATP-binding protein -
  HSISS4_RS06975 (HSISS4_01360) - 1555811..1556596 (+) 786 WP_021143813.1 hypothetical protein -
  HSISS4_RS06980 (HSISS4_01361) amiF 1556648..1557577 (-) 930 WP_021143812.1 ATP-binding cassette domain-containing protein Regulator
  HSISS4_RS06985 (HSISS4_01362) amiE 1557570..1558655 (-) 1086 WP_021143811.1 ABC transporter ATP-binding protein Regulator
  HSISS4_RS06990 (HSISS4_01363) amiD 1558665..1559591 (-) 927 WP_021143810.1 oligopeptide ABC transporter permease OppC Regulator
  HSISS4_RS06995 (HSISS4_01364) amiC 1559591..1561084 (-) 1494 WP_013990225.1 ABC transporter permease Regulator
  HSISS4_RS07000 (HSISS4_01365) amiA 1561145..1563115 (-) 1971 WP_013990224.1 peptide ABC transporter substrate-binding protein Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  amiD comS positive effect
  comS comX/sigX positive effect
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect
  stkP/pknB comX/sigX positive effect
  comR comX/sigX positive effect
  covS comR negative effect
  covR comR negative effect
  pptA comS positive effect
  pptB comS positive effect
  amiE comS positive effect
  amiC comS positive effect
  amiF comS positive effect
  pepF comS negative effect
  amiA comS positive effect

Sequence

Protein


Download         Length: 308 a.a.        Molecular weight: 34595.72 Da        Isoelectric Point: 9.7054

>NTDB_id=477 HSISS4_RS06990 WP_021143810.1 1558665..1559591(-) (amiD) [Streptococcus salivarius strain HSISS4]
MASIDKSKFQFVKRDDFASETIDAPAYSYWKSVMRQFFKKKSTTVMLGILIAIILMSFIYPMFSKFDFNDVSKVNDFSVR
YVHPNAQYWFGTDSNGKSLFDSVWFGARNSILIAVIATFINVVIGLVVGAVWGISKTFDMIMMEIYNIISNIPALLVVIV
LTYSLGAGFWNMIFAMTVTGWIGIAYTIRIQIMRYRDLEYNLASRTLGTPTAKIVVKNIMPQLVSVIVTMASQLLPGFIS
YEAFLSYFGLGLPVTTPSLGRLISDYAQNVTVNAYLFWIPLTTLILVSLALFIVGQNLADASDPRTHR

Nucleotide


Download         Length: 927 bp        

>NTDB_id=477 HSISS4_RS06990 WP_021143810.1 1558665..1559591(-) (amiD) [Streptococcus salivarius strain HSISS4]
ATGGCTTCAATTGATAAAAGTAAGTTCCAATTTGTAAAGCGCGATGATTTTGCCTCTGAAACAATTGATGCGCCGGCCTA
TTCATACTGGAAATCGGTGATGCGTCAGTTCTTCAAGAAAAAGTCAACAACTGTCATGTTGGGAATCTTGATTGCTATCA
TTTTGATGAGCTTTATCTACCCAATGTTTTCAAAATTTGACTTTAATGACGTGTCTAAGGTTAATGACTTTAGTGTACGT
TATGTGCATCCAAATGCTCAATACTGGTTCGGTACGGACAGTAATGGTAAGTCTCTCTTTGACAGTGTTTGGTTTGGAGC
TCGTAATTCTATCCTTATTGCTGTTATCGCTACCTTCATTAACGTTGTTATTGGTTTAGTCGTTGGTGCTGTTTGGGGGA
TTTCTAAGACCTTTGATATGATTATGATGGAGATCTACAACATCATCTCAAACATTCCTGCCCTCCTTGTCGTTATCGTC
TTGACTTACTCATTGGGTGCAGGTTTCTGGAACATGATTTTTGCCATGACTGTCACAGGTTGGATTGGTATTGCTTATAC
TATCCGTATTCAAATCATGCGTTACCGTGATTTGGAGTACAACTTGGCTTCTCGTACTTTGGGAACACCAACTGCTAAGA
TTGTGGTTAAAAACATCATGCCGCAACTTGTGTCAGTTATCGTAACTATGGCTAGTCAACTCTTGCCAGGATTTATCTCT
TATGAAGCCTTCCTTTCATACTTTGGTTTGGGGCTTCCCGTTACGACGCCTAGTCTTGGTCGTTTGATTTCAGACTACGC
ACAGAACGTTACAGTCAATGCTTATCTCTTCTGGATTCCATTGACTACCTTGATTCTCGTTTCCCTTGCTCTCTTTATTG
TCGGTCAAAACTTGGCGGATGCCAGCGACCCACGTACGCATAGATAG

Domains

Predicted by InterproScan.

Binding-protein-dependent transport system inner membrane component (BPD_transp_1)

(125-306)

N-terminal TM domain of oligopeptide transport permease C (OppC_N)

(28-70)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiD Streptococcus thermophilus LMD-9

96.429

100

0.964
  amiD Streptococcus thermophilus LMG 18311

96.429

100

0.964

Multiple sequence alignment    



References

[1] Adrien Knoops et al. (2022) The CovRS Environmental Sensor Directly Controls the ComRS Signaling System To Orchestrate Competence Bimodality in Salivarius Streptococci. MBio 13(1):e0312521. [PMID: 35089064]