Detailed information NTG0000475

Experimentally validated

Overview

• Name: amiA
• Type: Regulator
• Locus tag: HSISS4_RS07005
• Genome accession: NZ_CP013216
• Coordinates: 1563297..1565267 (-)
• Length: 656 a.a.
• NCBI ID: WP_021143809.1
• Uniprot ID: -
• Organism: Streptococcus salivarius strain HSISS4
• Function: internalize XIP Competence regulation

Function

We have shown the necessity for a pheromone exporter (PptAB) and a peptide importer (Ami/Opp) for competence activation through ComR overproduction.

Genomic Context

Location: 1558297..1570267

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HSISS4_RS06990 (HSISS4_01363)	amiD	1558665..1559591 (-)	927	WP_021143810.1	oligopeptide ABC transporter permease OppC	Regulator
HSISS4_RS06995 (HSISS4_01364)	amiC	1559591..1561084 (-)	1494	WP_013990225.1	ABC transporter permease	Regulator
HSISS4_RS07000 (HSISS4_01365)	amiA	1561145..1563115 (-)	1971	WP_013990224.1	peptide ABC transporter substrate-binding protein	Regulator
HSISS4_RS07005 (HSISS4_01366)	amiA	1563297..1565267 (-)	1971	WP_021143809.1	peptide ABC transporter substrate-binding protein	Regulator
HSISS4_RS07010 (HSISS4_01367)	pta	1565503..1566486 (-)	984	WP_021143808.1	phosphate acetyltransferase	-
HSISS4_RS07015 (HSISS4_01368)	-	1566500..1567396 (-)	897	WP_021143807.1	RluA family pseudouridine synthase	-
HSISS4_RS07020 (HSISS4_01369)	-	1567393..1568229 (-)	837	WP_002884507.1	NAD kinase	-
HSISS4_RS07025 (HSISS4_01370)	-	1568201..1568875 (-)	675	WP_014634828.1	GTP pyrophosphokinase	-
HSISS4_RS07030 (HSISS4_01371)	-	1568971..1569546 (+)	576	WP_002886838.1	CYTH domain-containing protein	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
amiA	comS	positive effect
comS	comX/sigX	positive effect
comX/sigX	late competence genes	positive effect
comX/sigX	late competence genes	positive effect
stkP/pknB	comX/sigX	positive effect
comR	comX/sigX	positive effect
covS	comR	negative effect
covR	comR	negative effect
pptA	comS	positive effect
amiD	comS	positive effect
pptB	comS	positive effect
amiE	comS	positive effect
amiC	comS	positive effect
amiF	comS	positive effect
pepF	comS	negative effect

Sequence

Protein

• Length: 656 a.a.
• Molecular weight: 71897.65 Da
• Isoelectric Point: 4.8663

>NTDB_id=475 HSISS4_RS07005 WP_021143809.1 1563297..1565267(-) (amiA) [Streptococcus salivarius strain HSISS4]
MKKSKWLALAGVALLSVGALAACSSKSSTSGTTYGYVYNSDPESLDYITSNTGPTKTAVTNGVDGLMEADKYGNLVPSVA
EDWSVSQDGLTYTYKIRKGVKWYTSDGEEYADVTAKDFVTGLKHAADGKAGALYLVQDSIAGLSDYLSGANKDFSNVGVK
AIDDYTLQYTLKKPEPYWNSKTTYGLLSPVNEDFLKNKGKDFGKSTDPTSILYNGPFLLKSLTAKSSIELTKNENYWDKK
NVHFDAIKLSYYDGSDQEALERGFTDGAYSLARVYPTSSNYSSVEKKYKDNIYYTAPGASTSAIGVNIDRQSYKFSAKKT
DAEKTSTKKALLNKDFRQSINFAIDRTAYQSQMNGKDGAALALRNLFVPSDFVSAGDKTFGDLVTEKMSSYGDEWSGVNF
TDGQDGLYNAEKAKTEFAKAKETLQADGVQFPIHLDLPVDQSSKINVAQAQSLKQTIEQSLGSDNVVIDVNQLSSDDIQN
ATLNAANAAAEDWDISNGVIWGPDYQDPSTYLDIFKTTSSENTKTFMGYDDPNNAAAAQVGLKDYDALLDSAASETTDLN
ARYDRYAQAQAWLEDSSLVIPLTVGNGAAPVISRLTPFTGASMQVGDKNSSDYFKYVKPQEKVVTKKEYEQSREKWLKEK
KASNEKAQKDLEKHVK

Nucleotide

• Length: 1971 bp

>NTDB_id=475 HSISS4_RS07005 WP_021143809.1 1563297..1565267(-) (amiA) [Streptococcus salivarius strain HSISS4]
ATGAAAAAAAGCAAATGGTTAGCCCTCGCGGGTGTGGCACTCCTCAGTGTAGGTGCCTTAGCGGCTTGTTCTTCTAAGTC
AAGTACTAGTGGAACGACTTATGGTTATGTTTATAATTCAGACCCAGAATCATTGGATTACATCACTTCAAATACAGGAC
CTACTAAGACAGCTGTGACAAACGGTGTAGATGGTCTTATGGAAGCAGATAAATATGGAAATCTTGTCCCTTCAGTAGCT
GAAGATTGGTCTGTGTCTCAAGATGGTCTGACTTATACTTATAAAATCCGTAAGGGAGTTAAATGGTATACTTCTGATGG
TGAAGAGTATGCTGATGTGACTGCTAAGGACTTTGTGACTGGTCTTAAGCATGCGGCTGATGGCAAGGCTGGAGCTCTTT
ACTTGGTGCAAGATTCTATTGCTGGCTTGTCTGACTACCTCTCTGGTGCCAATAAAGATTTCTCAAATGTAGGCGTTAAA
GCAATCGACGATTATACATTGCAATACACCCTTAAAAAACCAGAGCCATACTGGAACTCTAAGACAACATATGGTTTGCT
CTCCCCAGTTAATGAAGACTTTCTCAAGAACAAAGGAAAAGATTTTGGTAAGTCAACAGACCCAACATCTATCCTCTATA
ATGGTCCTTTCCTTCTCAAATCTTTGACAGCTAAATCATCTATTGAGTTGACTAAGAATGAGAATTATTGGGACAAGAAG
AATGTACACTTTGATGCCATCAAATTGTCATATTATGACGGTTCGGATCAAGAGGCTCTAGAACGTGGTTTCACGGATGG
TGCCTATAGTTTGGCTCGTGTCTACCCAACGAGTTCTAACTATTCTTCTGTAGAGAAGAAATATAAAGATAACATTTATT
ACACAGCACCAGGTGCGTCTACATCAGCCATTGGTGTTAATATCGACCGCCAAAGCTATAAATTCTCAGCCAAGAAGACG
GATGCTGAAAAGACATCAACTAAGAAGGCTCTTCTAAACAAAGACTTCCGTCAATCAATCAACTTCGCAATTGATCGTAC
GGCTTATCAATCACAAATGAATGGTAAAGATGGTGCAGCTCTAGCTCTACGTAACCTCTTTGTTCCTTCTGATTTTGTCA
GTGCAGGTGATAAGACCTTTGGAGATCTTGTAACAGAGAAAATGTCCTCTTATGGTGATGAATGGTCAGGTGTAAACTTT
ACTGATGGTCAAGATGGCCTCTACAATGCTGAAAAAGCTAAGACTGAGTTTGCCAAAGCTAAGGAAACTCTCCAAGCAGA
TGGTGTTCAATTCCCAATTCACTTGGACCTTCCTGTTGACCAAAGTTCTAAAATCAATGTTGCTCAGGCCCAATCTTTGA
AACAAACCATTGAGCAATCATTGGGTAGCGATAATGTGGTTATCGATGTTAACCAATTATCATCTGATGACATACAAAAT
GCAACCCTTAACGCGGCTAATGCAGCGGCAGAAGACTGGGATATTTCAAACGGTGTCATTTGGGGTCCAGACTATCAAGA
CCCATCAACTTACCTTGATATCTTTAAGACAACTTCAAGTGAAAATACCAAGACTTTCATGGGGTATGATGATCCAAACA
ATGCTGCCGCAGCTCAAGTAGGTCTTAAAGATTATGATGCTTTGCTTGATTCAGCAGCTTCAGAAACAACAGACCTCAAC
GCTCGTTACGATCGTTATGCTCAAGCCCAAGCTTGGTTGGAAGATAGCTCTCTTGTCATTCCATTGACTGTTGGTAACGG
TGCAGCACCAGTTATCTCACGTTTGACACCATTTACGGGTGCTAGCATGCAGGTGGGTGATAAGAACTCATCAGACTACT
TCAAATACGTGAAACCACAAGAAAAAGTAGTTACTAAGAAAGAATATGAACAAAGTCGTGAAAAATGGCTTAAAGAAAAG
AAAGCATCTAACGAAAAAGCTCAAAAAGATCTTGAGAAACACGTTAAATAA

Domains

Predicted by InterproScan.

• Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5) (75-520)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiA3	Streptococcus thermophilus LMG 18311	90.563	100	0.907
amiA3	Streptococcus thermophilus LMD-9	90.411	100	0.905

References

• [1]: Adrien Knoops et al. (2022) The CovRS Environmental Sensor Directly Controls the ComRS Signaling System To Orchestrate Competence Bimodality in Salivarius Streptococci. MBio 13(1):e0312521. [PMID: 35089064]