Detailed information    

experimental Experimentally validated

Overview

Name   amiA3   Type   Regulator
Locus tag   STU_RS16140 Genome accession   NC_006448
Coordinates   1279859..1281832 (-) Length   657 a.a.
NCBI ID   WP_011226306.1    Uniprot ID   Q5M3H2
Organism   Streptococcus thermophilus LMG 18311     
Function   binding to XIP   
Competence regulation

Function

XIP peptides are recognized by the oligopeptide-binding protein AmiA3 and internalized by the oligopeptide Ami transporter (AmiCDEF).


Genomic Context

Location: 1274859..1286832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STU_RS16130 (stu1442) amiA 1276242..1278209 (-) 1968 WP_011225333.1 peptide ABC transporter substrate-binding protein Regulator
  STU_RS16135 (stu1444) - 1278565..1279814 (+) 1250 Protein_1243 ISL3 family transposase -
  STU_RS16140 (stu1445) amiA3 1279859..1281832 (-) 1974 WP_011226306.1 peptide ABC transporter substrate-binding protein Regulator
  STU_RS19635 - 1282036..1282226 (-) 191 Protein_1245 IS3 family transposase -
  STU_RS16145 (stu1447) - 1282220..1282630 (-) 411 Protein_1246 ATP-binding cassette domain-containing protein -
  STU_RS20240 - 1282662..1282976 (-) 315 Protein_1247 TatD family hydrolase -
  STU_RS16155 (stu1450) - 1283326..1284531 (+) 1206 WP_011226310.1 L-lactate MFS transporter -
  STU_RS19640 - 1284577..1285928 (-) 1352 Protein_1249 IS3 family transposase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  amiA3 comS positive effect
  comS comS positive effect
  comS comX positive effect
  amiC comS positive effect
  eeP comS positive effect
  amiD comS positive effect
  comR comS positive effect
  amiF comS positive effect
  amiE comS positive effect
  comX ssbA positive effect
  comX comGE positive effect
  comX comGB positive effect
  comX comGD positive effect
  comX comGF positive effect
  comX comGG positive effect
  comX radA positive effect
  comX comEC positive effect
  comX comFC positive effect
  comX positive effect
  comX comEA positive effect
  comX comGC positive effect
  comX cinA positive effect
  comX coiA positive effect
  comX comGA positive effect
  comX comFA positive effect
  comX dprA positive effect
  clpC comX negative effect
  comR comX positive effect
  clpP comX negative effect
  mecA comX negative effect

Sequence

Protein


Download         Length: 657 a.a.        Molecular weight: 72286.26 Da        Isoelectric Point: 5.1005

>NTDB_id=324 STU_RS16140 WP_011226306.1 1279859..1281832(-) (amiA3) [Streptococcus thermophilus LMG 18311]
MKKSKWLALAGVALLGVGVLAACSSKSNTSGTTYGYVYSSDPETLDYITSNTGPTKSVVTNGVDGLMEADKYGNLVPSVA
EDWSVSKDGLTYTYKIRKGIKWYTSEGEEYANVTAKDFVTGLKHAADSKAGGIYLVQDSIAGLSDYLSGTNKDFSNVGVK
AIDEYTLQYTLKQPEPYWNSKTTYSLLFPVNEDFLKNKGKDFGKSTDPTSILYNGPFLLKSLTAKSSIELVKNEHYWDKK
NVHFDAIKFSYYDGSDQDALVRGFTDGAYNFARVFPTSSNYASVEKKYKDNIYYTAPGASTSAIGINLDRQSYKYTAKKT
DSEKSSTKKALLNKDFRQAINFAIDRKAYQSQINGKDGATLAVRNLFVPSDFVSAGDKTFGDLVTEKMSSYGDEWSGVNF
ADSQDGLYNAEKAKTEFAKAKDALQADGVQFPIHLDLPVDQSSKLNVAQAQSLKQTIEKSLGSENVVIDINQLSSDDLEN
VTLNAANAAAEDWDISNSVAWAPDYQDPSTYLDIFKTTSSENTKAFMGYDDPNNAAAAQVGLKDYDALLNSAASETSDLN
VRYDRYAQAQAWLEDSSLVIPLTVGNGAAPAISRLTPFTGASLQVGDKNSSNDYIKYVKPQEKVVTKKEYEQSREKWLKE
KKESNEKAQKDLEKHVK

Nucleotide


Download         Length: 1974 bp        

>NTDB_id=324 STU_RS16140 WP_011226306.1 1279859..1281832(-) (amiA3) [Streptococcus thermophilus LMG 18311]
ATGAAAAAAAGCAAATGGTTGGCTCTCGCGGGTGTAGCACTTCTAGGTGTTGGTGTGTTAGCGGCTTGTTCATCTAAATC
AAACACTAGCGGAACTACTTATGGTTATGTTTACAGTTCGGATCCAGAAACTTTGGATTATATTACATCAAATACGGGAC
CTACAAAATCTGTTGTGACAAATGGTGTAGATGGACTTATGGAAGCGGACAAATATGGTAATCTTGTTCCATCAGTAGCT
GAAGACTGGTCTGTGTCTAAAGATGGTCTTACTTATACCTATAAAATTCGTAAAGGGATCAAATGGTATACTTCTGAAGG
TGAAGAGTACGCTAATGTTACTGCTAAGGACTTTGTAACGGGTCTTAAACATGCTGCGGATTCCAAAGCAGGTGGTATCT
ATTTGGTTCAAGATTCTATTGCTGGATTGTCTGACTATCTCTCTGGTACCAATAAAGATTTCTCGAATGTGGGTGTCAAG
GCAATTGATGAGTATACTTTGCAGTACACGCTTAAGCAACCAGAACCATACTGGAACTCTAAGACAACTTATAGTTTGCT
TTTCCCAGTAAATGAAGACTTCCTCAAGAATAAGGGAAAAGATTTTGGTAAATCAACAGACCCAACCTCTATCCTTTATA
ATGGACCTTTCCTTCTCAAATCTTTGACAGCTAAATCTTCAATTGAGTTGGTTAAGAATGAACATTATTGGGACAAGAAA
AATGTACACTTTGATGCCATTAAATTCTCATATTATGATGGTTCGGATCAAGATGCTCTAGTACGTGGTTTCACTGATGG
TGCTTATAATTTTGCTCGTGTCTTTCCAACGAGTTCAAACTATGCTTCTGTAGAGAAGAAATATAAAGATAATATTTATT
ACACAGCACCAGGTGCGTCTACATCAGCTATTGGTATTAATCTTGACCGTCAAAGTTACAAGTATACAGCCAAAAAGACG
GATTCTGAAAAATCATCAACCAAGAAAGCCCTTCTAAACAAGGACTTCCGTCAAGCAATTAACTTTGCCATTGACCGTAA
GGCCTATCAATCACAAATTAACGGTAAAGATGGTGCAACTCTAGCTGTTCGTAACCTCTTTGTGCCTTCTGATTTTGTGA
GTGCAGGTGATAAGACCTTCGGAGATCTTGTAACAGAGAAAATGTCTTCATATGGGGATGAATGGTCAGGTGTGAATTTT
GCTGATAGTCAAGATGGCCTCTACAATGCTGAAAAAGCTAAGACTGAGTTTGCCAAAGCTAAGGATGCTCTCCAAGCAGA
TGGTGTTCAATTCCCAATTCATTTGGACCTTCCAGTTGACCAAAGCTCTAAACTTAATGTTGCCCAAGCCCAATCTTTGA
AACAAACCATTGAAAAATCATTGGGTAGCGAAAATGTTGTTATAGATATTAACCAATTGTCATCTGATGATTTGGAAAAT
GTAACACTTAATGCAGCCAACGCAGCAGCAGAAGACTGGGATATTTCAAATAGTGTCGCTTGGGCGCCAGATTACCAAGA
TCCATCAACCTACCTTGATATCTTTAAGACAACTTCAAGTGAAAACACTAAGGCTTTCATGGGTTATGATGATCCAAATA
ATGCGGCAGCAGCCCAAGTGGGACTCAAAGACTATGATGCCTTGCTGAATTCTGCAGCCTCAGAAACAAGTGACCTTAAT
GTTCGTTACGATCGTTATGCTCAAGCTCAAGCATGGTTGGAGGACAGTTCGCTTGTGATTCCATTGACTGTTGGTAATGG
TGCGGCACCAGCTATCTCACGATTGACACCGTTTACCGGTGCTAGCCTCCAGGTGGGTGATAAAAACTCATCAAACGACT
ACATCAAATATGTGAAACCACAAGAAAAAGTAGTTACTAAGAAAGAATATGAACAAAGTCGTGAAAAATGGCTTAAAGAA
AAGAAAGAATCTAACGAAAAAGCTCAAAAAGATCTTGAGAAACACGTTAAATAA

Domains

Predicted by InterProScan.

Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5)

(75-520)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiA3 Streptococcus thermophilus LMD-9

98.478

100

0.985
  amiA Streptococcus salivarius strain HSISS4

90.563

100

0.907

Multiple sequence alignment    



References

[1] Rozenn Gardan et al. (2013) Extracellular life cycle of ComS, the competence-stimulating peptide of Streptococcus thermophilus. Journal of Bacteriology 195(8):1845-55. [PMID: 23396911]
[2] Laetitia Fontaine et al. (2010) A novel pheromone quorum-sensing system controls the development of natural competence in Streptococcus thermophilus and Streptococcus salivarius. Journal of Bacteriology 192(5):1444-54. [PMID: 20023010]
[3] Rozenn Gardan et al. (2009) The oligopeptide transport system is essential for the development of natural competence in Streptococcus thermophilus strain LMD-9. Journal of Bacteriology 191(14):4647-55. [PMID: 19447907]