Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   STU_RS17950 Genome accession   NC_006448
Coordinates   1629750..1631111 (-) Length   453 a.a.
NCBI ID   WP_082302317.1    Uniprot ID   -
Organism   Streptococcus thermophilus LMG 18311     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1624750..1636111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STU_RS20285 - 1625344..1626371 (-) 1028 Protein_1592 peptide ABC transporter ATP-binding protein -
  STU_RS17920 (stu1817) rplA 1626490..1627179 (-) 690 WP_002946412.1 50S ribosomal protein L1 -
  STU_RS17925 (stu1818) rplK 1627279..1627704 (-) 426 WP_002953535.1 50S ribosomal protein L11 -
  STU_RS17930 (stu1819) - 1627876..1628229 (+) 354 WP_002953536.1 DUF3397 domain-containing protein -
  STU_RS17935 (stu1820) - 1628321..1628818 (-) 498 WP_002947059.1 beta-class carbonic anhydrase -
  STU_RS19750 - 1628986..1629661 (-) 676 Protein_1597 AIM24 family protein -
  STU_RS17950 (stu1823) radA 1629750..1631111 (-) 1362 WP_082302317.1 DNA repair protein RadA Machinery gene
  STU_RS21165 - 1631147..1631288 (-) 142 Protein_1599 cysteine hydrolase -
  STU_RS17955 (stu1824) - 1631289..1631735 (-) 447 WP_002947069.1 dUTP diphosphatase -
  STU_RS17960 - 1631949..1632544 (+) 596 Protein_1601 NADPH-dependent FMN reductase -
  STU_RS17965 (stu1828) - 1632572..1633542 (+) 971 Protein_1602 PAS domain-containing protein -
  STU_RS19755 - 1633660..1634450 (+) 791 Protein_1603 formate/nitrite transporter family protein -
  STU_RS17980 (stu1831) - 1634459..1635032 (+) 574 Protein_1604 MptD family putative ECF transporter S component -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX radA positive effect
  comX ssbA positive effect
  comX comGE positive effect
  comX comGB positive effect
  comX comGD positive effect
  comX comGF positive effect
  comX comGG positive effect
  comX comEC positive effect
  comX comFC positive effect
  comX recA positive effect
  comX comEA positive effect
  comX comGC positive effect
  comX cinA positive effect
  comX coiA positive effect
  comX comGA positive effect
  comX comFA positive effect
  comX dprA positive effect
  clpC comX negative effect
  comR comX positive effect
  comR comS positive effect
  comS comX positive effect
  comS comS positive effect
  amiC comS positive effect
  eeP comS positive effect
  amiD comS positive effect
  amiF comS positive effect
  amiA3 comS positive effect
  amiE comS positive effect
  clpP comX negative effect
  mecA comX negative effect

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49450.86 Da        Isoelectric Point: 6.6528

>NTDB_id=349 STU_RS17950 WP_082302317.1 1629750..1631111(-) (radA) [Streptococcus thermophilus LMG 18311]
MAKKKSTFVCRECGYNSPKYLGRCPNCSSWSSFEEEVEMQEVKNARVSLTGEKSKPTKLKEVSSISYARIKTDMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAVRSQVE
GIKPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELIQIAKTNNIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGSRPILAEVQALVTPTVFGNAK
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKELPTNPQEAFVGEIGLTGEIR
RVTRIEQRINEAAKLGFTKIYVPKNSLHGMKIPEGIQVIGVTTVGEVLKKVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=349 STU_RS17950 WP_082302317.1 1629750..1631111(-) (radA) [Streptococcus thermophilus LMG 18311]
ATAGCTAAGAAAAAATCAACTTTTGTTTGTAGAGAATGTGGCTACAATTCACCCAAATATCTAGGGCGCTGTCCCAATTG
TTCTTCTTGGTCCTCTTTTGAGGAAGAGGTTGAGATGCAGGAGGTTAAAAATGCCCGTGTCAGTTTGACGGGTGAAAAGT
CCAAACCTACTAAGTTAAAAGAAGTCTCTTCAATTAGTTATGCCCGCATAAAAACCGATATGGACGAGTTTAACCGTGTT
TTAGGTGGCGGTGTGGTTCCGGGGAGTTTGGTTCTTATTGGAGGAGATCCAGGAATAGGGAAATCGACCCTCCTCTTACA
GGTGTCAACACAGCTGGCTAACAAGGGAACTGTCCTCTATGTTTCTGGGGAAGAATCGGCCGAGCAAATCAAATTGCGGA
GTGAACGTTTAGGTGATATTGATAACGAATTTTATCTGTATGCTGAGACCAATATGCAAGCAGTCCGTAGTCAAGTGGAG
GGTATCAAACCCGATTTCCTCATTATTGATTCTATTCAGACCATCATGAGCCCAGAGATCTCTGGCGTTCAAGGTTCTGT
TTCCCAGGTTCGTGAAGTAACTGCCGAACTCATACAGATTGCCAAAACCAATAACATTGCCACTTTCATTGTTGGGCATG
TGACCAAGGAGGGACAGCTGGCTGGACCACGTATGTTGGAGCACATGGTGGATACTGTGCTTTATTTTGAGGGGGAACGT
CATCATACCTTCCGTATTCTGAGGGCAGTTAAAAACCGTTTTGGTTCAACCAATGAGATTGGGATTTTTGAAATGCAGTC
AGGTGGTCTGGTCGAGGTACTAAACCCTAGCCAAGTTTTCTTAGAAGAACGCCTAGACGGTGCCACAGGTTCTGCCATTG
TTGTCACAATGGAGGGGAGTCGACCTATCCTAGCTGAGGTTCAAGCTTTGGTGACACCGACCGTCTTTGGTAACGCAAAG
CGGACAACGACGGGACTGGACTTTAACCGTGTCAGCCTTATCATGGCTGTCTTAGAGAAACGTTGCGGCCTTCTTCTCCA
AAATCAAGATGCCTATCTCAAATCAGCTGGTGGTGTCAAGTTGGACGAGCCAGCTATTGACCTTGCAGTTGCCGTTGCTA
TCGCTTCTAGTTACAAGGAGCTTCCAACCAATCCACAAGAAGCCTTCGTAGGAGAAATTGGCTTGACCGGAGAGATTCGT
CGTGTGACTCGTATTGAGCAGCGTATCAATGAAGCCGCTAAACTAGGTTTTACTAAAATTTATGTTCCTAAAAATTCGCT
TCATGGCATGAAGATTCCAGAAGGTATCCAGGTCATTGGGGTCACAACGGTAGGAGAAGTACTCAAAAAAGTCTTTTCTT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

88.742

100

0.887
  radA Streptococcus pneumoniae D39

88.742

100

0.887
  radA Streptococcus pneumoniae R6

88.742

100

0.887
  radA Streptococcus pneumoniae TIGR4

88.742

100

0.887
  radA Streptococcus mitis NCTC 12261

88.742

100

0.887
  radA Streptococcus mitis SK321

88.742

100

0.887
  radA Bacillus subtilis subsp. subtilis str. 168

61.148

100

0.611

Multiple sequence alignment