Detailed information NTG0000125

Experimentally validated

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: BSU_00870
• Genome accession: NC_000964
• Coordinates: 106096..107472 (+)
• Length: 458 a.a.
• NCBI ID: NP_387968.1
• Uniprot ID: P37572
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: homologous recombination Homologous recombination

Function

RadA is a conserved DNA repair protein with ATP-dependent DNA helicase activity. In natural transformation, RadA facilitates homologous recombination by promoting strand pairing and stabilizing recombination intermediates. It works alongside RecA to enhance the efficiency of exogenous DNA integration into the genome, particularly under conditions of replication stress or DNA damage.

Genomic Context

Location: 101096..112472

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSU_00830 (BSU00830)	ctsR	101449..101913 (+)	465	NP_387964.1	transcriptional regulator of class III stress genes	-
BSU_00840 (BSU00840)	mcsA	101927..102484 (+)	558	NP_387965.1	activator of protein kinase McsB	-
BSU_00850 (BSU00850)	mcsB	102484..103575 (+)	1092	NP_387966.1	protein arginine kinase	-
BSU_00860 (BSU00860)	clpC	103572..106004 (+)	2433	NP_387967.1	class III stress response-related ATPase, AAA+ superfamily	Regulator
BSU_00870 (BSU00870)	radA	106096..107472 (+)	1377	NP_387968.1	DNA repair protein; 6-O-methylguanine-DNA methyltransferase	Machinery gene
BSU_00880 (BSU00880)	disA	107476..108558 (+)	1083	NP_387969.1	diadenylate cyclase; DNA integrity scanning protein; cell cycle checkpoint DNA scanning protein	-
BSU_00890 (BSU00890)	yacL	108674..109774 (+)	1101	NP_387970.1	putative membrane protein possibly involved in RNA binding	-
BSU_00900 (BSU00900)	ispD	109789..110487 (+)	699	NP_387971.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, nonmevalonate isoprenoid pathway	-
BSU_00910 (BSU00910)	ispF	110480..110956 (+)	477	NP_387972.1	2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase	-

Sequence

Protein

• Length: 458 a.a.
• Molecular weight: 49482.99 Da
• Isoelectric Point: 8.2399

>NTDB_id=125 BSU_00870 NP_387968.1 106096..107472(+) (radA) [Bacillus subtilis subsp. subtilis str. 168]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPSFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIVISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide

• Length: 1377 bp

>NTDB_id=125 BSU_00870 NP_387968.1 106096..107472(+) (radA) [Bacillus subtilis subsp. subtilis str. 168]
ATGGCTAAAACAAAATCGAAGTTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
TGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCATCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCTGC
TCCAGGCAGTGTGTCACAGGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCACTCATCTCGCCAACAAGTTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
ACTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCGA
TTGTGATTAGTATTGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

• Subunit ChlI of Mg-chelatase (ChlI) (365-430)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• AAA domain (AAA_25) (72-222)

3D structure

• AlphaFold DB: P37572

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Streptococcus pneumoniae TIGR4	63.135	100	0.631
radA	Streptococcus pneumoniae R6	63.135	100	0.631
radA	Streptococcus pneumoniae D39	63.135	100	0.631
radA	Streptococcus pneumoniae Rx1	63.135	100	0.631
radA	Streptococcus mitis NCTC 12261	63.135	100	0.631
radA	Streptococcus mitis SK321	62.914	100	0.629

References

• [1]: Rubén Torres et al. (2023) Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/Sms. International Journal of Molecular Sciences 24(5):4536. [PMID: 36901969]
• [2]: Ester Serrano et al. (2021) Recombination proteins differently control the acquisition of homeologous DNA during Bacillus subtilis natural chromosomal transformation. Environmental Microbiology 23(1):512-524. [PMID: 33264457]
• [3]: Rubén Torres et al. (2021) Bacillus subtilis RecA, DisA, and RadA/Sms Interplay Prevents Replication Stress by Regulating Fork Remodeling. Frontiers in Microbiology 12:766897. [PMID: 34880841]
• [4]: Rubén Torres et al. (2019) Bacillus subtilis RadA/Sms contributes to chromosomal transformation and DNA repair in concert with RecA and circumvents replicative stress in concert with DisA. DNA Repair 77:45-57. [PMID: 30877841]
• [5]: Rubén Torres et al. (2019) Bacillus subtilis RecA interacts with and loads RadA/Sms to unwind recombination intermediates during natural chromosomal transformation. Nucleic Acids Research 47(17):9198-9215. [PMID: 31350886]
• [6]: B Carrasco et al. (2002) Effect of the recU suppressors sms and subA on DNA repair and homologous recombination in Bacillus subtilis. Molecular Genetics And Genomics : MGG 266(5):899-906. [PMID: 11810266]
• [7]: EIke Krüger et al. (1997) The Bacillus subtilis clpC operon encodes DNA repair and competence proteins. Microbiology (Reading, England) 143 ( Pt 4):1309-1316. [PMID: 9141693]