Detailed information    

experimental Experimentally validated

Overview

Name   comR   Type   Regulator
Locus tag   STU_RS10980 Genome accession   NC_006448
Coordinates   270090..270989 (+) Length   299 a.a.
NCBI ID   WP_011225448.1    Uniprot ID   Q5M612
Organism   Streptococcus thermophilus LMG 18311     
Function   activate transcription of comX; activate transcription of comS   
Competence regulation

Function

Once reimported, XIP then interacts with the transcriptional activator ComR and stimulates the binding of the ComR-XIP complex as a dimer to the ComR-box located in the promoter of comX and comS, thereby promoting their transcription


Genomic Context

Location: 265090..275989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STU_RS10960 (stu0266) - 266441..267394 (-) 954 WP_041826943.1 IS30 family transposase -
  STU_RS10965 (stu0267) - 267823..268362 (+) 540 WP_372585740.1 tRNA (cytidine(34)-2'-O)-methyltransferase -
  STU_RS10970 (stu0268) - 268674..269231 (+) 558 WP_002949523.1 ECF transporter S component -
  STU_RS10975 (stu0269) - 269234..269884 (+) 651 WP_011225447.1 phosphatase PAP2 family protein -
  STU_RS10980 (stu0270) comR 270090..270989 (+) 900 WP_011225448.1 helix-turn-helix domain-containing protein Regulator
  - comS 271077..271148 (+) 72 - - Regulator
  STU_RS10990 (stu0272) - 271227..272405 (+) 1179 Protein_238 ABC transporter transmembrane domain-containing protein -
  STU_RS11005 (stu0275) - 272691..273259 (+) 569 Protein_239 ATP-binding cassette domain-containing protein -
  STU_RS11010 (stu0276) - 273367..273684 (+) 318 WP_011225454.1 DUF805 domain-containing protein -
  STU_RS20385 (stu0277) - 274171..274791 (-) 621 WP_232087240.1 DUF4153 domain-containing protein -
  STU_RS11020 (stu0280) - 275472..275987 (+) 516 WP_002949547.1 AmiS/UreI family transporter -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comR comS positive effect
  comS comS positive effect
  comS comX positive effect
  amiC comS positive effect
  eeP comS positive effect
  amiD comS positive effect
  amiF comS positive effect
  amiA3 comS positive effect
  amiE comS positive effect
  comX ssbA positive effect
  comX comGE positive effect
  comX comGB positive effect
  comX comGD positive effect
  comX comGF positive effect
  comX comGG positive effect
  comX radA positive effect
  comX comEC positive effect
  comX comFC positive effect
  comX recA positive effect
  comX comEA positive effect
  comX comGC positive effect
  comX cinA positive effect
  comX coiA positive effect
  comX comGA positive effect
  comX comFA positive effect
  comX dprA positive effect
  clpC comX negative effect
  comR comX positive effect
  clpP comX negative effect
  mecA comX negative effect

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 35303.77 Da        Isoelectric Point: 5.1073

>NTDB_id=321 STU_RS10980 WP_011225448.1 270090..270989(+) (comR) [Streptococcus thermophilus LMG 18311]
MNIKDSIGLRIKTERERQQMSREVLCLDGAELTVRQLIRIEKGESLPSLDRLSYIAKRLGKSMTELLDQDNITIPDEYYE
MKNRLIKFPTYRNPDRIKSKLTLIEEVYEKFFDILPEEELLTLDILENILSFTSWEESPKVEEIYEDLFEQVKRKRKFST
NDLLVIDYYFFHLYGRKQYDKKLFERIIKRVLNQEIWTDDVYNIVLFNDLMAIAALKIFHNSFSDFLTVVDKALAVIEKS
QLYSYKPSVFVLKAKYELLHKENKKEAAENYDKAIMFASVLEDSVLEESIKAGKLADGL

Nucleotide


Download         Length: 900 bp        

>NTDB_id=321 STU_RS10980 WP_011225448.1 270090..270989(+) (comR) [Streptococcus thermophilus LMG 18311]
TTGAACATAAAAGACAGCATTGGACTAAGAATCAAAACTGAGCGTGAACGCCAACAGATGTCACGTGAAGTGCTATGTTT
AGATGGTGCGGAATTGACTGTTCGCCAGTTAATTCGTATTGAAAAGGGGGAGTCTCTCCCGTCTTTAGATAGATTATCGT
ATATTGCTAAACGTTTAGGAAAAAGTATGACAGAGTTATTGGATCAAGACAATATTACCATTCCTGACGAATATTATGAA
ATGAAGAATCGTTTGATTAAGTTTCCAACGTACAGAAACCCTGACAGAATAAAGTCTAAACTTACTTTGATTGAGGAAGT
CTATGAGAAATTTTTTGATATTCTTCCAGAAGAAGAATTATTAACTCTAGACATTCTCGAAAATATATTGAGTTTTACTA
GCTGGGAGGAGAGTCCAAAAGTTGAGGAGATATATGAAGACTTGTTTGAACAAGTCAAAAGGAAGAGGAAATTCTCAACT
AACGATTTATTAGTCATTGACTATTATTTCTTTCATCTTTATGGGAGAAAACAGTATGACAAAAAACTATTTGAAAGAAT
TATAAAGAGAGTATTAAATCAGGAAATTTGGACAGATGATGTTTACAATATTGTTTTATTTAATGATTTGATGGCTATTG
CTGCTTTAAAGATTTTTCACAATTCCTTCTCAGACTTCTTAACAGTTGTGGATAAAGCCTTAGCTGTCATAGAAAAATCA
CAATTATATAGCTACAAGCCTAGTGTTTTTGTACTTAAGGCTAAATATGAACTTCTGCATAAAGAAAACAAGAAAGAGGC
TGCAGAGAATTATGATAAGGCCATAATGTTTGCTTCCGTTTTGGAAGACTCGGTTTTAGAGGAAAGTATAAAGGCAGGAA
AATTGGCAGATGGTTTATAG

Domains

Predicted by InterproScan.

ComR tetratricopeptide (ComR_TPR)

(75-292)

Helix-turn-helix (HTH_3)

(11-66)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M612

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comR Streptococcus thermophilus LMD-9

99.331

100

0.993
  comR Streptococcus salivarius strain HSISS4

95.318

100

0.953
  comR Streptococcus salivarius SK126

94.649

100

0.946
  comR/comR2 Streptococcus sobrinus strain NIDR 6715-7

42.14

100

0.421
  comR/comR1 Streptococcus sobrinus strain NIDR 6715-7

42.14

100

0.421

Multiple sequence alignment    



References

[1] Laura Ledesma-García et al. (2021) Coevolution of the bacterial pheromone ComS and sensor ComR fine-tunes natural transformation in streptococci. The Journal of Biological Chemistry 297(6):101346. [PMID: 34715127]
[2] Laura Ledesma-Garcia et al. (2020) Molecular dissection of pheromone selectivity in the competence signaling system ComRS of streptococci. Proceedings of The National Academy of Sciences of The United States of America 117(14):7745-7754. [PMID: 32198205]
[3] Antoine Talagas et al. (2016) Structural Insights into Streptococcal Competence Regulation by the Cell-to-Cell Communication System ComRS. PLoS Pathogens 12(12):e1005980. [PMID: 27907189]
[4] Laurie Haustenne et al. (2015) Modeling of the ComRS Signaling Pathway Reveals the Limiting Factors Controlling Competence in Streptococcus thermophilus. Frontiers in Microbiology 6:1413. [PMID: 26733960]
[5] Laetitia Fontaine et al. (2013) Mechanism of competence activation by the ComRS signalling system in streptococci. Molecular Microbiology 87(6):1113-32. [PMID: 23323845]
[6] Rozenn Gardan et al. (2013) Extracellular life cycle of ComS, the competence-stimulating peptide of Streptococcus thermophilus. Journal of Bacteriology 195(8):1845-55. [PMID: 23396911]
[7] Laetitia Fontaine et al. (2010) A novel pheromone quorum-sensing system controls the development of natural competence in Streptococcus thermophilus and Streptococcus salivarius. Journal of Bacteriology 192(5):1444-54. [PMID: 20023010]