Detailed information NTG0000580

Experimentally validated

Overview

• Name: comR/comR2
• Type: Regulator
• Locus tag: DLJ51_RS00275
• Genome accession: NZ_CP029560
• Coordinates: 42279..43181 (+)
• Length: 300 a.a.
• NCBI ID: WP_109982339.1
• Uniprot ID: -
• Organism: Streptococcus sobrinus strain NIDR 6715-7
• Function: require for natural transformation Unclear

Function

Transformation required at least 1 copy of comR2, and the transformation efficiency was inversely correlated with the number of copies of comR1.

Genomic Context

Location: 37279..48181

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DLJ51_RS00260 (DLJ51_00260)	-	38479..38637 (+)	159	WP_019768924.1	bacteriocin	-
DLJ51_RS00265 (DLJ51_00265)	comR/comR1	38804..39709 (+)	906	WP_109982338.1	XRE family transcriptional regulator	Regulator
-	comS	39857..39922 (+)	66	-	-	Regulator
DLJ51_RS00270 (DLJ51_00270)	comA	39969..42086 (+)	2118	WP_019772678.1	peptide cleavage/export ABC transporter	Regulator
DLJ51_RS00275 (DLJ51_00275)	comR/comR2	42279..43181 (+)	903	WP_109982339.1	XRE family transcriptional regulator	Regulator
DLJ51_RS00280 (DLJ51_00280)	-	43311..44183 (-)	873	WP_109982340.1	helix-turn-helix domain-containing protein	-
DLJ51_RS00285 (DLJ51_00285)	-	44502..45260 (+)	759	WP_019768919.1	ABC transporter ATP-binding protein	-
DLJ51_RS00290 (DLJ51_00290)	-	45263..47572 (+)	2310	WP_109982341.1	ABC transporter permease	-

Sequence

Protein

• Length: 300 a.a.
• Molecular weight: 35453.99 Da
• Isoelectric Point: 5.3662

>NTDB_id=580 DLJ51_RS00275 WP_109982339.1 42279..43181(+) (comR/comR2) [Streptococcus sobrinus strain NIDR 6715-7]
MTMKEVLGQRIKEERESKQISQKLLCGNGAALTVRQLQRIEHGQSLPTLEKLEFIARRLDLKTSDLLESVDVQLPEDYWA
MKARIVKFPTYGEKERIQRKLDLINEVLVKYFDRLPEDELLFLDLSENILDRIHDKRIPNIEEIYDDAFEQVLKKKTFTF
NDYLYLSYFLQKCRNEASYDRKIFKQIEQKLLQQELSSDEFYNIEFLAAVMDAAAVYAFHDDYKDIMPLLKKIHWIIEQA
QLHTYKAGVLELEAKYYVKIAKDKDRAKKLYQQALKLGEILEDPAIIADIKMEMKNDGIE

Nucleotide

• Length: 903 bp

>NTDB_id=580 DLJ51_RS00275 WP_109982339.1 42279..43181(+) (comR/comR2) [Streptococcus sobrinus strain NIDR 6715-7]
ATGACGATGAAAGAAGTTCTAGGGCAGCGGATTAAGGAAGAAAGAGAAAGCAAGCAGATCTCACAGAAGCTCCTATGCGG
GAATGGGGCAGCTCTGACTGTCCGCCAACTGCAAAGGATTGAGCATGGCCAGTCCCTGCCGACGCTGGAGAAACTGGAGT
TTATCGCCCGGCGCTTGGATTTAAAAACGTCAGACCTTCTGGAGAGTGTTGATGTCCAATTGCCAGAGGATTATTGGGCT
ATGAAGGCTCGAATCGTTAAGTTCCCCACCTATGGGGAGAAAGAGCGGATTCAACGTAAGCTGGACCTGATTAACGAGGT
TTTGGTCAAGTACTTTGATCGTCTGCCTGAGGATGAGTTGCTCTTTTTGGACCTGTCAGAAAATATCTTGGATAGGATCC
ACGATAAACGTATCCCCAATATTGAGGAAATCTATGATGATGCTTTCGAGCAGGTGCTCAAGAAGAAAACCTTTACTTTT
AATGACTACCTTTATTTGAGTTATTTTTTACAAAAGTGTCGCAATGAAGCTAGTTATGATCGAAAGATCTTCAAACAGAT
TGAGCAAAAATTACTCCAACAAGAACTGAGTTCAGACGAGTTTTATAATATTGAATTTTTGGCAGCAGTTATGGACGCGG
CTGCTGTCTATGCTTTTCATGATGATTATAAGGATATCATGCCATTGTTGAAGAAAATACATTGGATTATTGAGCAAGCC
CAACTCCACACCTATAAGGCAGGGGTTCTAGAATTAGAAGCTAAATACTATGTTAAAATCGCTAAGGATAAGGATAGGGC
CAAGAAACTTTATCAACAAGCTCTAAAGTTAGGGGAAATCTTGGAGGATCCTGCTATTATTGCAGATATTAAGATGGAAA
TGAAAAACGATGGGATTGAGTAA

Domains

Predicted by InterproScan.

• Helix-turn-helix (HTH_3) (11-65)

• ComR tetratricopeptide (ComR_TPR) (75-294)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comR/comR1	Streptococcus sobrinus strain NIDR 6715-7	71.333	100	0.713
comR	Streptococcus salivarius strain HSISS4	42.475	100	0.425
comR	Streptococcus salivarius SK126	42.14	100	0.421
comR	Streptococcus thermophilus LMG 18311	42.14	100	0.421
comR	Streptococcus thermophilus LMD-9	42.14	100	0.421

References

• [1]: J W Li et al. (2021) A Novel Competence Pathway in the Oral Pathogen Streptococcus sobrinus. Journal of Dental Research 100(5):542-548. [PMID: 33876976]