Detailed information    

experimental Experimentally validated

Overview

Name   comR/comR1   Type   Regulator
Locus tag   DLJ51_RS00265 Genome accession   NZ_CP029560
Coordinates   38804..39709 (+) Length   301 a.a.
NCBI ID   WP_109982338.1    Uniprot ID   -
Organism   Streptococcus sobrinus strain NIDR 6715-7     
Function   repress natural transformation   
Competence regulation

Function

Transformation required at least 1 copy of comR2, and the transformation efficiency was inversely correlated with the number of copies of comR1.


Genomic Context

Location: 33804..44709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLJ51_RS00235 (DLJ51_00235) - 33839..33973 (+) 135 WP_255264604.1 ComC/BlpC family leader-containing pheromone/bacteriocin -
  DLJ51_RS10710 - 34003..34131 (+) 129 WP_019768929.1 hypothetical protein -
  DLJ51_RS10440 - 34186..34329 (+) 144 WP_019785178.1 hypothetical protein -
  DLJ51_RS00240 (DLJ51_00240) - 34591..35412 (+) 822 WP_019770819.1 ABC transporter ATP-binding protein -
  DLJ51_RS00245 (DLJ51_00245) - 35402..36121 (+) 720 WP_109833385.1 hypothetical protein -
  DLJ51_RS10715 - 36363..36491 (+) 129 WP_019768926.1 hypothetical protein -
  DLJ51_RS00250 (DLJ51_00250) - 36622..36852 (+) 231 WP_002959611.1 hypothetical protein -
  DLJ51_RS00255 (DLJ51_00255) - 37116..38453 (+) 1338 WP_019768925.1 hypothetical protein -
  DLJ51_RS00260 (DLJ51_00260) - 38479..38637 (+) 159 WP_019768924.1 bacteriocin -
  DLJ51_RS00265 (DLJ51_00265) comR/comR1 38804..39709 (+) 906 WP_109982338.1 XRE family transcriptional regulator Regulator
  - comS 39857..39922 (+) 66 - - Regulator
  DLJ51_RS00270 (DLJ51_00270) comA 39969..42086 (+) 2118 WP_019772678.1 peptide cleavage/export ABC transporter Regulator
  DLJ51_RS00275 (DLJ51_00275) comR/comR2 42279..43181 (+) 903 WP_109982339.1 XRE family transcriptional regulator Regulator
  DLJ51_RS00280 (DLJ51_00280) - 43311..44183 (-) 873 WP_109982340.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 301 a.a.        Molecular weight: 35541.62 Da        Isoelectric Point: 4.4380

>NTDB_id=579 DLJ51_RS00265 WP_109982338.1 38804..39709(+) (comR/comR1) [Streptococcus sobrinus strain NIDR 6715-7]
MTIKADLGRRIKAERNRKKISQSSFCGDEEKLTIRQLQRIEHGQSLPTLEKLEFIADRLDIKMSDLLEGRNIQLPDDYWE
MKARIVKFPTYGDEERIQQKQNLIDEIYDKYFDSLPEDELLFLDLSENILGGIHDKNIPDIEEIYDDAFDQVLKKERFDF
NDYLYLGYFLQQCRKDSDYDYKTFKKIEQKLLKQELLADDLYNIELLVTVMDAATVYAFHDDYGNIMPLLEKLNRIIDKA
QLPTYKVGLLELEAKYYIKVVEDKEKAKELYQRALMLGDILDDPTIIADVKMEMKNDGIDE

Nucleotide


Download         Length: 906 bp        

>NTDB_id=579 DLJ51_RS00265 WP_109982338.1 38804..39709(+) (comR/comR1) [Streptococcus sobrinus strain NIDR 6715-7]
ATGACAATAAAAGCAGATTTGGGACGACGGATTAAGGCCGAAAGAAACCGGAAAAAAATATCGCAGAGTTCATTTTGCGG
GGACGAAGAAAAGTTAACGATTCGTCAGTTGCAGCGGATTGAGCATGGCCAATCCCTACCAACTCTAGAAAAGTTGGAAT
TTATCGCTGATCGTTTGGACATTAAAATGTCTGACCTTCTGGAAGGTAGGAATATCCAATTACCGGATGACTATTGGGAA
ATGAAAGCACGGATTGTTAAATTTCCTACCTATGGAGATGAGGAAAGAATCCAGCAAAAACAGAATCTCATTGATGAAAT
TTATGATAAATACTTTGACAGTCTACCTGAGGATGAACTGCTCTTTTTGGACCTTTCAGAAAATATTCTAGGGGGTATTC
ATGACAAGAATATTCCTGATATTGAAGAAATTTATGATGATGCCTTCGATCAGGTTCTTAAAAAGGAGAGGTTTGATTTT
AACGATTACCTTTATTTGGGCTATTTTTTGCAACAGTGTCGCAAGGATTCCGATTATGATTATAAGACTTTTAAAAAAAT
TGAGCAGAAATTACTAAAGCAGGAGCTATTGGCAGATGATCTCTATAATATTGAACTTTTAGTGACAGTAATGGATGCTG
CTACTGTCTATGCTTTTCATGATGATTATGGAAATATCATGCCCTTGTTAGAAAAACTGAATCGTATTATTGATAAAGCT
CAACTCCCCACCTACAAGGTAGGACTTTTAGAACTTGAGGCCAAGTACTATATTAAGGTTGTTGAAGATAAAGAAAAAGC
CAAAGAACTTTACCAGCGCGCTCTGATGCTGGGAGATATTCTAGATGATCCCACCATTATTGCTGATGTTAAGATGGAAA
TGAAAAATGATGGGATAGATGAATAA

Domains

Predicted by InterproScan.

ComR tetratricopeptide (ComR_TPR)

(75-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comR/comR2 Streptococcus sobrinus strain NIDR 6715-7

71.333

100

0.713
  comR Streptococcus salivarius strain HSISS4

42.14

100

0.421
  comR Streptococcus thermophilus LMG 18311

42.14

100

0.421
  comR Streptococcus thermophilus LMD-9

41.472

100

0.415
  comR Streptococcus salivarius SK126

41.137

100

0.411

Multiple sequence alignment    



References

[1] J W Li et al. (2021) A Novel Competence Pathway in the Oral Pathogen Streptococcus sobrinus. Journal of Dental Research 100(5):542-548. [PMID: 33876976]