Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   STU_RS18145 Genome accession   NC_006448
Coordinates   1657567..1658667 (-) Length   366 a.a.
NCBI ID   WP_148148900.1    Uniprot ID   -
Organism   Streptococcus thermophilus LMG 18311     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1652567..1663667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STU_RS18105 (stu1856) - 1653179..1653376 (-) 198 WP_011681682.1 helix-turn-helix transcriptional regulator -
  STU_RS18110 (stu1857) - 1653625..1654818 (-) 1194 WP_022096898.1 acetate kinase -
  STU_RS18115 (stu1858) comYH 1654874..1655830 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  STU_RS18120 (stu1859) comGG 1655875..1656192 (-) 318 WP_011226623.1 competence type IV pilus minor pilin ComGG Machinery gene
  STU_RS18125 (stu1860) comGF 1656170..1656607 (-) 438 WP_014608742.1 competence type IV pilus minor pilin ComGF Machinery gene
  STU_RS18130 (stu1861) comGE 1656591..1656884 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE Machinery gene
  STU_RS18135 (stu1862) comGD 1656856..1657284 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  STU_RS18140 (stu1863) comGC 1657244..1657570 (-) 327 WP_011226627.1 competence type IV pilus major pilin ComGC Machinery gene
  STU_RS18145 (stu1864) comGB 1657567..1658667 (-) 1101 WP_148148900.1 competence type IV pilus assembly protein ComGB Machinery gene
  STU_RS18150 (stu1865) comGA 1658549..1659490 (-) 942 WP_011226629.1 competence type IV pilus ATPase ComGA Machinery gene
  STU_RS18155 (stu1866) - 1659571..1659933 (-) 363 WP_011226630.1 DUF1033 family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX comGB positive effect
  comX ssbA positive effect
  comX comGE positive effect
  comX comGD positive effect
  comX comGF positive effect
  comX comGG positive effect
  comX radA positive effect
  comX comEC positive effect
  comX comFC positive effect
  comX recA positive effect
  comX comEA positive effect
  comX comGC positive effect
  comX cinA positive effect
  comX coiA positive effect
  comX comGA positive effect
  comX comFA positive effect
  comX dprA positive effect
  clpC comX negative effect
  comR comX positive effect
  comR comS positive effect
  comS comX positive effect
  comS comS positive effect
  amiC comS positive effect
  eeP comS positive effect
  amiD comS positive effect
  amiF comS positive effect
  amiA3 comS positive effect
  amiE comS positive effect
  clpP comX negative effect
  mecA comX negative effect

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 41754.88 Da        Isoelectric Point: 10.0449

>NTDB_id=337 STU_RS18145 WP_148148900.1 1657567..1658667(-) (comGB) [Streptococcus thermophilus LMG 18311]
MPEKISKTIRLPTGISSWKVWLNKDVSLRGISKGKKLKISQQVKVIQLFKQLLKAGFTLTEIVAFLERSHLLKEASLSLM
KESLIRGDRLDQMLALVGFSDNIVTQISLADKHGNLLGSLTKIETYMLRMTKVRKKLMEVATYPILLLGFLILIMLGLKN
YLLPQLLEGDGKNNWAVQLVQIFPQLFFVSLCGLLVLGLILYLWVKRQSALVFYRRMAKIPFIGQTVRLYTTAYYAREWG
NLLGQGVDLLDLVALMQEQKSKLFRELGADLEEALMLGQSFPERIASHPFFTKELSLIIAYGEANARLGYELEVYAEEVW
QNFFNRLNKATTFVQPLIFVIVAVVIVMIYVAMLLPMYQNMEGMMS

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=337 STU_RS18145 WP_148148900.1 1657567..1658667(-) (comGB) [Streptococcus thermophilus LMG 18311]
TTGCCAGAGAAAATTTCCAAAACCATTCGTCTACCAACTGGAATCAGCAGTTGGAAGGTTTGGTTAAACAAGGATGTCTC
ACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAGTCAGCAAGTTAAGGTTATCCAGCTCTTCAAACAACTTTTAA
AGGCCGGTTTTACCTTAACTGAAATCGTAGCCTTTTTGGAGCGAAGTCACTTGCTGAAAGAAGCATCCTTGTCTCTTATG
AAAGAGAGTTTAATACGCGGTGATAGGTTGGATCAGATGTTAGCGTTAGTGGGGTTTTCGGACAATATTGTTACTCAGAT
TTCTCTTGCTGACAAGCACGGTAATCTTCTAGGGAGTCTAACAAAGATTGAAACCTATATGCTTCGTATGACAAAGGTTC
GCAAGAAACTCATGGAGGTGGCGACTTATCCCATACTACTTCTGGGTTTTCTGATTCTGATTATGCTAGGACTTAAAAAT
TATCTTCTACCCCAACTCTTAGAGGGTGATGGTAAGAATAATTGGGCTGTACAGTTGGTTCAAATTTTTCCCCAGCTTTT
TTTTGTGAGTTTGTGTGGACTCCTTGTGTTGGGTTTAATTCTCTATCTATGGGTGAAACGACAGTCAGCCCTTGTTTTTT
ATAGGCGAATGGCCAAAATCCCTTTTATTGGTCAGACAGTAAGGCTTTACACGACCGCCTATTATGCTAGGGAATGGGGA
AATTTATTAGGTCAAGGCGTTGATTTGCTAGATTTGGTGGCTCTTATGCAAGAGCAAAAGTCTAAACTCTTCCGTGAGCT
GGGAGCCGATTTGGAAGAAGCCCTGATGCTGGGACAGAGTTTTCCAGAACGTATTGCCAGTCATCCGTTTTTTACTAAGG
AGCTCTCACTAATTATTGCTTATGGGGAGGCCAATGCGAGGTTGGGATATGAGTTGGAAGTTTATGCCGAGGAGGTTTGG
CAAAACTTCTTTAACCGTCTTAATAAGGCAACAACCTTTGTGCAACCCCTCATTTTTGTTATTGTTGCAGTTGTGATTGT
AATGATCTATGTAGCCATGCTATTACCAATGTATCAAAATATGGAAGGAATGATGTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(236-356)

Type II secretion system (T2SS), protein F (T2SSF)

(51-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

54.678

93.443

0.511
  comYB Streptococcus mutans UA159

54.678

93.443

0.511
  comYB Streptococcus gordonii str. Challis substr. CH1

51.17

93.443

0.478
  comGB/cglB Streptococcus mitis NCTC 12261

50.453

90.437

0.456
  comGB/cglB Streptococcus mitis SK321

49.697

90.164

0.448
  comGB/cglB Streptococcus pneumoniae Rx1

49.394

90.164

0.445
  comGB/cglB Streptococcus pneumoniae D39

49.394

90.164

0.445
  comGB/cglB Streptococcus pneumoniae R6

49.394

90.164

0.445
  comGB/cglB Streptococcus pneumoniae TIGR4

49.394

90.164

0.445
  comGB Lactococcus lactis subsp. cremoris KW2

45.758

90.164

0.413

Multiple sequence alignment