Detailed information    

experimental Experimentally validated

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   SPR_RS09425 Genome accession   NC_003098
Coordinates   1833594..1834610 (-) Length   338 a.a.
NCBI ID   WP_074196785.1    Uniprot ID   -
Organism   Streptococcus pneumoniae R6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

ComGB is a critical component of the DNA uptake machinery during natural transformation. It is required for the assembly of the competence pseudopilus, a structure essential for DNA binding and transport across the cell membrane.


Genomic Context

Location: 1828594..1839610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS11020 - 1828671..1828802 (-) 132 WP_000768904.1 hypothetical protein -
  SPR_RS09385 (spr1854) - 1828803..1829993 (-) 1191 WP_000167757.1 acetate kinase -
  SPR_RS09390 (spr1855) comYH 1830044..1830997 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  SPR_RS09395 (spr1856) - 1831058..1831652 (-) 595 Protein_1829 class I SAM-dependent methyltransferase -
  SPR_RS09400 (spr1858) comGG/cglG 1831789..1832202 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  SPR_RS09405 (spr1859) comGF/cglF 1832180..1832641 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPR_RS09410 comGE/cglE 1832604..1832906 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SPR_RS09415 (spr1861) comGD/cglD 1832869..1833273 (-) 405 WP_000588026.1 competence type IV pilus minor pilin ComGD Machinery gene
  SPR_RS09420 (spr1862) comGC/cglC 1833266..1833592 (-) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  SPR_RS09425 (spr1863) comGB/cglB 1833594..1834610 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPR_RS09430 (spr1864) comGA/cglA/cilD 1834558..1835499 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  SPR_RS09435 (spr1865) - 1835575..1835940 (-) 366 WP_000286415.1 DUF1033 family protein -
  SPR_RS09440 (spr1866) - 1836091..1837149 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  SPR_RS09445 (spr1867) nagA 1837312..1838463 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38392.40 Da        Isoelectric Point: 9.4802

>NTDB_id=196 SPR_RS09425 WP_074196785.1 1833594..1834610(-) (comGB/cglB) [Streptococcus pneumoniae R6]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEV
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=196 SPR_RS09425 WP_074196785.1 1833594..1834610(-) (comGB/cglB) [Streptococcus pneumoniae R6]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAGTC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae TIGR4

100

100

1
  comGB/cglB Streptococcus pneumoniae Rx1

100

100

1
  comGB/cglB Streptococcus pneumoniae D39

100

100

1
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA159

55.952

97.959

0.548
  comYB Streptococcus mutans UA140

55.952

97.959

0.548
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506

Multiple sequence alignment    



References

[1] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]