Detailed information    

experimental Experimentally validated

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   SPR_RS09405 Genome accession   NC_003098
Coordinates   1832180..1832641 (-) Length   153 a.a.
NCBI ID   WP_000250534.1    Uniprot ID   -
Organism   Streptococcus pneumoniae R6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

ComGF is a component of the competence pseudopilus machinery.


Genomic Context

Location: 1827180..1837641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS09375 (spr1852) - 1827484..1828308 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  SPR_RS09380 (spr1853) rnpA 1828283..1828654 (-) 372 WP_000739246.1 ribonuclease P protein component -
  SPR_RS11020 - 1828671..1828802 (-) 132 WP_000768904.1 hypothetical protein -
  SPR_RS09385 (spr1854) - 1828803..1829993 (-) 1191 WP_000167757.1 acetate kinase -
  SPR_RS09390 (spr1855) comYH 1830044..1830997 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  SPR_RS09395 (spr1856) - 1831058..1831652 (-) 595 Protein_1829 class I SAM-dependent methyltransferase -
  SPR_RS09400 (spr1858) comGG/cglG 1831789..1832202 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  SPR_RS09405 (spr1859) comGF/cglF 1832180..1832641 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPR_RS09410 comGE/cglE 1832604..1832906 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SPR_RS09415 (spr1861) comGD/cglD 1832869..1833273 (-) 405 WP_000588026.1 competence type IV pilus minor pilin ComGD Machinery gene
  SPR_RS09420 (spr1862) comGC/cglC 1833266..1833592 (-) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  SPR_RS09425 (spr1863) comGB/cglB 1833594..1834610 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPR_RS09430 (spr1864) comGA/cglA/cilD 1834558..1835499 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  SPR_RS09435 (spr1865) - 1835575..1835940 (-) 366 WP_000286415.1 DUF1033 family protein -
  SPR_RS09440 (spr1866) - 1836091..1837149 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17972.67 Da        Isoelectric Point: 9.6092

>NTDB_id=200 SPR_RS09405 WP_000250534.1 1832180..1832641(-) (comGF/cglF) [Streptococcus pneumoniae R6]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=200 SPR_RS09405 WP_000250534.1 1832180..1832641(-) (comGF/cglF) [Streptococcus pneumoniae R6]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGTAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGATGATTTCCGTAAAACGAATGCTCG
TGGTCGAGGTTATCAGCCTATGGTTTATGGACTCAAATCTGTACGGATTACAGAGGACAATCAACTGGTTCGCTTTCATT
TCCAGTTCCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae TIGR4

100

100

1
  comGF/cglF Streptococcus pneumoniae D39

100

100

1
  comGF/cglF Streptococcus pneumoniae Rx1

100

100

1
  comGF/cglF Streptococcus mitis SK321

90.85

100

0.909
  comGF/cglF Streptococcus mitis NCTC 12261

89.542

100

0.895
  comGF Lactococcus lactis subsp. cremoris KW2

37.857

99.291

0.376
  comYF Streptococcus mutans UA140

40.299

93.056

0.375
  comYF Streptococcus mutans UA159

40.299

93.056

0.375

Multiple sequence alignment    



References

[1] Vitor Oliveira et al. (2021) The Role of Minor Pilins in Assembly and Function of the Competence Pilus of Streptococcus pneumoniae. Frontiers in Cellular And Infection Microbiology 11:808601. [PMID: 35004361]
[2] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]