Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   STU_RS11425 Genome accession   NC_006448
Coordinates   355380..356699 (+) Length   439 a.a.
NCBI ID   WP_011225516.1    Uniprot ID   Q5M5T4
Organism   Streptococcus thermophilus LMG 18311     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 350380..361699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STU_RS11395 (stu0362) - 351234..351998 (+) 765 WP_011225511.1 ABC transporter ATP-binding protein -
  STU_RS11400 (stu0363) - 351998..352708 (+) 711 WP_002947183.1 ABC transporter ATP-binding protein -
  STU_RS19130 - 352844..353504 (+) 661 Protein_319 CBS and ACT domain-containing protein -
  STU_RS11415 (stu0366) cysK 353671..354597 (-) 927 WP_002947179.1 cysteine synthase A -
  STU_RS11420 (stu0367) - 354699..355325 (-) 627 WP_011225515.1 YigZ family protein -
  STU_RS11425 (stu0368) comFA 355380..356699 (+) 1320 WP_011225516.1 DEAD/DEAH box helicase Machinery gene
  STU_RS11430 (stu0369) comFC 356680..357342 (+) 663 WP_002949747.1 ComF family protein Machinery gene
  STU_RS11435 (stu0370) hpf 357421..357969 (+) 549 WP_011225518.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX comFA positive effect
  comX ssbA positive effect
  comX comGE positive effect
  comX comGB positive effect
  comX comGD positive effect
  comX comGF positive effect
  comX comGG positive effect
  comX radA positive effect
  comX comEC positive effect
  comX comFC positive effect
  comX recA positive effect
  comX comEA positive effect
  comX comGC positive effect
  comX cinA positive effect
  comX coiA positive effect
  comX comGA positive effect
  comX dprA positive effect
  clpC comX negative effect
  comR comX positive effect
  comR comS positive effect
  comS comX positive effect
  comS comS positive effect
  amiC comS positive effect
  eeP comS positive effect
  amiD comS positive effect
  amiF comS positive effect
  amiA3 comS positive effect
  amiE comS positive effect
  clpP comX negative effect
  mecA comX negative effect

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50628.72 Da        Isoelectric Point: 9.5663

>NTDB_id=334 STU_RS11425 WP_011225516.1 355380..356699(+) (comFA) [Streptococcus thermophilus LMG 18311]
MIPKEYYGRLFTKEQLPVDYLSEAVKLESMIKVDKKLRCKRCYSRIEEDWQLPNGQYYCRACIVFGRNQEGKELYYFPSE
KSEVDFPVLKWSGKLTPYQNEVSEKLLKTYKNQKHSLVHAVTGAGKTEMIYNIVAYVLENKNRVVIASPRVDVCRELFLR
MQKDFTCSISLLHADSEPYDGSPLVIATTHQLLKFYHSFDLIIVDEVDAFPFVGNVMLNHAVKQAKTETGRYIYLTATST
LALEEQVRLGAIEKHHLASRFHGNPLVLPRFFWQGRLQKSLTSEKLPRPLIHQIKKQRKSNFPLLIFFPNIALGEKFSIT
LKKYLPTENIAFVSSKSEERSTIVEKFRKKELSILVTTTILERGVTFPQVDVFVCMANHYLYTSSSLIQIGGRVGRSPER
PTGKLYFFHEGLSKSMLQCREEINAMNKKGGFENEVSTM

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=334 STU_RS11425 WP_011225516.1 355380..356699(+) (comFA) [Streptococcus thermophilus LMG 18311]
ATGATACCTAAAGAATATTATGGACGACTATTTACGAAAGAACAGTTACCAGTGGATTATCTCTCAGAGGCTGTAAAATT
AGAAAGTATGATAAAGGTTGATAAAAAACTTAGATGTAAAAGATGTTATAGTCGAATAGAGGAAGATTGGCAATTACCGA
ATGGTCAGTATTATTGTAGAGCGTGTATTGTCTTTGGTCGAAACCAAGAAGGAAAAGAACTCTATTACTTTCCCTCAGAA
AAATCAGAAGTAGATTTTCCTGTCTTGAAATGGTCAGGAAAACTGACTCCTTATCAAAATGAGGTCTCGGAAAAGCTTTT
AAAGACTTATAAAAATCAAAAACACAGTCTTGTTCATGCAGTGACTGGTGCTGGCAAGACAGAGATGATTTATAATATTG
TTGCCTATGTTCTTGAAAATAAAAATCGTGTCGTCATCGCAAGTCCCCGAGTTGATGTTTGTCGAGAATTGTTTCTACGC
ATGCAGAAAGATTTTACTTGTAGTATTTCTCTGCTTCATGCTGATAGTGAACCATATGATGGTAGTCCGCTCGTTATAGC
TACCACTCATCAATTACTAAAATTTTATCATAGCTTTGACTTGATTATTGTTGACGAAGTTGATGCCTTTCCATTTGTAG
GGAATGTCATGTTAAATCATGCTGTTAAACAGGCAAAGACGGAAACAGGCCGGTATATTTACTTAACAGCAACTTCTACA
TTAGCTTTAGAAGAGCAAGTGCGCCTTGGAGCTATAGAAAAGCATCACCTTGCTAGTCGTTTCCACGGAAATCCTTTAGT
CCTTCCTCGTTTCTTTTGGCAAGGAAGGTTACAAAAGTCGTTGACGAGCGAGAAGCTTCCAAGGCCTCTAATTCACCAGA
TTAAGAAGCAGCGTAAATCAAATTTTCCTCTATTAATCTTTTTCCCCAATATAGCATTAGGTGAAAAGTTTAGTATTACC
CTAAAAAAATATCTCCCTACTGAAAACATAGCCTTTGTTTCATCAAAAAGCGAGGAGCGTTCAACCATCGTAGAGAAATT
CCGAAAAAAAGAATTGTCAATCTTAGTGACGACAACTATTCTCGAACGTGGTGTTACCTTTCCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATTACTTATATACTAGTTCGAGTCTTATTCAGATTGGTGGTAGGGTGGGGCGTTCGCCCGAGAGA
CCTACAGGGAAACTCTATTTCTTTCATGAAGGATTATCTAAATCAATGTTGCAATGTCGGGAAGAAATAAATGCAATGAA
TAAAAAAGGAGGGTTTGAAAATGAAGTGTCTACTATGTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(290-396)

Type III restriction enzyme, res subunit (ResIII)

(94-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M5T4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae D39

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae R6

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae TIGR4

54.988

98.178

0.54
  comFA/cflA Streptococcus mitis NCTC 12261

54.651

97.95

0.535
  comFA/cflA Streptococcus mitis SK321

53.721

97.95

0.526
  comFA Lactococcus lactis subsp. cremoris KW2

45.714

95.672

0.437
  comFA Latilactobacillus sakei subsp. sakei 23K

37.011

99.089

0.367

Multiple sequence alignment