Detailed information    

experimental Experimentally validated

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   SM12261_RS09240 Genome accession   NZ_CP028414
Coordinates   1836472..1837770 (-) Length   432 a.a.
NCBI ID   WP_000867722.1    Uniprot ID   A0AAX2L3B0
Organism   Streptococcus mitis NCTC 12261     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 1831472..1842770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS09220 (SM12261_1778) rplI 1832627..1833079 (-) 453 WP_000864218.1 50S ribosomal protein L9 -
  SM12261_RS09225 (SM12261_1779) - 1833076..1835049 (-) 1974 WP_000715136.1 DHH family phosphoesterase -
  SM12261_RS09230 (SM12261_1780) hpf 1835185..1835733 (-) 549 WP_000599102.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SM12261_RS09235 (SM12261_1781) comFC/cflB 1835813..1836475 (-) 663 WP_000649953.1 ComF family protein Machinery gene
  SM12261_RS09240 (SM12261_1782) comFA/cflA 1836472..1837770 (-) 1299 WP_000867722.1 DEAD/DEAH box helicase Machinery gene
  SM12261_RS09245 (SM12261_1783) - 1837827..1838462 (+) 636 WP_000395748.1 YigZ family protein -
  SM12261_RS09250 (SM12261_1784) - 1838478..1838921 (+) 444 WP_000863906.1 PH domain-containing protein -
  SM12261_RS09255 (SM12261_1785) cysK 1839018..1839944 (+) 927 WP_000029880.1 cysteine synthase A -
  SM12261_RS09260 (SM12261_1786) tsf 1840066..1841106 (-) 1041 WP_000808076.1 translation elongation factor Ts -
  SM12261_RS09265 (SM12261_1787) rpsB 1841185..1841964 (-) 780 WP_000268459.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49667.61 Da        Isoelectric Point: 8.6690

>NTDB_id=507 SM12261_RS09240 WP_000867722.1 1836472..1837770(-) (comFA/cflA) [Streptococcus mitis NCTC 12261]
MKVNPNYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGTYYCRECLLMKRVRSDQSLYYFPQ
EDFPKQDVLKWRGQLTPFQDKVSQGLLQAVDKQEPSLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQKDFACEIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
DELDRKVRIGELKRLSLPRRFHGNPLIIPKPVWLSDFNHCLEKSRLSPKLKSYIEKQRKTGYPLLIFASEIKKGEQLKEI
IQEQFPNEKIGFVSSITENRLEQVQAFRDRELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNTSIKKAIKEIQQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=507 SM12261_RS09240 WP_000867722.1 1836472..1837770(-) (comFA/cflA) [Streptococcus mitis NCTC 12261]
ATGAAAGTAAATCCAAATTATCTCGGTCGCTTGTTTACTGAGAATGAATTAACAGAAGAGGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAAGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTACTTACTATTGTAGGGAGTGTTTACTGATGAAGCGAGTCAGGAGTGATCAATCTTTATACTATTTCCCACAG
GAGGATTTTCCGAAGCAAGATGTTCTTAAATGGCGTGGTCAATTAACCCCTTTTCAAGATAAGGTATCACAGGGACTGCT
TCAAGCGGTAGACAAGCAAGAGCCAAGCTTGGTTCACGCTGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TAGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCCAGTCCTCGCATAGACGTTTGTTTGGAGCTGTATAAGCGC
CTGCAAAAGGATTTTGCTTGTGAGATAGCCCTCCTACATGGAGAATCAGAGCCGTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTACTGATAGTGGATGAAGTAGATGCCTTTCCTTATGTTG
ACAATCCTATGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGCTTGAGAATCTTTTTAACAGCAACTTCTACC
GATGAGTTAGATAGGAAGGTTCGCATCGGAGAATTAAAACGATTGAGTTTGCCAAGACGATTTCATGGAAATCCTTTGAT
TATTCCTAAACCTGTCTGGTTATCCGATTTTAATCACTGTTTAGAGAAAAGTCGGTTGTCACCAAAGCTAAAGTCTTATA
TTGAGAAGCAGAGAAAGACAGGTTATCCGTTACTCATTTTTGCTTCAGAGATTAAGAAAGGGGAACAGTTAAAAGAAATC
ATACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTATCACAGAAAATCGATTAGAGCAGGTACAAGCTTT
TCGAGATAGAGAACTGACAATACTTATCAGTACGACGATATTGGAGCGTGGAGTTACCTTCCCTTGTGTTGACGTTTTCG
TAGTAGAGGCCAATCATCGTCTGTTTACCAAGTCTAGTTTAATACAGATTGGAGGACGTGTCGGTCGGAGTATGGACAGA
CCGACAGGAGATTTGCTTTTCTTCCATGACGGGTTAAATACTTCAATCAAAAAGGCAATTAAGGAAATTCAACAGATGAA
TAAGGAGGCGGGTCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(292-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis SK321

93.75

100

0.938
  comFA/cflA Streptococcus pneumoniae R6

93.519

100

0.935
  comFA/cflA Streptococcus pneumoniae D39

93.519

100

0.935
  comFA/cflA Streptococcus pneumoniae Rx1

93.519

100

0.935
  comFA/cflA Streptococcus pneumoniae TIGR4

93.287

100

0.933
  comFA Lactococcus lactis subsp. cremoris KW2

49.772

100

0.507
  comFA Latilactobacillus sakei subsp. sakei 23K

38.515

99.769

0.384

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]