Detailed information    

experimental Experimentally validated

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   SMSK321_RS10515 Genome accession   NZ_AEDT01000032
Coordinates   206342..207640 (-) Length   432 a.a.
NCBI ID   WP_000867726.1    Uniprot ID   -
Organism   Streptococcus mitis SK321     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 201342..212640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS0102860 rplI 202497..202949 (-) 453 WP_000864214.1 50S ribosomal protein L9 -
  SMSK321_RS10505 (SMSK321_1612) - 202946..204919 (-) 1974 WP_000715142.1 DHH family phosphoesterase -
  SMSK321_RS10510 (SMSK321_1613) hpf 205055..205603 (-) 549 WP_000599100.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SMSK321_RS0102865 comFC/cflB 205683..206345 (-) 663 WP_000649958.1 ComF family protein Machinery gene
  SMSK321_RS10515 (SMSK321_1614) comFA/cflA 206342..207640 (-) 1299 WP_000867726.1 DEAD/DEAH box helicase Machinery gene
  SMSK321_RS10520 (SMSK321_1615) - 207697..208332 (+) 636 WP_000395766.1 YigZ family protein -
  SMSK321_RS12680 - 208426..208521 (+) 96 Protein_211 PH domain-containing protein -
  SMSK321_RS10525 (SMSK321_1616) cysK 208618..209544 (+) 927 WP_000029882.1 cysteine synthase A -
  SMSK321_RS10530 (SMSK321_1617) tsf 209666..210706 (-) 1041 WP_000808070.1 translation elongation factor Ts -
  SMSK321_RS10535 (SMSK321_1618) rpsB 210785..211564 (-) 780 WP_000268469.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49657.69 Da        Isoelectric Point: 8.3631

>NTDB_id=535 SMSK321_RS10515 WP_000867726.1 206342..207640(-) (comFA/cflA) [Streptococcus mitis SK321]
MKVNPNYLGRLFTENELTKEERQLAEKLPAMRKEKGKLFCQRCDSAILDEWYLPIGAYYCRECLLMKRVRSDQVLYYFPQ
EDFAKQDILKWRGQLTPFQEKVSEGLIQAVDKQEPILVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQDDFACEISLLYGESEPYFRTPLVVSTTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
DELDRKVRIGELKRLSLPRRFHGNPLIIPKPVWLSDFNRYLEKKCLSPKLKSYIEKQRKTGYPLLIFASEIKKGEQLKEI
LQKEFPHEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNTSIKKAIKEIQQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=535 SMSK321_RS10515 WP_000867726.1 206342..207640(-) (comFA/cflA) [Streptococcus mitis SK321]
ATGAAAGTAAATCCAAATTATCTCGGTCGGTTGTTTACGGAGAATGAATTAACTAAAGAGGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAAGGGAAACTTTTCTGTCAACGTTGTGATAGTGCTATTTTAGATGAATGGTATTTGC
CCATCGGTGCTTACTATTGTAGGGAGTGTTTGCTGATGAAGCGAGTCAGGAGTGATCAAGTTTTATACTATTTTCCGCAG
GAGGATTTTGCGAAGCAAGATATTCTTAAATGGCGTGGTCAATTAACTCCTTTTCAAGAGAAGGTATCAGAGGGACTAAT
TCAAGCTGTAGACAAGCAGGAACCAATCTTGGTTCACGCTGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TAGCTAAAGTGATCAATGCGGGTGGTGCAGTATGCTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAAGATGATTTTGCTTGCGAGATATCTTTACTATACGGAGAATCAGAGCCGTATTTTCGAACACCACTAGTTGTTTC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCCTTTCCTTATGTTG
ATAATCCCATGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGCTTGAGAATCTTTTTAACAGCAACTTCGACT
GATGAGTTAGATAGGAAGGTTCGCATAGGAGAACTAAAACGATTGAGTTTGCCAAGACGATTTCATGGAAATCCGTTGAT
TATTCCTAAACCTGTCTGGTTATCCGATTTTAATCGCTATTTAGAGAAGAAGTGTTTGTCACCAAAGTTAAAGTCTTATA
TTGAGAAGCAGAGAAAGACAGGTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAAAAGAAATC
TTACAGAAGGAATTTCCGCATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAGGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTGATCAGTACGACGATATTGGAGCGTGGAGTTACTTTCCCTTGTGTGGACGTTTTCG
TAGTAGAGGCCAATCATCGTCTTTTTACCAAGTCTAGTTTAATACAGATTGGAGGACGTGTCGGTCGGAGTATGGACAGA
CCGACAGGAGATTTGCTTTTTTTCCATGATGGGTTAAATACTTCAATCAAGAAGGCAATTAAGGAAATTCAGCAGATGAA
TAAGGAGGCGGGGCTATGA

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

93.75

100

0.938
  comFA/cflA Streptococcus pneumoniae D39

92.361

100

0.924
  comFA/cflA Streptococcus pneumoniae Rx1

92.361

100

0.924
  comFA/cflA Streptococcus pneumoniae R6

92.361

100

0.924
  comFA/cflA Streptococcus pneumoniae TIGR4

92.13

100

0.921
  comFA Lactococcus lactis subsp. cremoris KW2

50.228

100

0.512
  comFA Latilactobacillus sakei subsp. sakei 23K

38.621

100

0.389
  comFA Bacillus subtilis subsp. subtilis str. 168

39.303

93.056

0.366

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]