Detailed information NTG0000108

Experimentally validated

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: BSU_35470
• Genome accession: NC_000964
• Coordinates: 3642167..3643558 (-)
• Length: 463 a.a.
• NCBI ID: NP_391427.1
• Uniprot ID: P39145
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: ssDNA transport into the cell DNA binding and uptake

Function

ComFA is a membrane-associated ATPase that provides the energy for the transport of single-stranded DNA across the cytoplasmic membrane.

Genomic Context

Location: 3637167..3648558

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSU_35400 (BSU35400)	flgL	3637338..3638234 (-)	897	NP_391420.1	flagellar hook-filament junction protein	-
BSU_35410 (BSU35410)	flgK	3638245..3639768 (-)	1524	NP_391421.1	flagellar hook-filament junction	-
BSU_35420 (BSU35420)	flgN	3639787..3640269 (-)	483	NP_391422.1	factor required for flagellar based motility	-
BSU_35430 (BSU35430)	flgM	3640285..3640551 (-)	267	NP_391423.1	anti-sigma factor repressor of sigma(D)-dependent transcription	-
BSU_35440 (BSU35440)	yvyF	3640632..3641051 (-)	420	NP_391424.1	putative transcriptional regulator of flagella formation	-
BSU_35450 (BSU35450)	comFC	3641125..3641847 (-)	723	NP_391425.2	component of the DNA transport apparatus	Machinery gene
BSU_35460 (BSU35460)	comFB	3641811..3642107 (-)	297	NP_391426.1	regulator of competence, pole located	-
BSU_35470 (BSU35470)	comFA	3642167..3643558 (-)	1392	NP_391427.1	ATP-dependent helicase competence protein	Machinery gene
BSU_35480 (BSU35480)	fakBA	3643664..3644509 (-)	846	NP_391428.1	fatty acid kinase fatty acid binding subunit A	-
BSU_35490 (BSU35490)	degU	3644607..3645296 (-)	690	NP_391429.1	two-component response regulator	Regulator
BSU_35500 (BSU35500)	degS	3645379..3646536 (-)	1158	NP_391430.1	two-component sensor histidine kinase [DegU]	Regulator
BSU_35510 (BSU35510)	yvyE	3646753..3647406 (+)	654	NP_391431.1	putative translation regulator	-

Sequence

Protein

• Length: 463 a.a.
• Molecular weight: 52565.63 Da
• Isoelectric Point: 10.0082

>NTDB_id=108 BSU_35470 NP_391427.1 3642167..3643558(-) (comFA) [Bacillus subtilis subsp. subtilis str. 168]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide

• Length: 1392 bp

>NTDB_id=108 BSU_35470 NP_391427.1 3642167..3643558(-) (comFA) [Bacillus subtilis subsp. subtilis str. 168]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (326-423)

• Type III restriction enzyme, res subunit (ResIII) (120-268)

3D structure

• AlphaFold DB: P39145

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	36.08	100	0.377
comFA/cflA	Streptococcus mitis SK321	39.303	93.056	0.366
comFA	Latilactobacillus sakei subsp. sakei 23K	39.268	92.135	0.362

References

• [1]: Scott S Chilton et al. (2017) A Conserved Metal Binding Motif in the Bacillus subtilis Competence Protein ComFA Enhances Transformation. Journal of Bacteriology 199(15):e00272-17. [PMID: 28559293]
• [2]: Masaomi Takeno et al. (2011) Role of ComFA in controlling the DNA uptake rate during transformation of competent Bacillus subtilis. Journal of Bioscience And Bioengineering 111(6):618-23. [PMID: 21397556]
• [3]: Jeanette Hahn et al. (2005) Transformation proteins and DNA uptake localize to the cell poles in Bacillus subtilis. Cell 122(1):59-71. [PMID: 16009133]
• [4]: Mathieu Bergé et al. (2002) Uptake of transforming DNA in Gram-positive bacteria: a view from Streptococcus pneumoniae. Molecular Microbiology 45(2):411-21. [PMID: 12123453]
• [5]: J A Londoño-Vallejo et al. (1994) Mutation of the putative nucleotide binding site of the Bacillus subtilis membrane protein ComFA abolishes the uptake of DNA during transformation. Journal of Bacteriology 176(15):4642-5. [PMID: 8045895]
• [6]: J A Londoño-Vallejo et al. (1993) comF, a Bacillus subtilis late competence locus, encodes a protein similar to ATP-dependent RNA/DNA helicases. Molecular Microbiology 9(1):119-31. [PMID: 8412657]