Detailed information NTG0000130

Experimentally validated

Overview

• Name: degS
• Type: Regulator
• Locus tag: BSU_35500
• Genome accession: NC_000964
• Coordinates: 3645379..3646536 (-)
• Length: 385 a.a.
• NCBI ID: NP_391430.1
• Uniprot ID: P13799
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: phosphorylation of DegU Competence regulation

Function

The degS gene is part of the DegS-DegU two-component system. DegS is a histidine kinase that phosphorylates DegU, which in turn regulates the expression of competence-related genes.

Genomic Context

Location: 3640379..3651536

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSU_35440 (BSU35440)	yvyF	3640632..3641051 (-)	420	NP_391424.1	putative transcriptional regulator of flagella formation	-
BSU_35450 (BSU35450)	comFC	3641125..3641847 (-)	723	NP_391425.2	component of the DNA transport apparatus	Machinery gene
BSU_35460 (BSU35460)	comFB	3641811..3642107 (-)	297	NP_391426.1	regulator of competence, pole located	-
BSU_35470 (BSU35470)	comFA	3642167..3643558 (-)	1392	NP_391427.1	ATP-dependent helicase competence protein	Machinery gene
BSU_35480 (BSU35480)	fakBA	3643664..3644509 (-)	846	NP_391428.1	fatty acid kinase fatty acid binding subunit A	-
BSU_35490 (BSU35490)	degU	3644607..3645296 (-)	690	NP_391429.1	two-component response regulator	Regulator
BSU_35500 (BSU35500)	degS	3645379..3646536 (-)	1158	NP_391430.1	two-component sensor histidine kinase [DegU]	Regulator
BSU_35510 (BSU35510)	yvyE	3646753..3647406 (+)	654	NP_391431.1	putative translation regulator	-
BSU_35520 (BSU35520)	tagV	3647406..3648581 (+)	1176	NP_391432.1	teichoic acid-peptidoglycan tethering enzyme (LCP component) with transcription regulator domain	-
BSU_35530 (BSU35530)	tagO	3648654..3649730 (-)	1077	NP_391433.1	UDP-N-acetylglucosamine:undecaprenyl-P N-acetylglucosaminyl-1-P transferase	-
BSU_35540 (BSU35540)	tuaH	3649875..3651068 (-)	1194	NP_391434.1	putative glycosyltransferase (teichuronic acid)	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
degS	degU	positive effect
degU	comK	positive effect
comK	comK	positive effect
comK	late competence genes	positive effect
codY	comK	negative effect
clpP	comK	negative effect
mecA	comK	negative effect
kre	comK	negative effect
abrB	comK	negative effect
comS	comK	positive effect
spo0A	comK	positive effect
rok	comK	negative effect
clpC	comK	negative effect
med	comK	positive effect
spo0A	comK	negative effect
comK	late competence genes	positive effect
clpP	degU	negative effect
abrB	rok	negative effect
spo0A	abrB	negative effect
comA	comS	positive effect
spo0A	rok	negative effect
sinR	spo0A	negative effect
sinR	rok	negative effect
sinR	degU	negative effect
degQ	degU	positive effect
clpC	degU	negative effect
rapC	comA	negative effect
rapF	comA	negative effect
comP	comA	positive effect
sinI	sinR	negative effect
phrC	rapC	negative effect
phrF	rapF	negative effect
comX	comP	positive effect
comQ	comX	positive effect

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 44957.76 Da
• Isoelectric Point: 6.2957

>NTDB_id=130 BSU_35500 NP_391430.1 3645379..3646536(-) (degS) [Bacillus subtilis subsp. subtilis str. 168]
MNKTKMDSKVLDSILMKMLKTVDGSKDEVFQIGEQSRQQYEQLVEELKQIKQQVYEVIELGDKLEVQTRHARNRLSEVSR
NFHRFSEEEIRNAYEKAHKLQVELTMIQQREKQLRERRDDLERRLLGLQEIIERSESLVSQITVVLNYLNQDLREVGLLL
ADAQAKQDFGLRIIEAQEEERKRVSREIHDGPAQMLANVMMRSELIERIFRDRGAEDGFQEIKNLRQNVRNALYEVRRII
YDLRPMALDDLGLIPTLRKYLYTTEEYNGKVKIHFQCIGETEDQRLAPQFEVALFRLAQEAVSNALKHSESEEITVKVEI
TKDFVILMIKDNGKGFDLKEAKEKKNKSFGLLGMKERVDLLEGTMTIDSKIGLGTFIMIKVPLSL

Nucleotide

• Length: 1158 bp

>NTDB_id=130 BSU_35500 NP_391430.1 3645379..3646536(-) (degS) [Bacillus subtilis subsp. subtilis str. 168]
ATGAATAAAACAAAGATGGATTCCAAAGTGCTGGATTCTATTTTGATGAAGATGCTGAAAACCGTTGACGGGAGCAAGGA
CGAGGTTTTTCAAATCGGGGAGCAGTCACGCCAGCAGTATGAACAGCTGGTCGAAGAACTGAAACAAATTAAACAGCAGG
TGTATGAAGTGATTGAGCTTGGCGATAAACTTGAAGTGCAAACTCGCCATGCGAGAAACCGTTTATCCGAGGTCAGCCGT
AATTTTCATAGATTCAGTGAAGAGGAAATCCGCAATGCTTATGAAAAAGCCCATAAGCTGCAGGTAGAATTGACGATGAT
CCAGCAGCGTGAGAAGCAATTGCGCGAACGGCGGGACGATTTGGAGCGCAGATTGCTAGGGCTTCAGGAAATCATTGAGC
GGTCAGAATCATTAGTAAGCCAAATTACAGTTGTGCTCAACTACTTGAATCAGGATTTGCGCGAAGTTGGACTGCTTCTT
GCTGATGCTCAGGCAAAACAGGATTTCGGCTTAAGAATTATTGAGGCGCAGGAAGAAGAGCGAAAAAGAGTCTCAAGAGA
AATCCATGACGGACCCGCTCAAATGCTGGCGAATGTTATGATGAGATCGGAATTAATCGAGCGGATTTTCCGTGACCGGG
GCGCAGAGGACGGATTCCAAGAAATTAAAAATCTCCGCCAAAATGTTCGGAATGCCCTTTACGAAGTGAGAAGGATTATA
TATGATTTAAGACCGATGGCCCTTGATGACCTAGGCCTGATTCCAACTTTAAGAAAATATCTATATACAACCGAGGAATA
TAACGGGAAGGTCAAAATACATTTTCAGTGCATTGGAGAAACAGAGGATCAGAGGCTAGCGCCTCAGTTTGAGGTTGCGC
TCTTCAGGCTCGCACAGGAAGCTGTGTCTAATGCGCTAAAGCATTCTGAATCTGAAGAAATTACAGTCAAAGTTGAGATC
ACAAAGGATTTTGTGATTTTAATGATAAAAGATAACGGTAAAGGGTTCGACCTGAAGGAAGCGAAAGAGAAGAAAAACAA
ATCATTCGGCTTGCTGGGCATGAAAGAAAGAGTAGATTTATTGGAAGGAACGATGACAATAGATTCGAAAATAGGTCTTG
GGACATTTATTATGATTAAGGTTCCGTTATCTCTTTGA

Domains

Predicted by InterproScan.

• Histidine kinase (HisKA_3) (180-248)

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (291-384)

• Sensor protein DegS (DegS) (12-170)

3D structure

• AlphaFold DB: P13799

References

• [1]: Mathieu Miras et al. (2016) A DegU-P and DegQ-Dependent Regulatory Pathway for the K-state in Bacillus subtilis. Frontiers in Microbiology 7:1868. [PMID: 27920766]
• [2]: F Kunst et al. (1994) The DegS/DegU and ComP/ComA two-component systems are part of a network controlling degradative enzyme synthesis and competence in Bacillus subtilis. Research in Microbiology 145(5-6):393-402. [PMID: 7855425]
• [3]: T Msadek et al. (1991) DegS-DegU and ComP-ComA modulator-effector pairs control expression of the Bacillus subtilis pleiotropic regulatory gene degQ. Journal of Bacteriology 173(7):2366-77. [PMID: 1901055]