Detailed information NTG0000091

Experimentally validated

Overview

• Name: comP
• Type: Regulator
• Locus tag: BSU_31690
• Genome accession: NC_000964
• Coordinates: 3253529..3255838 (-)
• Length: 769 a.a.
• NCBI ID: NP_391047.2
• Uniprot ID: Q99027
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: phosphorylation of ComA Competence regulation

Function

ComP senses environmental signals and phosphorylates its cognate response regulator, ComA, which in turn activates the expression of downstream genes required for competence.

Genomic Context

Location: 3248529..3260838

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSU_31610 (BSU31610)	mrpB	3248996..3249427 (+)	432	NP_391039.1	Na+/H+ antiporter complex	-
BSU_31620 (BSU31620)	mrpC	3249427..3249768 (+)	342	NP_391040.2	component of Na+/H+ antiporter	-
BSU_31630 (BSU31630)	mrpD	3249761..3251242 (+)	1482	NP_391041.3	proton transporter component of Na+/H+ antiporter	-
BSU_31640 (BSU31640)	mrpE	3251248..3251724 (+)	477	YP_054591.2	non essential component of Na+/H+ antiporter	-
BSU_31650 (BSU31650)	mrpF	3251724..3252008 (+)	285	NP_391043.2	efflux transporter for Na+ and cholate	-
BSU_31660 (BSU31660)	mrpG	3251992..3252366 (+)	375	NP_391044.1	non essential component of Na+/H+ antiporter	-
BSU_31670 (BSU31670)	yuxO	3252405..3252785 (-)	381	NP_391045.2	putative proofreading thioesterase in bacillibactin biosynthesis	-
BSU_31680 (BSU31680)	comA	3252804..3253448 (-)	645	NP_391046.1	two-component response quorum-sensing regulator	Regulator
BSU_31690 (BSU31690)	comP	3253529..3255838 (-)	2310	NP_391047.2	two-component sensor histidine kinase	Regulator
BSU_31700 (BSU31700)	comX	3255853..3256020 (-)	168	NP_391048.1	competence pheromone precursor (pheromone peptide aa 46->55, geranyl-modified)	Regulator
BSU_31710 (BSU31710)	comQ	3256008..3256907 (-)	900	NP_391049.2	isoprenyl transferase (pre-ComX modification)	Regulator
BSU_31720 (BSU31720)	degQ	3257092..3257232 (-)	141	NP_391050.1	pleiotropic regulator	Regulator
BSU_31725 (BSU31725)	-	3257454..3257579 (+)	126	YP_009514001.1	hypothetical protein	-
BSU_31730 (BSU31730)	cotIC	3257693..3258061 (+)	369	NP_391051.2	inner spore coat protein	-
BSU_31740 (BSU31740)	pdeH	3258037..3259266 (-)	1230	NP_391052.1	cyclic di-GMP phosphodiesterase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comP	comA	positive effect
comA	comS	positive effect
comS	comK	positive effect
comK	comK	positive effect
comK	late competence genes	positive effect
codY	comK	negative effect
clpP	comK	negative effect
mecA	comK	negative effect
kre	comK	negative effect
abrB	comK	negative effect
spo0A	comK	positive effect
rok	comK	negative effect
degU	comK	positive effect
clpC	comK	negative effect
med	comK	positive effect
spo0A	comK	negative effect
comK	late competence genes	positive effect
clpP	degU	negative effect
abrB	rok	negative effect
spo0A	abrB	negative effect
spo0A	rok	negative effect
sinR	spo0A	negative effect
sinR	rok	negative effect
sinR	degU	negative effect
degQ	degU	positive effect
clpC	degU	negative effect
degS	degU	positive effect
rapC	comA	negative effect
rapF	comA	negative effect
sinI	sinR	negative effect
phrC	rapC	negative effect
phrF	rapF	negative effect
comX	comP	positive effect
comQ	comX	positive effect

Sequence

Protein

• Length: 769 a.a.
• Molecular weight: 89308.77 Da
• Isoelectric Point: 8.2618

>NTDB_id=91 BSU_31690 NP_391047.2 3253529..3255838(-) (comP) [Bacillus subtilis subsp. subtilis str. 168]
MKNLIKKFTIAVIVLSILYISYTTYISMNGIIIGTKIHKNDKSQFMIEEISESSYGQFVGLRQGDIILKINKEKPSDKHL
KWGYLSHINSLDILRSGKKIHLKDFDLVTLNRPYSFFLFVLPLFFYFLSIICIFYILKVNKKRRSFAAYILILLLLDISI
AYISAGGPFRGHIINRYINLFTFISSPILYLQFIQRYLGEIGKTFLNRISFLYIIPIFNLGIEFFQDYLQVDIDFLATLN
LVSFATLTLFSFSAIYLHLNKYKYAEHSFILKLLILTNTLSFAPFLIFFVLPIIFTGNYIFPALASASLLVLIPFGLVYQ
FVANKMFDIEFILGRMRYYALLAMIPTLLIVGALVLFDVMDIQMNPVRQTVFFFVVMFAVFYFKEVMDFKFRLKRFSEKF
NYQDSIFKYTQLMRGVTSLQQVFKELKNTILDVLLVSKAYTFEVTPDHKVIFLDKHEVGPDWNFYQEEFENVTSEIGKII
EVNQGFLMKVGERGGSSYVLLCLSNINTPRLTRDEISWLKTLSFYTSVSMENVLHIEELMEHLKDLKQEGTNPIWLKKLM
FAIEEKQRSGLARDLHDSVLQDLISLKRQCELFLGDFKKDDNPCREEVQDKLVQMNEQMSDVISMTRETCHELRPQLLYD
LGLVKALSKLVAQQQERVPFHIRLNTGRFTASLDLDSQLNLYRIIQEFLSNAVKHSQATDVLIMLISIQNKIVLHYEDDG
VGFDQEKNTEHSMSMGLSGIKERVRALDGRLRIETSEGKGFKADIEIEL

Nucleotide

• Length: 2310 bp

>NTDB_id=91 BSU_31690 NP_391047.2 3253529..3255838(-) (comP) [Bacillus subtilis subsp. subtilis str. 168]
TTGAAGAACTTAATAAAAAAATTCACAATAGCTGTAATTGTTTTGAGTATCCTTTATATAAGCTATACAACTTATATCAG
TATGAATGGAATTATTATTGGGACTAAGATTCATAAAAATGATAAAAGTCAATTTATGATAGAAGAAATATCGGAATCTT
CATATGGACAATTTGTTGGTCTGAGACAGGGAGACATCATATTAAAAATTAATAAAGAAAAACCTTCAGACAAACATTTG
AAATGGGGATATTTGAGTCATATTAACAGTCTGGATATTTTGAGAAGTGGAAAAAAGATTCATCTCAAAGATTTTGATTT
AGTTACTCTAAATAGACCTTATAGTTTTTTTCTGTTCGTACTTCCTTTGTTTTTTTATTTTTTAAGCATAATATGTATTT
TTTATATACTTAAAGTAAATAAAAAAAGGAGATCATTTGCTGCCTATATTCTTATTCTATTGTTATTAGACATTTCGATA
GCATATATAAGTGCAGGCGGACCATTTAGAGGACATATAATCAATCGGTATATTAACTTGTTTACTTTTATATCTTCTCC
TATTCTTTATCTTCAATTTATACAAAGATACCTTGGAGAAATAGGTAAAACTTTTTTGAATAGAATTTCTTTTCTTTATA
TCATACCAATATTTAATCTTGGTATTGAGTTTTTTCAAGACTATTTACAAGTAGATATTGATTTTTTAGCAACTCTTAAT
TTGGTTTCTTTTGCAACTTTGACTCTATTTTCTTTTTCAGCGATATACTTACATCTTAATAAATATAAATATGCTGAGCA
TTCGTTTATTCTTAAATTACTAATTTTAACAAACACTCTTTCATTTGCGCCTTTTTTGATATTTTTTGTTCTTCCAATAA
TATTTACAGGTAATTATATTTTCCCGGCATTAGCTTCGGCGTCATTACTAGTACTAATTCCGTTCGGGTTAGTATACCAA
TTTGTAGCCAATAAGATGTTTGATATAGAGTTTATCTTAGGAAGAATGAGATACTATGCTCTACTTGCCATGATACCAAC
TCTTCTAATAGTTGGTGCATTAGTTCTGTTTGATGTAATGGACATCCAGATGAACCCTGTGCGTCAAACTGTATTTTTTT
TCGTTGTCATGTTCGCTGTCTTTTATTTTAAAGAGGTCATGGATTTTAAATTTCGGTTAAAACGTTTCTCCGAAAAATTC
AACTATCAGGACAGTATTTTTAAATATACTCAGCTAATGAGGGGTGTAACTTCTCTTCAACAAGTTTTTAAAGAACTGAA
AAATACTATACTGGATGTTTTGCTTGTAAGCAAAGCTTATACCTTTGAGGTTACTCCTGATCACAAAGTGATATTTTTAG
ATAAGCATGAAGTTGGACCGGACTGGAATTTTTATCAAGAGGAATTTGAAAACGTAACTTCAGAAATTGGGAAAATTATA
GAAGTCAATCAAGGCTTTCTTATGAAAGTTGGTGAACGAGGCGGTAGTTCTTATGTTCTGCTTTGTTTATCTAATATTAA
CACTCCCCGGCTAACACGTGATGAAATATCGTGGCTGAAAACACTGTCTTTTTATACAAGTGTGTCCATGGAAAATGTCC
TGCATATTGAGGAGCTCATGGAACATTTGAAGGACTTAAAACAAGAGGGAACCAACCCCATCTGGCTGAAAAAGCTAATG
TTTGCAATCGAAGAAAAACAGCGTTCAGGACTCGCCCGCGATCTCCACGATTCGGTTCTTCAGGATTTGATTTCCTTAAA
ACGCCAGTGTGAGCTGTTTTTGGGTGATTTCAAGAAGGATGATAATCCGTGCCGTGAAGAGGTGCAGGACAAGCTTGTAC
AGATGAATGAGCAGATGTCTGATGTGATTTCGATGACGAGGGAGACGTGTCATGAGCTGCGGCCGCAGCTTCTGTATGAT
CTTGGATTGGTGAAGGCGCTGTCGAAGCTGGTGGCGCAGCAGCAGGAGCGGGTTCCGTTTCATATCCGTTTAAATACCGG
GAGATTTACGGCTTCCCTTGATCTGGATTCGCAGCTGAATTTGTACCGGATCATTCAAGAGTTTCTGTCTAATGCGGTCA
AGCACTCTCAGGCGACGGATGTGCTGATTATGCTCATCAGTATTCAAAACAAAATCGTTCTTCATTATGAGGACGATGGC
GTTGGGTTTGATCAAGAAAAAAATACTGAGCATTCCATGAGCATGGGGCTTTCTGGCATTAAGGAGAGAGTCAGGGCTTT
AGATGGGCGCCTTCGGATTGAAACAAGTGAAGGAAAGGGCTTTAAGGCTGATATTGAAATCGAATTGTAA

Domains

Predicted by InterproScan.

• Histidine kinase (HisKA_3) (568-638)

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (679-765)

3D structure

• AlphaFold DB: Q99027

References

• [1]: Xiaoyu Wang et al. (2010) Three non-aspartate amino acid mutations in the ComA Response regulator receiver motif severely decrease surfactin production, competence development and spore formation in Bacillus subtilis. Journal of Microbiology And Biotechnology 20(2):301-10. [PMID: 20208433]
• [2]: Natalia Comella et al. (2005) Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis. Molecular Microbiology 57(4):1159-74. [PMID: 16091051]
• [3]: F Piazza et al. (1999) Mutational analysis and membrane topology of ComP, a quorum-sensing histidine kinase of Bacillus subtilis controlling competence development. Journal of Bacteriology 181(15):4540-8. [PMID: 10419951]
• [4]: F Kunst et al. (1994) The DegS/DegU and ComP/ComA two-component systems are part of a network controlling degradative enzyme synthesis and competence in Bacillus subtilis. Research in Microbiology 145(5-6):393-402. [PMID: 7855425]
• [5]: R Magnuson et al. (1994) Biochemical and genetic characterization of a competence pheromone from B. subtilis. Cell 77(2):207-16. [PMID: 8168130]