Detailed information NTG0000126

Experimentally validated

Overview

• Name: rapC
• Type: Regulator
• Locus tag: BSU_03770
• Genome accession: NC_000964
• Coordinates: 428831..429979 (+)
• Length: 382 a.a.
• NCBI ID: NP_388259.1
• Uniprot ID: P94415
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: inhibit the DNA-binding function of ComA Competence regulation

Function

RapC directly interacts with the DNA-binding domain of ComA, preventing it from binding to target promoters and thereby repressing the transcription of competence genes.

Genomic Context

Location: 423831..434979

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSU_03730 (BSU03730)	yclH	424208..424888 (-)	681	NP_388255.1	putative ABC transporter (ATPase component)	-
BSU_03740 (BSU03740)	yclI	424904..426364 (-)	1461	NP_388256.2	putative transporter	-
BSU_03750 (BSU03750)	yclJ	426577..427260 (+)	684	NP_388257.1	two-component response regulator [YclK] (possibly involved in arabinogalactan metabolism)	-
BSU_03760 (BSU03760)	yclK	427247..428668 (+)	1422	NP_388258.1	two-component sensor histidine kinase [YclJ]	-
BSU_03770 (BSU03770)	rapC	428831..429979 (+)	1149	NP_388259.1	response regulator aspartate phosphatase	Regulator
BSU_03780 (BSU03780)	phrC	429963..430085 (+)	123	NP_388260.1	secreted regulator of the activity of phosphatase RapC and competence and sporulation stimulating factor (CSF)	Regulator
BSU_03788 (BSU03788)	yczM	430185..430274 (-)	90	YP_003097676.1	putative type I toxin	-
BSU_03789 (BSU03789)	yczN	430356..430469 (-)	114	YP_003097677.1	putative spore and germination protein	-
BSU_03790 (BSU03790)	thrD	430623..431987 (-)	1365	NP_388261.1	aspartate kinase III	-
BSU_03800 (BSU03800)	ceuB	432372..433322 (+)	951	NP_388262.1	petrobactin iron-siderophore ABC transporter (permease)	Machinery gene
BSU_03810 (BSU03810)	pbtO	433315..434262 (+)	948	NP_388263.1	petrobactin iron-siderophore ABC transporter (permease)	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
rapC	comA	negative effect
comA	comS	positive effect
comS	comK	positive effect
comK	comK	positive effect
comK	late competence genes	positive effect
codY	comK	negative effect
clpP	comK	negative effect
mecA	comK	negative effect
kre	comK	negative effect
abrB	comK	negative effect
spo0A	comK	positive effect
rok	comK	negative effect
degU	comK	positive effect
clpC	comK	negative effect
med	comK	positive effect
spo0A	comK	negative effect
comK	late competence genes	positive effect
clpP	degU	negative effect
abrB	rok	negative effect
spo0A	abrB	negative effect
spo0A	rok	negative effect
sinR	spo0A	negative effect
sinR	rok	negative effect
sinR	degU	negative effect
degQ	degU	positive effect
clpC	degU	negative effect
degS	degU	positive effect
rapF	comA	negative effect
comP	comA	positive effect
sinI	sinR	negative effect
phrC	rapC	negative effect
phrF	rapF	negative effect
comX	comP	positive effect
comQ	comX	positive effect

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 45634.68 Da
• Isoelectric Point: 4.9618

>NTDB_id=126 BSU_03770 NP_388259.1 428831..429979(+) (rapC) [Bacillus subtilis subsp. subtilis str. 168]
MKSGVIPSSAVGQKINEWYRYIRTFSVPDAEVLKAEIQQELKHMQHDSNLLLYYSLMEFRHQLMLDYLEPLEKLNIEDQP
SLSELSRNIDSNQADLKGLLDYYVNFFRGMYEFDKREFISAITYYKQAEKKLSFVADHIERAEFYFKIAEAYYYMKQTYF
SLINIKNAYEIYVEQETYNVRIIQCHFVFGVNLMDERNFEQAARHFKLALNMAQAEQKAQLVGRAYYNLGLCYYNQDLLD
PAIDYFEKAVSTFESSRIVNSLPQAYFLITLIYYKQGKHDKASEYHKRGYEYAKETDDADYAVKFEFLQSLYLDQPNEEG
IERCFQYLKNKNMYADIEDLALEVAKYYYEQKWFKLSASYFLQVEEARKQIQRSEGLYEIEI

Nucleotide

• Length: 1149 bp

>NTDB_id=126 BSU_03770 NP_388259.1 428831..429979(+) (rapC) [Bacillus subtilis subsp. subtilis str. 168]
ATGAAGAGTGGGGTAATTCCTTCTTCAGCGGTCGGTCAAAAAATTAACGAGTGGTACAGATATATCCGGACATTCAGCGT
GCCGGATGCCGAAGTGTTAAAAGCTGAAATCCAGCAGGAACTGAAACACATGCAGCATGATTCCAACTTGCTGCTTTATT
ATTCACTAATGGAATTCCGGCACCAGCTTATGCTGGATTATCTTGAGCCGTTAGAGAAATTAAATATCGAAGACCAGCCA
AGCCTGTCTGAATTATCAAGAAACATTGACAGCAACCAGGCAGATCTCAAAGGGCTGCTCGACTATTACGTGAATTTTTT
TCGCGGGATGTATGAATTTGATAAGCGGGAATTTATTTCTGCCATTACATACTATAAACAGGCGGAGAAAAAGCTCTCCT
TTGTCGCAGACCATATTGAACGGGCTGAATTCTATTTTAAAATCGCGGAAGCTTATTATTATATGAAGCAAACGTATTTT
TCATTGATTAATATAAAAAACGCCTATGAAATTTACGTGGAGCAGGAAACCTATAATGTGAGAATCATTCAGTGCCATTT
CGTCTTCGGGGTCAACCTGATGGATGAAAGAAATTTCGAACAAGCCGCACGCCATTTCAAATTGGCGCTCAACATGGCCC
AAGCAGAACAAAAAGCCCAGCTGGTTGGAAGAGCATACTACAATCTCGGGTTATGCTATTACAATCAAGACCTTCTAGAC
CCTGCCATTGATTACTTTGAAAAAGCGGTCTCCACATTTGAAAGCAGCAGGATCGTCAATTCTCTCCCGCAAGCCTATTT
TTTAATCACCCTGATTTATTATAAACAGGGAAAACATGATAAAGCTTCGGAATATCACAAGCGGGGCTATGAATATGCTA
AAGAAACAGACGATGCAGACTATGCCGTAAAATTCGAGTTTTTGCAATCCCTATATCTGGATCAGCCCAATGAAGAAGGA
ATCGAACGATGTTTCCAGTACTTAAAAAATAAAAATATGTACGCTGATATAGAGGATTTAGCCCTAGAAGTAGCAAAATA
TTACTATGAACAGAAATGGTTTAAACTGTCTGCTTCCTACTTTCTACAAGTTGAAGAGGCAAGAAAACAAATACAAAGGA
GTGAAGGTTTGTATGAAATTGAAATCTAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_12) (223-294)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-66)

3D structure

• AlphaFold DB: P94415

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572

References

• [1]: Ramses Gallegos-Monterrosa et al. (2021) Impact of Rap-Phr system abundance on adaptation of Bacillus subtilis. Communications Biology 4(1):468. [PMID: 33850233]
• [2]: Kassem Hamze et al. (2009) Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC. Microbiology (Reading, England) 155(Pt 2):398-412. [PMID: 19202088]
• [3]: Leighton Core et al. (2003) TPR-mediated interaction of RapC with ComA inhibits response regulator-DNA binding for competence development in Bacillus subtilis. Molecular Microbiology 49(6):1509-22. [PMID: 12950917]