Detailed information    

experimental Experimentally validated

Overview

Name   med   Type   Regulator
Locus tag   BSU_11300 Genome accession   NC_000964
Coordinates   1206629..1207582 (+) Length   317 a.a.
NCBI ID   NP_389012.2    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   regulation of regulators   
Competence regulation

Function

Med was found as a positive regulator for comK.


Genomic Context

Location: 1201629..1212582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_11250 (BSU11250) argF 1203461..1204420 (+) 960 NP_389007.1 ornithine carbamoyltransferase -
  BSU_11260 (BSU11260) yjzC 1204506..1204685 (+) 180 NP_389008.1 hypothetical protein -
  BSU_11270 (BSU11270) yjzD 1204731..1204916 (-) 186 NP_389009.1 forespore targeted protein -
  BSU_11280 (BSU11280) yjaU 1205165..1205899 (+) 735 NP_389010.1 hypothetical protein -
  BSU_11290 (BSU11290) yjaV 1205981..1206538 (+) 558 NP_389011.2 putative NAD(P) binding enzyme -
  BSU_11300 (BSU11300) med 1206629..1207582 (+) 954 NP_389012.2 positive regulator of comK Regulator
  BSU_11310 (BSU11310) comZ 1207597..1207788 (+) 192 NP_389013.1 putative late competence gene Regulator
  BSU_11320 (BSU11320) yjzB 1207818..1208057 (-) 240 NP_389014.1 spore coat protein -
  BSU_11330 (BSU11330) fabHA 1208222..1209160 (+) 939 NP_389015.1 beta-ketoacyl-acyl carrier protein synthase III 1 -
  BSU_11340 (BSU11340) fabF 1209183..1210424 (+) 1242 NP_389016.1 beta-ketoacyl-acyl carrier protein synthase II (involved in pimelate synthesis) -
  BSU_11350 (BSU11350) yjaZ 1210500..1211285 (+) 786 NP_389017.1 hypothetical protein -
  BSU_11360 (BSU11360) appD 1211477..1212463 (+) 987 NP_389018.1 oligopeptide ABC transporter (ATP-binding protein) -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  med comK positive effect
  comK comK positive effect
  comK late competence genes positive effect
  codY comK negative effect
  clpP comK negative effect
  mecA comK negative effect
  kre comK negative effect
  abrB comK negative effect
  comS comK positive effect
  spo0A comK positive effect
  rok comK negative effect
  degU comK positive effect
  clpC comK negative effect
  spo0A comK negative effect
  comK late competence genes positive effect
  clpP degU negative effect
  abrB rok negative effect
  spo0A abrB negative effect
  comA comS positive effect
  spo0A rok negative effect
  sinR spo0A negative effect
  sinR rok negative effect
  sinR degU negative effect
  degQ degU positive effect
  clpC degU negative effect
  degS degU positive effect
  rapC comA negative effect
  rapF comA negative effect
  comP comA positive effect
  sinI sinR negative effect
  phrC rapC negative effect
  phrF rapF negative effect
  comX comP positive effect
  comQ comX positive effect

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 35310.80 Da        Isoelectric Point: 4.5457

>NTDB_id=94 BSU_11300 NP_389012.2 1206629..1207582(+) (med) [Bacillus subtilis subsp. subtilis str. 168]
MITRLVMIFSVLLLLSGCGQTPFKGKIEKVGMLFPDTINDLVWGTKGYKGLLNIQSKYNVDVYYKEGVKTEEDIINAIED
FHKRGVNLLYGHGSEYAEVFNLVGEDYPDMEFVISNAKAKADNVTSVHFSGEAMGFFGGMTAAHMSKTNQVGVIASFTWQ
PEVDGFIKGAKYENPDIEVNTKYTDHWDDDTTAVKLYQKMKNEGADVVYPAGDGYNVPVIQQIKKDGLYAIGYVTDQSDL
GENTVLTSTVQNVDKAYEIIAEQFNKGTLEGGDHYYDLNTGVVEMGTFSPLVDQDFQQRIAKLIKTYNKTGELPKNE

Nucleotide


Download         Length: 954 bp        

>NTDB_id=94 BSU_11300 NP_389012.2 1206629..1207582(+) (med) [Bacillus subtilis subsp. subtilis str. 168]
TTGATCACAAGGCTTGTCATGATCTTTTCTGTCCTCCTTTTATTGAGTGGATGTGGACAAACTCCGTTCAAAGGAAAAAT
TGAGAAGGTCGGCATGCTCTTTCCTGATACGATTAATGACCTCGTATGGGGCACAAAAGGGTATAAAGGATTACTGAATA
TACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGAT
TTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTA
TCCAGATATGGAATTCGTCATTTCCAATGCGAAAGCGAAAGCTGATAATGTAACAAGTGTCCATTTTAGCGGGGAAGCAA
TGGGCTTTTTTGGAGGAATGACTGCTGCCCATATGTCGAAAACGAATCAGGTCGGTGTAATTGCTTCCTTTACGTGGCAG
CCTGAGGTTGACGGTTTTATCAAAGGGGCCAAGTATGAAAATCCGGATATAGAGGTAAATACGAAATATACGGATCATTG
GGATGATGATACAACAGCAGTAAAGCTTTATCAGAAAATGAAGAACGAAGGCGCGGATGTTGTGTATCCCGCCGGAGACG
GGTATAATGTTCCTGTTATTCAGCAAATCAAAAAAGACGGTCTTTATGCCATAGGCTACGTCACAGATCAATCGGATCTG
GGCGAGAATACCGTATTAACCAGCACGGTGCAAAATGTGGACAAGGCCTATGAAATCATTGCTGAACAATTTAATAAAGG
CACCCTTGAGGGCGGCGATCATTACTACGACCTGAACACGGGAGTCGTTGAGATGGGAACATTCAGCCCGCTCGTCGATC
AAGACTTTCAGCAAAGAATCGCCAAGCTGATCAAAACATACAACAAAACAGGCGAGCTGCCAAAAAACGAGTAA

Domains

Predicted by InterproScan.

ABC transporter substrate-binding protein PnrA-like (Bmp)

(27-317)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Mitsuo Ogura et al. (2002) Med, a cell-surface localized protein regulating a competence transcription factor gene, comK, in Bacillus subtilis. Bioscience, Biotechnology, And Biochemistry 66(4):892-6. [PMID: 12036071]
[2] M Ogura et al. (1997) A new Bacillus subtilis gene, med, encodes a positive regulator of comK. Journal of Bacteriology 179(20):6244-53. [PMID: 9335269]