Detailed information    

experimental Experimentally validated

Overview

Name   comFC   Type   Machinery gene
Locus tag   BSU_35450 Genome accession   NC_000964
Coordinates   3641125..3641847 (-) Length   240 a.a.
NCBI ID   NP_391425.2    Uniprot ID   A0A6M3ZH18
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

ComFC, together with its partner protein ComFA, facilicates the transport of single-stranded DNA across the cytoplasmic membrane.


Genomic Context

Location: 3636125..3646847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_35380 (BSU35380) fliW 3636264..3636695 (-) 432 NP_391418.1 assembly factor of the flagellum -
  BSU_35390 (BSU35390) yviE 3636716..3637291 (-) 576 NP_391419.1 hypothetical protein -
  BSU_35400 (BSU35400) flgL 3637338..3638234 (-) 897 NP_391420.1 flagellar hook-filament junction protein -
  BSU_35410 (BSU35410) flgK 3638245..3639768 (-) 1524 NP_391421.1 flagellar hook-filament junction -
  BSU_35420 (BSU35420) flgN 3639787..3640269 (-) 483 NP_391422.1 factor required for flagellar based motility -
  BSU_35430 (BSU35430) flgM 3640285..3640551 (-) 267 NP_391423.1 anti-sigma factor repressor of sigma(D)-dependent transcription -
  BSU_35440 (BSU35440) yvyF 3640632..3641051 (-) 420 NP_391424.1 putative transcriptional regulator of flagella formation -
  BSU_35450 (BSU35450) comFC 3641125..3641847 (-) 723 NP_391425.2 component of the DNA transport apparatus Machinery gene
  BSU_35460 (BSU35460) comFB 3641811..3642107 (-) 297 NP_391426.1 regulator of competence, pole located -
  BSU_35470 (BSU35470) comFA 3642167..3643558 (-) 1392 NP_391427.1 ATP-dependent helicase competence protein Machinery gene
  BSU_35480 (BSU35480) fakBA 3643664..3644509 (-) 846 NP_391428.1 fatty acid kinase fatty acid binding subunit A -
  BSU_35490 (BSU35490) degU 3644607..3645296 (-) 690 NP_391429.1 two-component response regulator Regulator
  BSU_35500 (BSU35500) degS 3645379..3646536 (-) 1158 NP_391430.1 two-component sensor histidine kinase [DegU] Regulator

Sequence

Protein


Download         Length: 240 a.a.        Molecular weight: 27477.75 Da        Isoelectric Point: 9.0021

>NTDB_id=109 BSU_35450 NP_391425.2 3641125..3641847(-) (comFC) [Bacillus subtilis subsp. subtilis str. 168]
MSNEAGGGIHKLICLLCDSQFSQDVTWRALFLLKPDEKVCYSCRSKLKKITGHICPLCGRPQSVHAVCRDCEVWRTRIRD
SLLLRQNRSVYTYNDMMKETLSRFKFRGDAEIINAFKSDFSSTFSKVYPDKHFVLVPIPLSKEREEERGFNQAHLLAECL
DRPSHHPLIRLNNEKQSKKKKTERLLSECIFDTKNNSAEGMNIILIDDLYTTGATLHFAARCLLEKGKAASVSSFTLIRS

Nucleotide


Download         Length: 723 bp        

>NTDB_id=109 BSU_35450 NP_391425.2 3641125..3641847(-) (comFC) [Bacillus subtilis subsp. subtilis str. 168]
ATGTCAAACGAAGCAGGAGGAGGCATTCATAAACTGATCTGTTTATTATGTGATTCGCAATTTTCTCAAGATGTTACATG
GAGAGCCCTTTTTTTACTAAAGCCTGATGAAAAGGTTTGTTATTCTTGCAGAAGCAAATTAAAGAAAATCACAGGACATA
TCTGCCCCCTGTGCGGCAGACCTCAATCTGTTCATGCCGTATGCAGGGATTGCGAGGTGTGGAGAACAAGGATCAGAGAC
AGTTTGCTATTGCGTCAAAACCGTTCAGTTTACACATATAATGATATGATGAAAGAAACGCTCTCCCGTTTTAAATTTAG
AGGAGATGCAGAAATCATAAATGCATTTAAAAGTGATTTTTCTTCAACCTTTTCTAAGGTTTACCCCGATAAGCATTTTG
TTCTTGTACCTATTCCGCTGAGCAAGGAACGGGAAGAAGAGCGCGGATTTAACCAGGCACATCTCTTGGCAGAATGTTTA
GACCGGCCTTCCCACCATCCCCTCATTCGCCTGAATAATGAGAAACAGTCAAAGAAAAAGAAAACAGAACGCCTGCTGTC
AGAGTGCATTTTTGATACAAAAAATAATTCAGCAGAAGGTATGAATATCATTTTAATAGACGATCTTTATACAACAGGCG
CCACCTTGCACTTCGCAGCCCGCTGCTTATTAGAAAAAGGAAAAGCCGCTTCAGTGTCATCTTTTACCTTGATCAGAAGC
TAA

Domains

Predicted by InterproScan.

Double zinc ribbon domain (DZR_2)

(14-71)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M3ZH18

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFC Lactococcus lactis subsp. cremoris KW2

37.063

100

0.396
  comFC/cflB Streptococcus mitis NCTC 12261

35.526

100

0.368
  comFC/cflB Streptococcus mitis SK321

35.088

100

0.364

Multiple sequence alignment    



References

[1] Micaela De Santis et al. (2021) ComEB protein is dispensable for the transformation but must be translated for the optimal synthesis of comEC. Molecular Microbiology 116(1):71-79. [PMID: 33527432]
[2] J A Londoño-Vallejo et al. (1993) comF, a Bacillus subtilis late competence locus, encodes a protein similar to ATP-dependent RNA/DNA helicases. Molecular Microbiology 9(1):119-31. [PMID: 8412657]