Detailed information    

experimental Experimentally validated

Overview

Name   comFC/cflB   Type   Machinery gene
Locus tag   SM12261_RS09235 Genome accession   NZ_CP028414
Coordinates   1835813..1836475 (-) Length   220 a.a.
NCBI ID   WP_000649953.1    Uniprot ID   A0AAX2L3H3
Organism   Streptococcus mitis NCTC 12261     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 1830813..1841475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS09210 (SM12261_1776) - 1830963..1831229 (-) 267 WP_001278169.1 Veg family protein -
  SM12261_RS09215 (SM12261_1777) dnaB 1831231..1832583 (-) 1353 WP_000852498.1 replicative DNA helicase -
  SM12261_RS09220 (SM12261_1778) rplI 1832627..1833079 (-) 453 WP_000864218.1 50S ribosomal protein L9 -
  SM12261_RS09225 (SM12261_1779) - 1833076..1835049 (-) 1974 WP_000715136.1 DHH family phosphoesterase -
  SM12261_RS09230 (SM12261_1780) hpf 1835185..1835733 (-) 549 WP_000599102.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SM12261_RS09235 (SM12261_1781) comFC/cflB 1835813..1836475 (-) 663 WP_000649953.1 ComF family protein Machinery gene
  SM12261_RS09240 (SM12261_1782) comFA/cflA 1836472..1837770 (-) 1299 WP_000867722.1 DEAD/DEAH box helicase Machinery gene
  SM12261_RS09245 (SM12261_1783) - 1837827..1838462 (+) 636 WP_000395748.1 YigZ family protein -
  SM12261_RS09250 (SM12261_1784) - 1838478..1838921 (+) 444 WP_000863906.1 PH domain-containing protein -
  SM12261_RS09255 (SM12261_1785) cysK 1839018..1839944 (+) 927 WP_000029880.1 cysteine synthase A -
  SM12261_RS09260 (SM12261_1786) tsf 1840066..1841106 (-) 1041 WP_000808076.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 220 a.a.        Molecular weight: 25218.29 Da        Isoelectric Point: 6.0413

>NTDB_id=508 SM12261_RS09235 WP_000649953.1 1835813..1836475(-) (comFC/cflB) [Streptococcus mitis NCTC 12261]
MKCLLCGQTMKAVLTFSSLLLLKNDASCLCLDCDSTFERIGEENCPNCMKTDLSMKCQDCQLWCKEGVEVSHRAIFIYNQ
AMKDFFSRYKFDGDFLLRKVFASVLSEELKKYKEYQFVVIPLSPERLLERGFNQVEGLVEAAGFAYLDLLEKREERASSS
KSRSERLETEFPFFIKSGVTIPKKILLIDDIYTTGTTINRVKKLLEEAGAEDVKTFSLVR

Nucleotide


Download         Length: 663 bp        

>NTDB_id=508 SM12261_RS09235 WP_000649953.1 1835813..1836475(-) (comFC/cflB) [Streptococcus mitis NCTC 12261]
ATGAAGTGTTTATTATGTGGGCAGACTATGAAGGCTGTTTTAACTTTCAGTAGTCTTTTACTTCTGAAGAATGATGCCTC
CTGTCTTTGTTTAGATTGTGATTCTACTTTTGAGAGGATTGGAGAAGAGAACTGCCCAAACTGTATGAAAACAGATTTGT
CAATGAAGTGTCAAGACTGTCAACTTTGGTGTAAAGAAGGAGTTGAGGTCAGTCATAGAGCGATTTTTATTTACAATCAA
GCTATGAAGGATTTTTTCAGTCGGTATAAGTTTGATGGAGACTTCCTTTTAAGAAAAGTTTTTGCTTCAGTTTTAAGTGA
GGAGTTAAAAAAGTACAAAGAGTATCAATTTGTTGTAATTCCCCTAAGTCCTGAAAGATTGCTCGAGAGGGGATTTAACC
AGGTTGAAGGTTTAGTTGAAGCAGCAGGGTTTGCGTATCTGGATTTATTAGAAAAGAGAGAAGAGCGAGCTAGTTCTTCT
AAAAGTCGCTCAGAGCGTTTGGAGACAGAATTTCCTTTCTTTATTAAAAGTGGAGTCACTATTCCTAAAAAAATCCTACT
TATAGATGATATTTACACTACTGGAACAACTATAAATCGTGTGAAGAAGCTGTTGGAAGAAGCGGGTGCTGAGGATGTAA
AAACATTTTCCCTTGTAAGATGA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(169-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFC/cflB Streptococcus mitis SK321

93.182

100

0.932
  comFC/cflB Streptococcus pneumoniae TIGR4

91.818

100

0.918
  comFC/cflB Streptococcus pneumoniae D39

91.364

100

0.914
  comFC/cflB Streptococcus pneumoniae Rx1

91.364

100

0.914
  comFC/cflB Streptococcus pneumoniae R6

91.364

100

0.914
  comFC Lactococcus lactis subsp. cremoris KW2

50.36

100

0.522
  comFC Bacillus subtilis subsp. subtilis str. 168

35.526

100

0.368

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]