Detailed information    

experimental Experimentally validated

Overview

Name   comFC/cflB   Type   Machinery gene
Locus tag   SP_RS11270 Genome accession   NC_003028
Coordinates   2127745..2128407 (-) Length   220 a.a.
NCBI ID   WP_000649971.1    Uniprot ID   A0A0B7MFB3
Organism   Streptococcus pneumoniae TIGR4     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

ComFC, together with its partner protein ComFA, facilicates the transport of single-stranded DNA across the cytoplasmic membrane.


Genomic Context

Location: 2122745..2133407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS11245 (SP_2202) - 2122895..2123161 (-) 267 WP_001278167.1 Veg family protein -
  SP_RS11250 (SP_2203) dnaB 2123163..2124515 (-) 1353 WP_000852486.1 replicative DNA helicase -
  SP_RS11255 (SP_2204) rplI 2124559..2125011 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  SP_RS11260 (SP_2205) - 2125008..2126981 (-) 1974 WP_000715115.1 DHH family phosphoesterase -
  SP_RS11265 (SP_2206) hpf 2127117..2127665 (-) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SP_RS11270 (SP_2207) comFC/cflB 2127745..2128407 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  SP_RS11275 (SP_2208) comFA/cflA 2128404..2129702 (-) 1299 WP_000867616.1 DEAD/DEAH box helicase Machinery gene
  SP_RS11280 (SP_2209) - 2129758..2130393 (+) 636 WP_000395742.1 YigZ family protein -
  SP_RS11285 (SP_2210) cysK 2130642..2131562 (+) 921 WP_000029866.1 cysteine synthase A -
  SP_RS11290 (SP_2211) - 2131637..2132175 (-) 539 Protein_2110 transposase -
  SP_RS11295 (SP_2212) - 2132148..2132435 (+) 288 Protein_2111 transposase -
  SP_RS11300 (SP_2213) tnpB 2132576..2132899 (-) 324 WP_000586642.1 IS66 family insertion sequence element accessory protein TnpB -

Sequence

Protein


Download         Length: 220 a.a.        Molecular weight: 25241.10 Da        Isoelectric Point: 7.3688

>NTDB_id=168 SP_RS11270 WP_000649971.1 2127745..2128407(-) (comFC/cflB) [Streptococcus pneumoniae TIGR4]
MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEENCPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQ
AMKDFFSRYKFDGDFLLRKVFASFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSLVR

Nucleotide


Download         Length: 663 bp        

>NTDB_id=168 SP_RS11270 WP_000649971.1 2127745..2128407(-) (comFC/cflB) [Streptococcus pneumoniae TIGR4]
ATGAAGTGCTTGTTATGTGGGCAGACTATGAAGACTGTTTTAACTTTTAGTAGTCTCTTACTTCTGAGGAATGATGACTC
TTGTCTTTGTTCAGACTGTGATTCTACTTTTGAAAGAATTGGGGAAGAGAACTGTCCAAATTGTATGAAAACAGAGTTGT
CAACAAAGTGTCAAGATTGTCAACTTTGGTGTAAAGAGGGAGTTGAAGTCAGTCATAGAGCGATTTTTACTTACAATCAA
GCTATGAAGGATTTTTTCAGTCGGTATAAGTTTGATGGAGACTTCCTGTTAAGAAAAGTTTTCGCTTCATTTTTAAGTGA
GGAGTTGAAAAAGTACAAAGAGTATCAATTTGTTGTAATTCCCCTAAGTCCTGATAGATATGCTAATAGAGGATTTAATC
AGGTTGAGGGCTTGGTAGAGGCAGCAGGCTTTGAGTATCTGGATTTATTAGAGAAAAGAGAAGAGAGAGCCAGTTCTTCT
AAAAATCGTTCAGAGCGCTTGGGGACAGAACTTCCTTTCTTTATTAAAAGTGGAGTCACTATTCCTAAAAAAATCCTACT
TATAGATGATATCTATACTACAGGAGCAACTATAAATCGTGTTAAGAAACTGTTGGAAGAAGCTGGTGCTAAGGATGTAA
AAACATTTTCCCTTGTAAGATGA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(153-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7MFB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFC/cflB Streptococcus pneumoniae Rx1

99.545

100

0.995
  comFC/cflB Streptococcus pneumoniae D39

99.545

100

0.995
  comFC/cflB Streptococcus pneumoniae R6

99.545

100

0.995
  comFC/cflB Streptococcus mitis NCTC 12261

91.818

100

0.918
  comFC/cflB Streptococcus mitis SK321

91.364

100

0.914
  comFC Lactococcus lactis subsp. cremoris KW2

48.921

100

0.507

Multiple sequence alignment    



References

[1] Amy Diallo et al. (2017) Bacterial transformation: ComFA is a DNA-dependent ATPase that forms complexes with ComFC and DprA. Molecular Microbiology 105(5):741-754. [PMID: 28618091]
[2] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]