Detailed information    

experimental Experimentally validated

Overview

Name   comFC/cflB   Type   Machinery gene
Locus tag   KZH43_RS10085 Genome accession   NZ_CP079923
Coordinates   1996868..1997530 (-) Length   220 a.a.
NCBI ID   WP_000649974.1    Uniprot ID   A0A0F7YBW3
Organism   Streptococcus pneumoniae Rx1     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

ComFC, together with its partner protein ComFA, facilicates the transport of single-stranded DNA across the cytoplasmic membrane.


Genomic Context

Location: 1991868..2002530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH43_RS10060 (KZH43_10055) - 1992018..1992284 (-) 267 WP_001278167.1 Veg family protein -
  KZH43_RS10065 (KZH43_10060) dnaB 1992286..1993638 (-) 1353 WP_000852486.1 replicative DNA helicase -
  KZH43_RS10070 (KZH43_10065) rplI 1993682..1994134 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  KZH43_RS10075 (KZH43_10070) - 1994131..1996104 (-) 1974 WP_000715109.1 DHH family phosphoesterase -
  KZH43_RS10080 (KZH43_10075) hpf 1996240..1996788 (-) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  KZH43_RS10085 (KZH43_10080) comFC/cflB 1996868..1997530 (-) 663 WP_000649974.1 ComF family protein Machinery gene
  KZH43_RS10090 (KZH43_10085) comFA/cflA 1997527..1998825 (-) 1299 WP_000867601.1 DEAD/DEAH box helicase Machinery gene
  KZH43_RS10095 (KZH43_10090) - 1998881..1999516 (+) 636 WP_000395739.1 YigZ family protein -
  KZH43_RS10100 (KZH43_10095) cysK 1999801..2000721 (+) 921 WP_000029860.1 cysteine synthase A -
  KZH43_RS10105 (KZH43_10100) - 2000960..2001310 (-) 351 Protein_1987 IS66 family transposase -
  KZH43_RS10110 (KZH43_10105) - 2001310..2001581 (+) 272 Protein_1988 transposase -
  KZH43_RS10115 (KZH43_10110) tnpB 2001772..2002059 (-) 288 WP_000973412.1 IS66 family insertion sequence element accessory protein TnpB -

Sequence

Protein


Download         Length: 220 a.a.        Molecular weight: 25169.03 Da        Isoelectric Point: 7.7116

>NTDB_id=278 KZH43_RS10085 WP_000649974.1 1996868..1997530(-) (comFC/cflB) [Streptococcus pneumoniae Rx1]
MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEENCPNCMKTELSTKCQDCQLWCKEGVGVSHRAIFTYNQ
AMKDFFSRYKFDGDFLLRKVFASFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSLVR

Nucleotide


Download         Length: 663 bp        

>NTDB_id=278 KZH43_RS10085 WP_000649974.1 1996868..1997530(-) (comFC/cflB) [Streptococcus pneumoniae Rx1]
ATGAAGTGCTTGTTATGTGGGCAGACTATGAAGACTGTTTTAACTTTTAGTAGTCTCTTACTTCTGAGGAATGATGACTC
TTGTCTTTGTTCAGACTGTGATTCTACTTTTGAAAGAATTGGGGAAGAGAACTGTCCAAATTGTATGAAAACAGAGTTGT
CAACAAAGTGTCAAGATTGTCAACTTTGGTGTAAAGAAGGAGTTGGAGTCAGTCATAGAGCGATTTTTACTTACAATCAA
GCTATGAAGGATTTTTTCAGTCGGTATAAGTTTGATGGAGACTTCCTGTTAAGAAAAGTTTTCGCTTCATTTTTAAGTGA
GGAGTTGAAAAAGTACAAAGAGTATCAATTTGTTGTAATTCCCCTAAGTCCTGATAGATATGCTAATAGAGGATTTAATC
AGGTTGAGGGCTTGGTAGAGGCAGCAGGCTTTGAGTATCTAGATTTATTAGAGAAAAGAGAAGAGAGAGCCAGTTCTTCT
AAAAATCGTTCAGAGCGCTTGGGGACAGAACTTCCTTTCTTTATTAAAAGTGGAGTCACTATTCCTAAAAAAATCCTACT
TATAGATGATATCTATACTACAGGAGCAACTATAAATCGTGTTAAGAAACTGTTGGAAGAAGCCGGTGCTAAGGATGTAA
AAACATTTTCCCTTGTAAGATGA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(153-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F7YBW3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFC/cflB Streptococcus pneumoniae D39

100

100

1
  comFC/cflB Streptococcus pneumoniae R6

100

100

1
  comFC/cflB Streptococcus pneumoniae TIGR4

99.545

100

0.995
  comFC/cflB Streptococcus mitis NCTC 12261

91.364

100

0.914
  comFC/cflB Streptococcus mitis SK321

90.909

100

0.909
  comFC Lactococcus lactis subsp. cremoris KW2

48.921

100

0.507

Multiple sequence alignment    



References

[1] Amy Diallo et al. (2017) Bacterial transformation: ComFA is a DNA-dependent ATPase that forms complexes with ComFC and DprA. Molecular Microbiology 105(5):741-754. [PMID: 28618091]
[2] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]