Detailed information NTG0000536

Experimentally validated

Overview

• Name: comFC/cflB
• Type: Machinery gene
• Locus tag: SMSK321_RS0102865
• Genome accession: NZ_AEDT01000032
• Coordinates: 205683..206345 (-)
• Length: 220 a.a.
• NCBI ID: WP_000649958.1
• Uniprot ID: -
• Organism: Streptococcus mitis SK321
• Function: ssDNA transport into the cell DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.

Genomic Context

Location: 200683..211345

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMSK321_RS10495 (SMSK321_1610)	-	200833..201099 (-)	267	WP_001278169.1	Veg family protein	-
SMSK321_RS10500 (SMSK321_1611)	dnaB	201101..202453 (-)	1353	WP_000852485.1	replicative DNA helicase	-
SMSK321_RS0102860	rplI	202497..202949 (-)	453	WP_000864214.1	50S ribosomal protein L9	-
SMSK321_RS10505 (SMSK321_1612)	-	202946..204919 (-)	1974	WP_000715142.1	DHH family phosphoesterase	-
SMSK321_RS10510 (SMSK321_1613)	hpf	205055..205603 (-)	549	WP_000599100.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
SMSK321_RS0102865	comFC/cflB	205683..206345 (-)	663	WP_000649958.1	ComF family protein	Machinery gene
SMSK321_RS10515 (SMSK321_1614)	comFA/cflA	206342..207640 (-)	1299	WP_000867726.1	DEAD/DEAH box helicase	Machinery gene
SMSK321_RS10520 (SMSK321_1615)	-	207697..208332 (+)	636	WP_000395766.1	YigZ family protein	-
SMSK321_RS12680	-	208426..208521 (+)	96	Protein_211	PH domain-containing protein	-
SMSK321_RS10525 (SMSK321_1616)	cysK	208618..209544 (+)	927	WP_000029882.1	cysteine synthase A	-
SMSK321_RS10530 (SMSK321_1617)	tsf	209666..210706 (-)	1041	WP_000808070.1	translation elongation factor Ts	-

Sequence

Protein

• Length: 220 a.a.
• Molecular weight: 25239.26 Da
• Isoelectric Point: 7.3707

>NTDB_id=536 SMSK321_RS0102865 WP_000649958.1 205683..206345(-) (comFC/cflB) [Streptococcus mitis SK321]
MKCLLCGQTMKAVLTFSSLLLLRNDVSCLCLDCDSTFERIGEENCPNCMKTALSTQCQDCQFWCKEGVEVSHRAIFIYNQ
AMKDFFSRYKFDGDFLLRKVFASVLSEELKKYKEYQFVVIPLSPERLLERGFNQVEGLVEAAGFSFQELLEKREERASSS
KNRSERLGTELPFFIKRGVTIPKKILLIDDIYTTGSTINRVKKLLEEAGAEDVKTFSLVR

Nucleotide

• Length: 663 bp

>NTDB_id=536 SMSK321_RS0102865 WP_000649958.1 205683..206345(-) (comFC/cflB) [Streptococcus mitis SK321]
ATGAAGTGCTTATTGTGTGGGCAGACTATGAAGGCTGTTTTAACTTTTAGTAGTCTCTTACTTCTGAGGAATGATGTCTC
CTGTCTTTGTTTAGATTGTGATTCTACTTTTGAGAGGATTGGAGAAGAGAACTGTCCAAACTGTATGAAAACAGCTTTGT
CAACGCAGTGTCAAGATTGTCAATTTTGGTGTAAAGAAGGAGTTGAGGTCAGTCATAGAGCGATTTTTATTTACAATCAA
GCTATGAAGGATTTTTTCAGTCGGTATAAATTTGACGGAGACTTCCTTTTAAGAAAAGTTTTTGCTTCAGTTTTAAGTGA
GGAGTTAAAAAAGTACAAAGAGTATCAATTTGTTGTAATTCCCCTAAGTCCTGAAAGATTGCTTGAGAGGGGATTTAACC
AGGTTGAAGGTTTAGTTGAAGCAGCAGGGTTCTCTTTTCAAGAGTTATTAGAGAAGAGAGAAGAGCGGGCCAGTTCTTCT
AAAAATCGTTCAGAACGCTTGGGGACAGAACTTCCTTTCTTTATTAAAAGGGGAGTCACTATTCCTAAAAAAATCCTACT
TATAGATGATATTTATACTACTGGATCTACTATAAATCGTGTGAAGAAACTGTTGGAGGAAGCTGGTGCTGAGGATGTCA
AAACATTTTCCCTTGTAAGATGA

Domains

Predicted by InterProScan.

• Phosphoribosyl transferase domain (Pribosyltran) (181-219)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFC/cflB	Streptococcus mitis NCTC 12261	93.182	100	0.932
comFC/cflB	Streptococcus pneumoniae TIGR4	91.364	100	0.914
comFC/cflB	Streptococcus pneumoniae D39	90.909	100	0.909
comFC/cflB	Streptococcus pneumoniae R6	90.909	100	0.909
comFC/cflB	Streptococcus pneumoniae Rx1	90.909	100	0.909
comFC	Lactococcus lactis subsp. cremoris KW2	48.201	100	0.5
comFC	Bacillus subtilis subsp. subtilis str. 168	35.088	100	0.364

References

• [1]: G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]