Detailed information    

experimental Experimentally validated

Overview

Name   amiA3   Type   Regulator
Locus tag   STER_RS06940 Genome accession   NC_008532
Coordinates   1321181..1323154 (-) Length   657 a.a.
NCBI ID   WP_011681419.1    Uniprot ID   -
Organism   Streptococcus thermophilus LMD-9     
Function   binding to XIP   
Competence regulation

Function

XIP peptides are recognized by the oligopeptide-binding protein AmiA3 and internalized by the oligopeptide Ami transporter (AmiCDEF).


Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 1319959..1328416 1321181..1323154 within 0


Gene organization within MGE regions


Location: 1319959..1328416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STER_RS06935 (STER_1410) - 1319959..1321136 (+) 1178 Protein_1298 ISL3 family transposase -
  STER_RS06940 (STER_1411) amiA3 1321181..1323154 (-) 1974 WP_011681419.1 peptide ABC transporter substrate-binding protein Regulator
  STER_RS06950 (STER_1413) - 1323459..1324606 (+) 1148 WP_095559415.1 IS3 family transposase -
  STER_RS10615 - 1324644..1324828 (-) 185 Protein_1301 IS3 family transposase -
  STER_RS11885 (STER_1414) - 1324822..1325232 (-) 411 Protein_1302 ATP-binding cassette domain-containing protein -
  STER_RS06965 (STER_1415) - 1325264..1325578 (-) 315 Protein_1303 TatD family hydrolase -
  STER_RS06970 (STER_1416) - 1325924..1327129 (+) 1206 WP_011681420.1 L-lactate MFS transporter -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  amiA3 comS positive effect
  comS comX positive effect
  comX late competence genes positive effect
  comX late competence genes positive effect
  comR comX positive effect
  clpP comX negative effect
  mecA comX negative effect
  clpC comX negative effect
  comR comS positive effect
  comS comS positive effect
  amiD comS positive effect
  pptA comS positive effect
  amiE comS positive effect
  eeP comS positive effect
  pptB comS positive effect
  amiF comS positive effect
  amiC comS positive effect

Sequence

Protein


Download         Length: 657 a.a.        Molecular weight: 72221.23 Da        Isoelectric Point: 5.1726

>NTDB_id=292 STER_RS06940 WP_011681419.1 1321181..1323154(-) (amiA3) [Streptococcus thermophilus LMD-9]
MKKSKWLALAGVALLGVGVLAACSSKSNTSGTTYGYVYSSDPETLDYITSNTGPTKSVVTNGVDGLMEADKYGNLVPSVA
EDWSVSKDGLTYTYKIRKGIKWYTSEGEEYANVTAKDFVTGLKHAADSKAGGIYLVQDSIAGLSDYLSGTNKDFSNVGVK
AIDDYTLQYTLKQPEPYWNSKTTYSLLFPVNEDFLKNKGKDFGKSTDPTSILYNGPFLLKSLTAKSSIELVKNEHYWDKK
NVHFDAIKFSYYDGSDQDALVRGFTDGAYNFARVFPTSSNYASVEKKYKDNIFYTAPGASTSAIGINIDRQSYKYTAKKT
DSEKSSTKKALLNKDFRQAINFAIDRKAYQSQINGKDGATLGVRNLFVPSDFVSAGDKTFGDLVTEKMSSYGDEWSGVNL
ADSQDGLYNAEKAKTEFAKAKDALQADGVQFPIHLDLPVNQSSKLNVAQVQSLKQTIEKSLGSENVVIDINQLSSDDLEN
ATLNAANAAAEDWDISNSVGWAPDYQDPSTYLDIFKTTSSENTKAFMGYDDPNNAAAAQVGLKDYDALLNSAASETSDLN
VRYDRYAQAQAWLEDSSLVIPLTVGNGAAPVISRLTPFTGASLQVGDKNSSNDYIKYVKPQEKVVTKKEYEQSREKWLKE
KKESNEKAQKDLEKHVK

Nucleotide


Download         Length: 1974 bp        

>NTDB_id=292 STER_RS06940 WP_011681419.1 1321181..1323154(-) (amiA3) [Streptococcus thermophilus LMD-9]
ATGAAAAAAAGCAAATGGTTGGCCCTCGCGGGTGTAGCACTTCTAGGTGTTGGTGTGTTAGCGGCTTGTTCATCTAAATC
AAACACTAGCGGAACTACTTATGGTTACGTTTACAGTTCGGATCCAGAAACTTTGGATTATATTACATCAAATACGGGAC
CTACAAAATCTGTTGTGACAAATGGTGTAGATGGACTTATGGAAGCGGACAAATATGGTAATCTTGTTCCATCAGTAGCT
GAAGACTGGTCTGTGTCTAAAGATGGTCTTACTTATACCTATAAAATTCGTAAAGGGATCAAATGGTATACTTCTGAAGG
TGAAGAGTACGCTAATGTTACTGCTAAGGACTTTGTAACGGGTCTTAAACATGCTGCGGATTCCAAAGCAGGTGGTATCT
ATTTGGTTCAAGATTCTATTGCTGGATTGTCTGACTATCTCTCTGGTACCAATAAAGATTTCTCGAATGTGGGTGTCAAG
GCAATTGATGACTATACTTTGCAGTACACGCTTAAGCAACCAGAACCATACTGGAACTCTAAGACAACTTATAGTTTGCT
TTTCCCAGTAAATGAAGACTTCCTCAAGAATAAGGGAAAAGATTTTGGTAAATCAACAGACCCAACCTCTATCCTTTATA
ATGGACCTTTCCTTCTCAAATCTTTGACAGCTAAATCTTCAATTGAGTTGGTTAAGAATGAACATTATTGGGACAAGAAA
AATGTACACTTTGATGCCATCAAATTCTCATATTATGATGGTTCGGATCAAGATGCTCTAGTACGTGGTTTCACTGATGG
TGCTTATAATTTTGCTCGTGTCTTTCCAACAAGTTCAAACTATGCTTCTGTAGAAAAAAAATATAAAGATAATATTTTTT
ACACAGCACCAGGTGCGTCTACATCAGCTATTGGTATTAATATTGACCGTCAAAGTTACAAGTATACAGCCAAAAAGACG
GATTCTGAAAAATCATCAACCAAGAAAGCCCTTCTAAACAAGGACTTCCGTCAAGCAATTAACTTTGCCATTGACCGTAA
GGCCTATCAATCACAAATTAACGGTAAAGATGGTGCAACTCTAGGTGTTCGTAACCTCTTTGTGCCTTCTGATTTTGTGA
GTGCAGGTGATAAGACCTTCGGAGATCTTGTAACAGAGAAAATGTCTTCATATGGGGATGAATGGTCAGGTGTGAATCTT
GCTGATAGTCAAGATGGCCTCTACAATGCTGAAAAAGCTAAGACTGAGTTTGCCAAAGCTAAGGATGCTCTCCAAGCAGA
TGGTGTTCAATTCCCAATTCATTTGGACCTTCCAGTTAACCAAAGCTCTAAACTTAATGTTGCCCAAGTCCAATCTTTGA
AACAAACCATTGAAAAATCATTGGGTAGCGAAAATGTTGTTATAGATATTAACCAATTGTCATCTGATGATTTGGAAAAT
GCAACACTTAATGCAGCCAACGCAGCAGCAGAAGACTGGGATATTTCAAATAGTGTCGGTTGGGCGCCAGATTACCAAGA
TCCATCAACCTACCTTGATATCTTTAAGACAACTTCAAGTGAAAACACTAAGGCTTTCATGGGTTATGATGATCCAAATA
ATGCGGCAGCAGCCCAAGTGGGACTCAAAGACTATGATGCCTTGCTGAATTCTGCAGCTTCAGAAACAAGTGACCTTAAT
GTTCGTTACGATCGTTATGCTCAAGCTCAAGCATGGTTGGAGGACAGTTCGCTTGTGATTCCATTGACTGTTGGTAATGG
TGCGGCACCAGTTATCTCACGCTTGACACCGTTTACCGGTGCTAGCCTCCAGGTGGGTGATAAAAACTCATCAAACGACT
ACATCAAATATGTGAAACCACAAGAAAAAGTAGTTACTAAGAAAGAATATGAACAAAGTCGTGAAAAATGGCTTAAAGAA
AAGAAAGAATCTAACGAAAAAGCTCAAAAAGATCTTGAGAAACACGTTAAATAA

Domains

Predicted by InterproScan.

Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5)

(75-520)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiA3 Streptococcus thermophilus LMG 18311

98.478

100

0.985
  amiA Streptococcus salivarius strain HSISS4

90.411

100

0.905

Multiple sequence alignment    



References

[1] Rozenn Gardan et al. (2013) Extracellular life cycle of ComS, the competence-stimulating peptide of Streptococcus thermophilus. Journal of Bacteriology 195(8):1845-55. [PMID: 23396911]
[2] Laetitia Fontaine et al. (2010) A novel pheromone quorum-sensing system controls the development of natural competence in Streptococcus thermophilus and Streptococcus salivarius. Journal of Bacteriology 192(5):1444-54. [PMID: 20023010]
[3] Rozenn Gardan et al. (2009) The oligopeptide transport system is essential for the development of natural competence in Streptococcus thermophilus strain LMD-9. Journal of Bacteriology 191(14):4647-55. [PMID: 19447907]