Detailed information    

experimental Experimentally validated

Overview

Name   pptA   Type   Regulator
Locus tag   STER_RS07730 Genome accession   NC_008532
Coordinates   1474027..1474758 (-) Length   243 a.a.
NCBI ID   WP_002951673.1    Uniprot ID   A0AAP2RM94
Organism   Streptococcus thermophilus LMD-9     
Function   export ComS   
Competence regulation

Function

PptAB controlled three regulation systems, two SHP/Rgg systems (SHP/Rgg1358 and SHP/Rgg1299), and the ComS/ComR system, while using transcriptional fusions and that PptAB helped to produce and export at least three different mature SHPs (SHP1358, SHP1299, and SHP279) peptides while using liquid chromatography-tandem mass spectrometry (LC-MS/MS).


Genomic Context

Location: 1469027..1479758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STER_RS07705 (STER_1567) - 1470076..1471257 (+) 1182 WP_002951656.1 NAD(P)/FAD-dependent oxidoreductase -
  STER_RS07715 (STER_1569) trmB 1471493..1472134 (-) 642 WP_002947526.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  STER_RS07720 (STER_1570) ccrZ 1472144..1472935 (-) 792 WP_002951667.1 cell cycle regulator CcrZ -
  STER_RS07725 (STER_1571) - 1472993..1474027 (-) 1035 WP_024703983.1 ABC transporter permease -
  STER_RS07730 (STER_1572) pptA 1474027..1474758 (-) 732 WP_002951673.1 ABC transporter ATP-binding protein Regulator
  STER_RS07735 (STER_1573) - 1474823..1475242 (+) 420 WP_011681530.1 HIT family protein -
  STER_RS07740 (STER_1574) - 1475239..1475538 (+) 300 WP_011681531.1 hypothetical protein -
  STER_RS07745 (STER_1575) - 1475590..1476267 (-) 678 WP_011681532.1 hypothetical protein -
  STER_RS07750 (STER_1576) - 1476337..1476972 (-) 636 WP_011681533.1 DUF4230 domain-containing protein -
  STER_RS12065 (STER_1577) - 1476991..1477116 (-) 126 WP_011681534.1 hypothetical protein -
  STER_RS07755 (STER_1578) - 1477534..1479633 (-) 2100 WP_011681535.1 ATP-dependent Clp protease ATP-binding subunit -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  pptA comS positive effect
  comS comX positive effect
  comX late competence genes positive effect
  comX late competence genes positive effect
  comR comX positive effect
  clpP comX negative effect
  mecA comX negative effect
  clpC comX negative effect
  comR comS positive effect
  comS comS positive effect
  amiD comS positive effect
  amiE comS positive effect
  eeP comS positive effect
  amiA3 comS positive effect
  pptB comS positive effect
  amiF comS positive effect
  amiC comS positive effect

Sequence

Protein


Download         Length: 243 a.a.        Molecular weight: 26830.05 Da        Isoelectric Point: 4.4606

>NTDB_id=481 STER_RS07730 WP_002951673.1 1474027..1474758(-) (pptA) [Streptococcus thermophilus LMD-9]
MLKLEKVTGGYANIPVLKDVTFEVADGELVGLIGLNGAGKSTTINEIIGLLTPYGGQIAIDGLTIQQDPASYRQKIGYIP
ETPSLYEELTLREHLETVLMAYGLDVTEGMARADKYLKLFRLDEKLDWFPTQFSKGMKQKVMIICAFIVNPSLFIVDEPF
LGLDPLAISDLIELLAEEKANGKSILMSTHVLDSAEKMCDRFVILHHGQILAQGTIDELRQTFGDSSASLNDIYMQLTKG
ELS

Nucleotide


Download         Length: 732 bp        

>NTDB_id=481 STER_RS07730 WP_002951673.1 1474027..1474758(-) (pptA) [Streptococcus thermophilus LMD-9]
ATGCTTAAATTAGAAAAGGTGACGGGTGGTTACGCTAATATTCCCGTCCTAAAAGATGTGACTTTTGAGGTGGCTGATGG
TGAATTGGTTGGTCTCATTGGTCTTAATGGTGCTGGAAAATCAACAACAATTAACGAGATTATTGGTCTCTTAACACCCT
ATGGAGGACAGATTGCCATTGATGGGTTAACTATTCAACAAGATCCAGCTAGCTATCGTCAGAAGATTGGCTATATTCCT
GAAACGCCAAGTCTTTATGAAGAATTGACGCTTCGTGAACATTTGGAAACGGTGTTGATGGCCTATGGTCTTGATGTGAC
TGAGGGTATGGCACGTGCGGACAAGTACCTCAAACTCTTTCGCTTAGATGAAAAGTTGGACTGGTTCCCAACTCAGTTTT
CAAAAGGGATGAAGCAGAAGGTCATGATTATCTGTGCCTTTATTGTGAATCCAAGTCTTTTCATTGTTGATGAGCCCTTT
TTGGGATTGGATCCCTTGGCGATTTCTGATTTGATTGAGCTTCTGGCTGAGGAAAAAGCCAATGGCAAGTCTATTCTCAT
GTCGACTCACGTTTTGGATTCAGCTGAGAAGATGTGTGATCGCTTTGTGATTTTGCACCATGGACAGATCTTGGCACAGG
GGACGATTGATGAGCTTCGTCAGACCTTTGGTGACAGTAGTGCTAGTCTTAACGATATCTATATGCAGTTGACTAAGGGA
GAATTGTCATGA

Domains

Predicted by InterproScan.

ABC transporter (ABC_tran)

(17-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pptA Streptococcus salivarius strain HSISS4

97.119

100

0.971

Multiple sequence alignment    



References

[1] Abarna Lingeswaran et al. (2020) Export of Rgg Quorum Sensing Peptides is Mediated by the PptAB ABC Transporter in Streptococcus Thermophilus Strain LMD-9. Genes 11(9):1096. [PMID: 32961685]