Detailed information    

experimental Experimentally validated

Overview

Name   eeP   Type   Regulator
Locus tag   STER_RS01205 Genome accession   NC_008532
Coordinates   209010..210272 (+) Length   420 a.a.
NCBI ID   WP_011680685.1    Uniprot ID   -
Organism   Streptococcus thermophilus LMD-9     
Function   processing of ComS   
Competence regulation

Function

At the cell surface, the protease Eep processes ComS, releasing XIP into the external milieu.


Genomic Context

Location: 204010..215272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STER_RS01180 (STER_0241) - 204591..205940 (+) 1350 WP_011680683.1 glucose-6-phosphate isomerase -
  STER_RS01185 (STER_0242) - 206128..206844 (+) 717 WP_002949309.1 YebC/PmpR family DNA-binding transcriptional regulator -
  STER_RS01190 (STER_0243) - 206970..207308 (+) 339 WP_011225395.1 preprotein translocase subunit YajC -
  STER_RS01195 (STER_0244) - 207437..208186 (+) 750 WP_002949314.1 isoprenyl transferase -
  STER_RS01200 (STER_0245) - 208199..208993 (+) 795 WP_011680684.1 phosphatidate cytidylyltransferase -
  STER_RS01205 (STER_0246) eeP 209010..210272 (+) 1263 WP_011680685.1 RIP metalloprotease RseP Regulator
  STER_RS01210 (STER_0247) - 210343..212205 (+) 1863 WP_011680686.1 proline--tRNA ligase -
  STER_RS01215 (STER_0249) - 212822..214762 (+) 1941 Protein_182 ABC transporter substrate binding protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  eeP comS positive effect
  comS comX positive effect
  comX late competence genes positive effect
  comX late competence genes positive effect
  comR comX positive effect
  clpP comX negative effect
  mecA comX negative effect
  clpC comX negative effect
  comR comS positive effect
  comS comS positive effect
  amiD comS positive effect
  pptA comS positive effect
  amiE comS positive effect
  amiA3 comS positive effect
  pptB comS positive effect
  amiF comS positive effect
  amiC comS positive effect

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45621.05 Da        Isoelectric Point: 6.5104

>NTDB_id=291 STER_RS01205 WP_011680685.1 209010..210272(+) (eeP) [Streptococcus thermophilus LMD-9]
MKAIITFLLIFCVIVVFHEFGHFFFAKRSGILVREFAIGMGPKIFAHTGKDGTVYTIRILPLGGYVRMAGWGEDTTEIKT
GSPASLTLGKDGKVRRINLSDRQVDQTALPMNVTAYDLEDKLTITGLVLGETKTYEVDHDATIVEEDGTELRIAPKDVQY
QNASIWGRLITNFAGPMNNFILGVLVFIILAFVQGGVQDTSTNLIQVANGGAAQVSGLKTGDAIVAINKDKVTDWDSLKE
ALRENTQKFSKGDSLSVTVKRSNGQEETISVKPQESQGSYFLGVSPVLKTGLKDKIFGGFQMAWEGATAILATLKGLITN
FSLNKLGGPVAMFQMSAQASESGLISILDLMGMLSINLGIFNLIPIPALDGGKIVMNIIEAIRRKPLNQEIESYITLAGV
AVMVVLMIAVTWNDIMRAFF

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=291 STER_RS01205 WP_011680685.1 209010..210272(+) (eeP) [Streptococcus thermophilus LMD-9]
ATGAAAGCTATTATTACTTTTTTACTGATTTTCTGTGTGATTGTCGTCTTCCATGAGTTTGGCCATTTCTTCTTTGCCAA
ACGATCAGGCATTCTGGTGCGTGAATTTGCCATTGGGATGGGTCCTAAAATTTTTGCACACACTGGTAAGGACGGTACCG
TTTATACTATTCGTATCCTTCCTTTGGGTGGTTATGTGCGTATGGCTGGTTGGGGTGAAGATACAACTGAGATTAAAACG
GGGAGTCCTGCTAGCCTAACGCTTGGAAAAGATGGCAAAGTGAGACGTATCAATCTTTCAGATCGTCAGGTTGACCAGAC
GGCTTTGCCTATGAACGTTACTGCCTATGATTTGGAAGATAAGTTAACTATTACAGGTCTTGTCCTAGGTGAGACCAAGA
CCTATGAGGTTGACCATGATGCCACTATCGTTGAGGAAGACGGTACGGAGCTTCGTATTGCTCCTAAGGATGTCCAATAT
CAAAATGCTAGCATTTGGGGACGTTTAATTACCAATTTTGCTGGTCCTATGAACAACTTTATTCTTGGGGTCTTGGTCTT
TATTATCCTTGCCTTTGTTCAAGGTGGTGTTCAAGATACGTCGACCAACCTTATCCAGGTGGCTAATGGTGGTGCTGCCC
AGGTGTCAGGTTTGAAAACTGGTGATGCCATCGTGGCCATTAACAAGGACAAGGTTACTGACTGGGACAGCTTGAAAGAA
GCATTGAGAGAGAATACCCAAAAATTCTCGAAAGGCGACAGCCTTTCTGTGACGGTTAAGAGAAGTAATGGTCAGGAAGA
AACTATTTCTGTTAAACCCCAAGAGAGCCAAGGGAGCTACTTTTTAGGTGTCTCTCCTGTCTTAAAGACAGGTCTTAAAG
ATAAGATTTTTGGTGGTTTCCAAATGGCTTGGGAAGGTGCGACGGCAATCCTCGCTACGCTCAAAGGGCTCATTACCAAC
TTTAGCTTGAACAAGCTTGGTGGACCGGTTGCTATGTTCCAGATGTCTGCACAAGCCTCAGAAAGTGGGTTGATTTCCAT
TCTTGACCTTATGGGAATGCTCTCTATCAACTTAGGAATTTTCAATCTTATTCCAATTCCAGCCCTTGACGGTGGTAAAA
TTGTTATGAATATTATTGAAGCTATTCGTCGTAAACCACTTAATCAAGAAATTGAAAGCTATATCACACTCGCAGGTGTT
GCGGTCATGGTTGTCCTTATGATTGCAGTAACTTGGAATGATATTATGCGTGCTTTCTTTTAA

Domains

Predicted by InterproScan.

PDZ domain (PDZ_2)

(204-272)

Peptidase family M50 (Peptidase_M50)

(6-406)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  eeP Streptococcus thermophilus LMG 18311

99.762

100

0.998

Multiple sequence alignment    



References

[1] Rozenn Gardan et al. (2013) Extracellular life cycle of ComS, the competence-stimulating peptide of Streptococcus thermophilus. Journal of Bacteriology 195(8):1845-55. [PMID: 23396911]