Detailed information    

experimental Experimentally validated

Overview

Name   covS   Type   Regulator
Locus tag   HSISS4_RS01435 Genome accession   NZ_CP013216
Coordinates   277100..278629 (+) Length   509 a.a.
NCBI ID   WP_002883784.1    Uniprot ID   A0A1V0GG48
Organism   Streptococcus salivarius strain HSISS4     
Function   repress comR expression   
Competence regulation

Function

CovRS, a well-described virulence regulatory system in pathogenic streptococci, directly controls the ComRS system to generate bimodality in the gut commensal Streptococcus salivarius and the closely related species Streptococcus thermophilus. CovRS is a direct repression system of comR expression.


Genomic Context

Location: 272100..283629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSISS4_RS01410 (HSISS4_00242) - 272566..273237 (-) 672 WP_021143560.1 potassium channel family protein -
  HSISS4_RS01415 (HSISS4_00243) - 273277..274641 (-) 1365 WP_081051370.1 TrkH family potassium uptake protein -
  HSISS4_RS01420 (HSISS4_00244) rsmG 274710..275423 (-) 714 WP_013990060.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  HSISS4_RS01425 (HSISS4_00245) - 275722..276258 (+) 537 WP_002883813.1 DUF177 domain-containing protein -
  HSISS4_RS01430 (HSISS4_00246) covR 276414..277103 (+) 690 WP_002883757.1 response regulator transcription factor Regulator
  HSISS4_RS01435 (HSISS4_00247) covS 277100..278629 (+) 1530 WP_002883784.1 HAMP domain-containing sensor histidine kinase Regulator
  HSISS4_RS01440 (HSISS4_00248) nrdR 278751..279230 (+) 480 WP_002883820.1 transcriptional regulator NrdR -
  HSISS4_RS01445 (HSISS4_00249) - 279227..280402 (+) 1176 WP_021143563.1 DnaD domain protein -
  HSISS4_RS01450 (HSISS4_00250) dnaI 280406..281308 (+) 903 WP_002883792.1 primosomal protein DnaI -
  HSISS4_RS01455 (HSISS4_00251) der 281413..282723 (+) 1311 WP_002886596.1 ribosome biogenesis GTPase Der -
  HSISS4_RS01460 (HSISS4_00252) - 282788..283417 (+) 630 WP_021143564.1 hypothetical protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  covS comR negative effect
  comR comX/sigX positive effect
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect
  stkP/pknB comX/sigX positive effect
  comS comX/sigX positive effect
  covR comR negative effect
  pptA comS positive effect
  amiD comS positive effect
  pptB comS positive effect
  amiE comS positive effect
  amiC comS positive effect
  amiF comS positive effect
  pepF comS negative effect
  amiA comS positive effect

Sequence

Protein


Download         Length: 509 a.a.        Molecular weight: 58071.44 Da        Isoelectric Point: 5.1853

>NTDB_id=472 HSISS4_RS01435 WP_002883784.1 277100..278629(+) (covS) [Streptococcus salivarius strain HSISS4]
MSKLQDKLFGKVTIRKKLTMTTAVVFFLVLSMFTLVVVFSANTLLLQRERQNVNTTISKVVTYIEKDWDSDEELSPEPLL
AALYSPKNIYASIINGVLSEKHSLDGQVAISNKLYSNQSVFVYDKKGTFIFTSEENTDSPPGMSEVNKLKSVSYKGKRGF
LLQVPIYGKDKKTIVGYAQIFHDLEFYYALKERLIFLLIFLEVGMTVLVIAATVVVLTSILRPMRQLHETMGVITDSPSD
LELRSKIESHDEIGDLAVNFNRMMDKIQENNQMQMRFLSDVSHELRTPIAVIKGHMDLLQRWGKNDPEILEESLEAASHE
ANRMTIMINDMLDSIRVKGSFENHRNDTCDLNSSIRTVIGNFRVLHEDYQFYLNDFESSERPAQIYSQHFEQAITILIDN
AVKYSPVNKEIQVTIKALEDEMLVQVQDNGEGISKEDIEHIFERFYRSDKARNRTTTQSGVGIGLSILYQIVEAYRCRID
VSSELGVGTRFDLYIPFADGETTTPQLEL

Nucleotide


Download         Length: 1530 bp        

>NTDB_id=472 HSISS4_RS01435 WP_002883784.1 277100..278629(+) (covS) [Streptococcus salivarius strain HSISS4]
ATGAGTAAATTACAAGATAAATTATTTGGTAAAGTAACCATTAGAAAAAAGTTGACCATGACAACTGCTGTGGTCTTCTT
TCTTGTATTGTCGATGTTCACTCTGGTTGTTGTTTTCTCAGCAAATACGCTACTTTTGCAACGTGAACGTCAAAATGTAA
ACACTACAATCTCAAAAGTTGTTACTTATATCGAAAAGGACTGGGACTCCGACGAGGAGTTGAGTCCAGAACCGCTTTTA
GCGGCCCTTTATTCGCCTAAAAATATCTATGCATCGATCATTAATGGTGTGCTTTCCGAAAAGCACTCTTTGGATGGTCA
GGTTGCGATTTCTAATAAATTATATTCTAACCAATCTGTTTTTGTTTATGACAAAAAGGGAACGTTTATCTTTACTTCTG
AGGAGAACACAGATTCGCCCCCAGGTATGTCCGAGGTAAACAAACTGAAGTCAGTTTCCTATAAAGGGAAGCGTGGTTTC
TTGCTGCAAGTTCCAATCTACGGTAAAGATAAAAAGACCATCGTTGGTTATGCCCAGATTTTCCATGATTTGGAATTCTA
CTATGCTCTTAAAGAGCGTTTGATTTTCTTATTGATTTTCCTAGAAGTGGGGATGACGGTCCTAGTTATCGCAGCGACTG
TAGTTGTCTTGACATCTATTCTTCGTCCGATGAGACAGTTGCACGAAACGATGGGAGTTATCACCGATTCGCCAAGTGAT
TTGGAACTTCGTTCTAAGATTGAAAGTCACGATGAAATTGGTGATTTGGCGGTTAACTTTAACCGTATGATGGATAAAAT
CCAAGAAAATAATCAGATGCAGATGCGTTTCTTGAGTGACGTCAGTCATGAATTGCGTACGCCGATTGCGGTTATCAAGG
GGCATATGGACCTCTTGCAACGTTGGGGTAAAAATGACCCTGAAATCCTTGAGGAGAGTCTTGAGGCTGCCAGTCACGAG
GCCAACCGTATGACGATTATGATTAATGACATGTTGGATTCTATCCGTGTCAAAGGGTCATTTGAAAATCACAGAAACGA
TACTTGTGATTTGAATTCTTCAATCCGTACGGTTATTGGTAACTTCCGTGTCTTGCATGAAGACTACCAATTCTATCTCA
ATGATTTCGAGAGCTCAGAGCGCCCTGCACAGATTTACAGCCAACACTTTGAACAGGCGATTACGATTTTGATTGATAAT
GCGGTCAAGTATTCTCCTGTTAACAAGGAAATCCAGGTTACAATTAAGGCTCTGGAAGATGAAATGTTGGTACAAGTGCA
AGATAATGGTGAAGGGATCTCTAAAGAAGATATTGAGCATATCTTTGAGCGCTTCTACCGTTCAGATAAGGCACGTAACC
GTACGACAACGCAATCTGGTGTTGGTATTGGTTTGTCAATCCTTTACCAAATTGTGGAAGCTTATCGTTGCCGTATCGAT
GTTAGTTCTGAACTTGGTGTTGGAACACGTTTCGACCTTTACATTCCATTTGCGGATGGAGAAACGACGACGCCTCAACT
TGAGCTTTAA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(388-497)

ArlS sensor domain (ArlS_N)

(43-191)

HAMP domain (HAMP)

(217-269)

His Kinase A (phospho-acceptor) domain (HisKA)

(274-333)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V0GG48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Adrien Knoops et al. (2022) The CovRS Environmental Sensor Directly Controls the ComRS Signaling System To Orchestrate Competence Bimodality in Salivarius Streptococci. MBio 13(1):e0312521. [PMID: 35089064]