Detailed information    

experimental Experimentally validated

Overview

Name   pepF   Type   Regulator
Locus tag   HSISS4_RS02090 Genome accession   NZ_CP013216
Coordinates   411028..412833 (+) Length   601 a.a.
NCBI ID   WP_002890133.1    Uniprot ID   -
Organism   Streptococcus salivarius strain HSISS4     
Function   degradation of XIP; competence shut-off   
Competence regulation

Function

By combining conditional mutants, transcriptional analyses, and biochemical characterization of pheromone degradation, we validated the reciprocal role of PepF and XIP in ComRS shut-off.


Genomic Context

Location: 406028..417833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSISS4_RS02080 (HSISS4_00367) - 406823..408760 (+) 1938 WP_059749081.1 GbpC/Spa domain-containing protein -
  HSISS4_RS02085 (HSISS4_00368) - 410058..411017 (+) 960 WP_021144611.1 competence protein CoiA -
  HSISS4_RS02090 (HSISS4_00369) pepF 411028..412833 (+) 1806 WP_002890133.1 oligoendopeptidase F Regulator
  HSISS4_RS02095 (HSISS4_00370) - 413050..414270 (+) 1221 WP_021144610.1 OFA family MFS transporter -
  HSISS4_RS02100 (HSISS4_00371) - 414342..415049 (+) 708 WP_014633986.1 O-methyltransferase -
  HSISS4_RS02105 (HSISS4_00372) - 415111..416253 (+) 1143 WP_021144608.1 peptidyl-prolyl cis-trans isomerase -
  HSISS4_RS10175 - 416322..416459 (+) 138 WP_156921847.1 hypothetical protein -
  HSISS4_RS02110 (HSISS4_00373) - 416592..417077 (+) 486 Protein_374 LURP-one-related/scramblase family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  pepF comS negative effect
  comS comX/sigX positive effect
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect
  stkP/pknB comX/sigX positive effect
  comR comX/sigX positive effect
  covS comR negative effect
  covR comR negative effect
  pptA comS positive effect
  amiD comS positive effect
  pptB comS positive effect
  amiE comS positive effect
  amiC comS positive effect
  amiF comS positive effect
  amiA comS positive effect

Sequence

Protein


Download         Length: 601 a.a.        Molecular weight: 68998.04 Da        Isoelectric Point: 4.5805

>NTDB_id=480 HSISS4_RS02090 WP_002890133.1 411028..412833(+) (pepF) [Streptococcus salivarius strain HSISS4]
MSDNRTHLEEKYTWDLTTIFATDADWETEYESTVQDLKKASAYAGHLLDSAKNLLGATELYMSLMRRLEKIYVYASMKND
QDTTVGLYQEYQAKASNLYSQLSEAFAYFEPEFMALDDKKLAEFKEQEPGLGLYDHYFERLLANKDHVLSQEAEELLAAA
GDIFNGPTDTFNVLDNADILFPWVSDGQGDVVELTHGNFITLMESKDRDIRKGAYEAMYGTYEQFQHTYAQTLQGVVKVH
NYQAKVRHYNSARHAALAANFIPESVYDSLLESVNKHLPLLHRYLDLRKKVLGLDELKMYDVYTPLSETETALTYEESLK
KAEEVLAIFGEEYSKGVHAAFTERWIDVHPNKGKRSGAYSGGAYDTNAFMLLNWQDTLDNLFTLVHETGHSLHSTFTRQT
QPYVYGDYPIFLAEIASTTNENILTETLLKEVKDDKTRFAILNHYLDGFKGTVFRQTQFAEFEHAIHEADASGQILTADF
MNKLYADLNEKYYNLKAEDNYEIQFEWERIPHFYMNYYVYQYATGFAAASYLAEKIVHGTEEDKEAYLTYLKAGSSDYPL
EVIKKAGVDMTNTDYLDAAFKVFEDRLVELEALVEKGVHLS

Nucleotide


Download         Length: 1806 bp        

>NTDB_id=480 HSISS4_RS02090 WP_002890133.1 411028..412833(+) (pepF) [Streptococcus salivarius strain HSISS4]
ATGTCAGACAATCGTACTCATTTAGAAGAAAAATACACATGGGATTTGACGACCATTTTTGCGACAGATGCAGATTGGGA
GACCGAATATGAAAGCACTGTTCAGGATTTGAAGAAGGCTAGTGCTTATGCAGGTCATCTCTTGGATTCTGCTAAGAACT
TGCTTGGGGCAACAGAACTTTATATGAGCCTTATGCGTCGCTTGGAGAAAATCTATGTCTATGCGTCAATGAAAAATGAC
CAAGACACAACAGTAGGTCTGTATCAAGAGTACCAAGCAAAGGCCTCAAACCTTTATTCACAGTTGAGTGAAGCCTTTGC
TTACTTTGAGCCTGAATTTATGGCCTTGGATGATAAGAAATTAGCTGAATTTAAAGAACAAGAGCCAGGTCTTGGACTTT
ATGACCACTATTTCGAACGTCTTTTGGCCAACAAGGATCACGTTCTTTCTCAAGAAGCTGAAGAACTCTTGGCAGCAGCC
GGTGATATTTTCAACGGTCCAACGGATACCTTTAATGTCTTGGATAATGCTGATATCCTCTTCCCATGGGTATCAGATGG
CCAAGGGGATGTGGTTGAGTTGACACATGGTAACTTTATCACCCTCATGGAATCTAAGGACCGTGACATTCGTAAGGGTG
CTTATGAAGCGATGTACGGTACTTACGAGCAGTTCCAACATACCTATGCACAAACACTTCAAGGCGTTGTTAAGGTTCAC
AATTACCAAGCCAAAGTTCGTCACTATAATTCGGCACGTCATGCAGCACTTGCGGCTAATTTTATTCCAGAGAGTGTTTA
TGACTCACTCTTGGAATCAGTGAATAAGCATTTGCCACTTTTGCACCGTTACCTTGATTTGCGTAAGAAGGTCTTGGGGC
TTGACGAGCTTAAGATGTATGACGTCTACACACCACTTTCTGAGACTGAAACTGCTCTTACTTATGAAGAGTCCCTCAAG
AAAGCAGAAGAAGTCTTGGCAATCTTTGGTGAAGAGTATAGTAAAGGGGTTCATGCAGCCTTTACGGAACGTTGGATTGA
CGTTCACCCTAACAAAGGAAAACGCTCAGGTGCCTACTCAGGCGGTGCCTATGATACCAATGCTTTCATGCTTTTGAACT
GGCAAGACACTCTGGACAATCTCTTTACCTTGGTTCACGAGACTGGGCACAGCTTGCATTCAACGTTCACACGTCAGACA
CAACCATATGTTTATGGAGATTACCCAATCTTCTTGGCGGAAATTGCGTCTACAACTAATGAAAATATCTTGACAGAAAC
ACTGCTTAAAGAGGTTAAAGATGACAAGACACGTTTTGCTATTCTTAACCACTATTTGGATGGCTTCAAGGGAACTGTCT
TCCGTCAAACCCAATTTGCTGAGTTTGAGCATGCTATCCATGAAGCAGATGCCTCTGGTCAAATCTTGACAGCCGACTTT
ATGAATAAACTTTACGCAGACCTCAATGAGAAATACTATAACCTTAAAGCTGAGGATAACTACGAAATTCAGTTTGAGTG
GGAACGTATTCCGCATTTCTACATGAATTACTATGTCTACCAATACGCTACAGGGTTTGCAGCAGCAAGCTACTTGGCTG
AAAAAATTGTTCACGGTACTGAGGAGGATAAAGAAGCTTACCTTACTTACCTTAAGGCGGGAAGCTCAGATTATCCTTTG
GAAGTTATCAAGAAAGCTGGTGTTGATATGACCAATACCGACTACTTGGATGCTGCCTTCAAAGTTTTTGAAGACCGTCT
CGTTGAATTGGAAGCCTTGGTTGAAAAAGGTGTTCACCTTTCTTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(112-181)

Peptidase family M3 (Peptidase_M3)

(202-581)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Adrien Knoops et al. (2022) Competence shut-off by intracellular pheromone degradation in salivarius streptococci. PLoS Genetics 18(5):e1010198. [PMID: 35613247]