Detailed information    

experimental Experimentally validated

Overview

Name   mecA   Type   Regulator
Locus tag   KZH43_RS05975 Genome accession   NZ_CP079923
Coordinates   1210450..1211187 (-) Length   245 a.a.
NCBI ID   WP_000782664.1    Uniprot ID   -
Organism   Streptococcus pneumoniae Rx1     
Function   degradation of ComW   
Competence regulation

Function

ComW is a target of the Clp machinery and was shown to be degraded by ClpCP. This degradation is likely to require the adaptor protein MecA, an obligate partner of ClpC, as MecA inactivation leads to ComW stabilisation.


Genomic Context

Location: 1205450..1216187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH43_RS05955 (KZH43_05955) msrB 1206908..1207846 (-) 939 WP_000818207.1 peptide-methionine (R)-S-oxide reductase MsrB -
  KZH43_RS10595 - 1207875..1208066 (-) 192 WP_000693310.1 hypothetical protein -
  KZH43_RS05965 (KZH43_05965) thrB 1208142..1209011 (-) 870 WP_000692438.1 homoserine kinase -
  KZH43_RS05970 (KZH43_05970) - 1209013..1210299 (-) 1287 WP_000216382.1 homoserine dehydrogenase -
  KZH43_RS05975 (KZH43_05975) mecA 1210450..1211187 (-) 738 WP_000782664.1 adaptor protein MecA Regulator
  KZH43_RS05980 (KZH43_05980) - 1211329..1212363 (-) 1035 WP_000461498.1 hypothetical protein -
  KZH43_RS05985 (KZH43_05985) - 1212392..1213825 (-) 1434 WP_000565088.1 O-antigen polysaccharide polymerase Wzy family protein -
  KZH43_RS05990 (KZH43_05990) - 1213841..1214827 (-) 987 WP_220041224.1 glycosyltransferase family 2 protein -
  KZH43_RS05995 (KZH43_05995) - 1214829..1215932 (-) 1104 WP_000225896.1 glycosyltransferase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  mecA comW negative effect
  comW comX/comX1 positive effect
  comX/comX1 late competence genes positive effect
  comX/comX1 late competence genes positive effect
  clpE comX/comX1 negative effect
  comE comX/comX1 positive effect
  clpP comX/comX1 negative effect
  comX/comX2 late competence genes positive effect
  comX/comX2 late competence genes positive effect
  comW comX/comX2 positive effect
  clpE comX/comX2 negative effect
  comE comX/comX2 positive effect
  clpP comX/comX2 negative effect
  clpC comW negative effect
  clpP comW negative effect
  comE comW positive effect
  comE comA positive effect
  comE comB positive effect
  comE comE positive effect
  comE comC/comC1 positive effect
  comE comD/comD1 positive effect
  comE comM positive effect
  comD/comD1 comE positive effect
  stkP comE positive effect
  comA comC/comC1 positive effect
  comB comC/comC1 positive effect
  comC/comC1 comD/comD1 positive effect
  ciaR comC/comC1 negative effect
  ciaH comC/comC1 negative effect
  htrA comC/comC1 negative effect
  comM cbpD negative effect
  ciaR htrA positive effect
  ciaH htrA positive effect
  htrA comEA/celA/cilE negative effect
  htrA comEC/celB negative effect
  cbpD lytA positive effect
  cbpD lytC positive effect

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 28363.15 Da        Isoelectric Point: 4.0199

>NTDB_id=286 KZH43_RS05975 WP_000782664.1 1210450..1211187(-) (mecA) [Streptococcus pneumoniae Rx1]
MKMKQISDTTLKITISLEDLMDRGMEIADFLVPQEKTEEFFYAILDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKI
DQNLDFEDLSDLPDMEELAQMSPDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDSDISRSVLQEYGQVLMSHDAVLN
LQKIG

Nucleotide


Download         Length: 738 bp        

>NTDB_id=286 KZH43_RS05975 WP_000782664.1 1210450..1211187(-) (mecA) [Streptococcus pneumoniae Rx1]
ATGAAAATGAAACAAATTAGTGATACAACTTTAAAAATCACGATATCTTTAGAGGATTTGATGGATCGTGGAATGGAGAT
TGCTGACTTTCTCGTTCCTCAAGAAAAAACAGAAGAGTTCTTTTATGCTATCTTGGATGAGCTAGAGATGCCTGATAGCT
TTCTGGATACAGGCATGTTGAGCTTCCGTGTGACTCCAAAACCTGATAAGGTAGATGTCTTTGTGACCAAGTCAAAGATT
GACCAAAATCTAGATTTTGAAGACTTATCGGATTTGCCAGATATGGAAGAATTGGCTCAAATGTCTCCAGATGAATTTAT
CAAAACCCTGGAAAAAAGCATCGCAGACAAAACCAAGGATGATATCGAAGCCATTCAATCTCTTGAGCAAGTTGAAGCCA
AGGAAGAAGAGCAAGAGCAGGCTGAACAAGAAGCTGAGAGTAAGAAAGAACCTTACATCTACTACATCCTTTCTTTTGCT
AAGTTGGCTGACTTGGTAGCTTTTGCCAAGACAGTGACTTTTGAAATGGAAACTTCTGAACTCTACAAAATGAACGAGCG
CTATTATTTGACCATTTTAGTGGATATTGAAAATCATCCAAGCCCATATCCAGCTTGGCTGTTGGCCCGTATGCGCGAGT
TTGCAGACGATAGTGATATCAGTCGCTCAGTCTTACAAGAGTATGGTCAAGTCTTGATGAGTCACGATGCAGTGCTCAAT
CTGCAAAAAATCGGCTAA

Domains

Predicted by InterproScan.

Negative regulator of genetic competence (MecA) (MecA)

(1-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mecA Streptococcus pneumoniae D39

100

100

1
  mecA Streptococcus pneumoniae R6

100

100

1
  mecA Streptococcus pneumoniae TIGR4

99.592

100

0.996
  mecA Streptococcus mutans UA159

48.571

100

0.496
  mecA Streptococcus thermophilus LMD-9

48.193

100

0.49
  mecA Streptococcus thermophilus LMG 18311

47.791

100

0.486

Multiple sequence alignment    



References

[1] Laetitia Fontaine et al. (2015) Regulation of competence for natural transformation in streptococci. Infection, Genetics And Evolution : Journal of Molecular Epidemiology And Evolutionary Genetics in Infectious Diseases 33:343-60. [PMID: 25236918]