Detailed information    

experimental Experimentally validated

Overview

Name   comM   Type   Regulator
Locus tag   KZH43_RS08690 Genome accession   NZ_CP079923
Coordinates   1721168..1721789 (-) Length   206 a.a.
NCBI ID   Protein_1730    Uniprot ID   -
Organism   Streptococcus pneumoniae Rx1     
Function   fratricide immunity   
Competence regulation

Function

The comM gene encodes a protein that provides cellular immunity towards lytic enzymes, such as the murein hydrolase CbpD, which is involved in fratricide during competence. ComM protects competent cells from the lytic enzymes produced during the competence state, allowing them to survive and undergo natural transformation.


Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1710436..1732857 1721168..1721789 within 0


Gene organization within MGE regions


Location: 1710436..1732857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH43_RS08625 (KZH43_08620) - 1710436..1710633 (-) 198 WP_000890144.1 hypothetical protein -
  KZH43_RS08630 (KZH43_08625) - 1710637..1711431 (-) 795 WP_001101210.1 hypothetical protein -
  KZH43_RS08635 (KZH43_08630) - 1711839..1712264 (-) 426 WP_000204065.1 hypothetical protein -
  KZH43_RS08640 (KZH43_08635) - 1712393..1712752 (-) 360 WP_000749822.1 ImmA/IrrE family metallo-endopeptidase -
  KZH43_RS08645 (KZH43_08640) - 1712798..1713004 (-) 207 WP_000366091.1 hypothetical protein -
  KZH43_RS08650 (KZH43_08645) - 1712991..1713164 (-) 174 WP_000289349.1 helix-turn-helix domain-containing protein -
  KZH43_RS08655 (KZH43_08650) lytA 1713536..1714492 (-) 957 WP_000405235.1 N-acetylmuramoyl-L-alanine amidase LytA Auxiliary factor
  KZH43_RS08660 (KZH43_08655) - 1714868..1716238 (-) 1371 WP_001036271.1 MATE family efflux transporter -
  KZH43_RS08665 (KZH43_08660) recA 1716545..1717711 (-) 1167 WP_001085462.1 recombinase RecA Machinery gene
  KZH43_RS08670 (KZH43_08665) cinA 1717766..1719022 (-) 1257 WP_000642700.1 competence/damage-inducible protein A Machinery gene
  KZH43_RS08675 (KZH43_08670) - 1719107..1720123 (-) 1017 WP_000239282.1 LCP family protein -
  KZH43_RS08680 (KZH43_08675) - 1720131..1720649 (-) 519 WP_000455537.1 GNAT family N-acetyltransferase -
  KZH43_RS08685 (KZH43_08680) tsaE 1720639..1721082 (-) 444 WP_000288232.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
  KZH43_RS08690 (KZH43_08685) comM 1721168..1721789 (-) 622 Protein_1730 hypothetical protein Regulator
  KZH43_RS08695 (KZH43_08690) - 1722172..1723035 (-) 864 WP_001093641.1 helix-turn-helix domain-containing protein -
  KZH43_RS10705 - 1723227..1723397 (+) 171 WP_001227862.1 PhrA family quorum-sensing system peptide -
  KZH43_RS08700 (KZH43_08695) pneA1 1723500..1723724 (+) 225 WP_000181087.1 two-peptide bacteriocin subunit PneA1 -
  KZH43_RS08705 (KZH43_08700) pneA2 1723731..1723919 (+) 189 WP_000786759.1 two-peptide bacteriocin subunit PneA2 -
  KZH43_RS08710 (KZH43_08705) - 1724035..1727025 (+) 2991 WP_000875575.1 type 2 lanthipeptide synthetase LanM family protein -
  KZH43_RS08715 (KZH43_08710) - 1727375..1728079 (+) 705 WP_001033170.1 flavodoxin family protein -
  KZH43_RS08720 (KZH43_08715) - 1728060..1728989 (+) 930 WP_000912954.1 M50 family metallopeptidase -
  KZH43_RS08725 (KZH43_08720) - 1728986..1731094 (+) 2109 WP_000701298.1 peptidase domain-containing ABC transporter -
  KZH43_RS08730 (KZH43_08725) - 1731118..1732857 (+) 1740 WP_000699969.1 S8 family serine peptidase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comM cbpD negative effect
  cbpD lytA positive effect
  cbpD lytC positive effect
  comE comM positive effect
  comE comA positive effect
  comA comC/comC1 positive effect
  comE comB positive effect
  comB comC/comC1 positive effect
  comE comE positive effect
  comE comC/comC1 positive effect
  comE comX/comX1 positive effect
  comE comX/comX2 positive effect
  comE comD/comD1 positive effect
  comE comW positive effect
  comD/comD1 comE positive effect
  stkP comE positive effect
  comC/comC1 comD/comD1 positive effect
  ciaR comC/comC1 negative effect
  ciaH comC/comC1 negative effect
  htrA comC/comC1 negative effect
  comX/comX1 late competence genes positive effect
  comW comX/comX1 positive effect
  clpE comX/comX1 negative effect
  clpP comX/comX1 negative effect
  comX/comX2 late competence genes positive effect
  comW comX/comX2 positive effect
  clpE comX/comX2 negative effect
  clpP comX/comX2 negative effect
  clpC comW negative effect
  clpP comW negative effect
  mecA comW negative effect
  ciaR htrA positive effect
  ciaH htrA positive effect
  htrA comEA/celA/cilE negative effect
  htrA comEC/celB negative effect
  comX/comX1 late competence genes positive effect
  comX/comX2 late competence genes positive effect

Sequence

Protein


Download         Length: 206 a.a.        Molecular weight: Da        Isoelectric Point:

>NTDB_id=253 KZH43_RS08690 Protein_1730 1721168..1721789(-) (comM) [Streptococcus pneumoniae Rx1]
None

Nucleotide


Download         Length: 622 bp        

>NTDB_id=253 KZH43_RS08690 Protein_1730 1721168..1721789(-) (comM) [Streptococcus pneumoniae Rx1]
ATGAAATCAATGAGAATCTTATTTTTGTTAGCTTTAATTCAAATCAGTTTGAGTAGCTGTTTCCTATGGAAGGAATGCAT
CTTGTCCTTTAAACAAAGTACAGCTTTTTTCATCGGAAGCATGGTTTTCGTTTCAGGAATCTGTGCTGGAGTAAATTATC
TTTATACCCGTAAGCAAGAAGTCCATAGTGTCCTAGCCAGTAAGAAGTCGGTGAAGCTTTTTTACAGTATGTTACTCTTA
ATTAATTTGTTAGGAGCTGTTCTTGTTTTGTCAGATAACTTGTTCATCAAAAATACGCTGCAGCAAGAATTAGTTGACTT
TTTATTGCCATCCTTCTTTTTCCTATTTGGGCTAGATTTGCTGATTTTTTTTACCCTTGAAAAAATACGTGCGCGATTTT
CTTGCTATGCTGGACAGAAAAAAGACAGTGTTGGTGACTATTTTAGCAACACTTCTTTTCTTAAGAAATCCAATGACCAT
TGTCTCACTTCTGATTTATATTGGACTGGGCTTGTTTTTTGCAGCCTATCTTGTCCCAAATTCGGTTAAGAAGGAAGTTT
CCTTTTATGGTCATATTTTCCGAGATCTTGTATTGGTCATTGTTACGCTCATTTTCTTTTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Daniel Straume et al. (2017) Overexpression of the fratricide immunity protein ComM leads to growth inhibition and morphological abnormalities in Streptococcus pneumoniae. Microbiology (Reading, England) 163(1):9-21. [PMID: 27902435]
[2] Leiv Sigve Håvarstein et al. (2006) New insights into the pneumococcal fratricide: relationship to clumping and identification of a novel immunity factor. Molecular Microbiology 59(4):1297-307. [PMID: 16430701]
[3] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]