Detailed information    

experimental Experimentally validated

Overview

Name   ciaH   Type   Regulator
Locus tag   KZH43_RS03480 Genome accession   NZ_CP079923
Coordinates   703764..705098 (+) Length   444 a.a.
NCBI ID   WP_000491790.1    Uniprot ID   Q7B3G0
Organism   Streptococcus pneumoniae Rx1     
Function   repress competence development; post-transcriptional repression of CSP production   
Competence regulation

Function

CiaRH negatively regulates competence development by controlling the expression of csRNAs (cia-dependent small RNAs) that target comC. These csRNAs post-transcriptionally regulate comC, thereby influencing the initiation of the competence cascade. A hyperactive CiaRH system might suppress competence development by increasing the level of HtrA at the outer surface of the cytoplasmic membrane.


Genomic Context

Location: 698764..710098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH43_RS03460 (KZH43_03465) - 699038..699907 (+) 870 WP_000795797.1 putative PEP-binding protein -
  KZH43_RS03465 (KZH43_03470) - 699882..700322 (+) 441 WP_001856133.1 ASCH domain-containing protein -
  KZH43_RS03470 (KZH43_03475) - 700445..702991 (+) 2547 WP_001149118.1 M1 family metallopeptidase -
  KZH43_RS03475 (KZH43_03480) ciaR 703100..703774 (+) 675 WP_000590640.1 two-component system response regulator CiaR Regulator
  KZH43_RS03480 (KZH43_03485) ciaH 703764..705098 (+) 1335 WP_000491790.1 two-component system sensor histidine kinase CiaH Regulator
  KZH43_RS03485 (KZH43_03490) - 705132..705419 (-) 288 WP_001145224.1 DUF3270 domain-containing protein -
  KZH43_RS03490 (KZH43_03495) - 705559..706488 (+) 930 WP_000411858.1 peptidase U32 family protein -
  KZH43_RS03495 (KZH43_03500) - 706688..709138 (+) 2451 WP_220041260.1 bifunctional DnaQ family exonuclease/ATP-dependent helicase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  ciaH htrA positive effect
  htrA comEA/celA/cilE negative effect
  htrA comC/comC1 negative effect
  comC/comC1 comD/comD1 positive effect
  comB comC/comC1 positive effect
  ciaR comC/comC1 negative effect
  ciaH comC/comC1 negative effect
  comA comC/comC1 positive effect
  comE comC/comC1 positive effect
  htrA comEC/celB negative effect
  ciaR htrA positive effect
  comD/comD1 comE positive effect
  comE comA positive effect
  comE comB positive effect
  comE comE positive effect
  comE comX/comX1 positive effect
  comE comX/comX2 positive effect
  comE comD/comD1 positive effect
  comE comM positive effect
  comE comW positive effect
  stkP comE positive effect
  comX/comX1 late competence genes positive effect
  comW comX/comX1 positive effect
  clpE comX/comX1 negative effect
  clpP comX/comX1 negative effect
  comX/comX2 late competence genes positive effect
  comW comX/comX2 positive effect
  clpE comX/comX2 negative effect
  clpP comX/comX2 negative effect
  comM cbpD negative effect
  clpC comW negative effect
  clpP comW negative effect
  mecA comW negative effect
  comX/comX1 late competence genes positive effect
  comX/comX2 late competence genes positive effect
  cbpD lytA positive effect
  cbpD lytC positive effect

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 50673.39 Da        Isoelectric Point: 9.1661

>NTDB_id=259 KZH43_RS03480 WP_000491790.1 703764..705098(+) (ciaH) [Streptococcus pneumoniae Rx1]
MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLYTSVDDKLHGLSENPQAVIQLAINRATEEIKDLENAR
ADASKVEIKPNVSSNTEVILFDKDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQSFVENASHELRTPLAVLQNRLE
TLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLNLARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRI
HRTIVTDQLLLKQLMTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKARTRQKGGF
GLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=259 KZH43_RS03480 WP_000491790.1 703764..705098(+) (ciaH) [Streptococcus pneumoniae Rx1]
ATGTTCAGTAAACTTAAAAAAACATGGTATGCGGATGACTTTAGTTATTTTATCCGCAACTTCGGTGTCTTCACCCTGAT
TTTTTCTACAATGACTCTGATTATTTTACAAGTCATGCATTCGAGTCTTTATACTTCGGTGGACGATAAGCTTCATGGAC
TGAGTGAAAATCCTCAAGCAGTTATTCAGCTGGCTATAAATAGGGCAACAGAAGAGATTAAAGATTTAGAAAATGCTAGG
GCGGACGCTAGTAAAGTAGAAATAAAACCTAATGTCAGTTCCAATACGGAAGTCATTCTCTTTGATAAGGACTTTACTCA
ACTTCTTTCTGGAAATCGATTTTTGGGCTTGGATAAGATTAAGTTAGAAAAGAAAGAACTAGGACATATCTACCAGATTC
AGGTTTTTAATAGCTATGGGCAGGAAGAAATCTATCGTGTGATTTTGATGGAGACCAATATTAGTTCGGTTTCAACCAAT
ATCAAGTATGCTGCTGTCTTGATTAATACCAGTCAGTTGGAGCAGGCTAGTCAAAAGCATGAGCAATTGATTGTGGTCGT
GATGGCTAGTTTCTGGATTTTGTCTTTACTTGCCAGTCTCTATCTAGCTAGGGTCAGTGTTAGGCCCCTGCTTGAGAGTA
TGCAGAAGCAACAGTCTTTTGTGGAAAATGCCAGTCATGAGTTACGAACTCCACTCGCAGTTTTGCAAAATCGCTTAGAG
ACCCTTTTTCGTAAGCCAGAAGCTACCATTATGGATGTGAGCGAAAGCATTGCATCGAGTTTGGAAGAAGTCCGAAATAT
GCGTTTTTTAACGACAAGCTTGCTGAACTTAGCTCGGAGAGATGATGGGATTAAGCCGGAGCTTGCAGAAGTTCCAACTA
GCTTTTTTAATACAACTTTCACAAACTATGAGATGATTGCTTCGGAAAATAATCGTGTCTTCCGTTTTGAAAATCGTATC
CATCGAACAATTGTCACAGATCAGCTTCTTCTGAAACAACTGATGACCATTCTTTTCGATAATGCCGTCAAGTATACTGA
GGAGGATGGTGAAATTGATTTTCTTATCTCGGCGACCGATCGCAATCTTTATTTACTTGTTTCTGATAATGGAATCGGTA
TTTCGACAGAAGATAAAAAGAAAATTTTTGACCGTTTTTATCGAGTAGACAAGGCTAGAACCCGGCAAAAAGGTGGTTTT
GGTTTAGGATTATCCCTAGCCAAGCAAATTGTAGATGCTCTAAAAGGAACTGTTACTGTCAAAGATAATAAACCCAAGGG
AACAATCTTTGAAGTGAAGATTGCCATTCAGACACCATCTAAAAAGAAAAAATAA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(217-283)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(327-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7B3G0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaH Streptococcus pneumoniae D39

100

100

1
  ciaH Streptococcus pneumoniae R6

100

100

1
  ciaH Streptococcus pneumoniae TIGR4

100

100

1
  ciaH Streptococcus mutans UA159

55.632

100

0.556

Multiple sequence alignment    



References

[1] Anke Laux et al. (2015) Control of competence by related non-coding csRNAs in Streptococcus pneumoniae R6. Frontiers in Genetics 6:246. [PMID: 26257773]
[2] Anke Schnorpfeil et al. (2013) Target evaluation of the non-coding csRNAs reveals a link of the two-component regulatory system CiaRH to competence control in Streptococcus pneumoniae R6. Molecular Microbiology 89(2):334-49. [PMID: 23710838]
[3] Marco Cassone et al. (2012) The HtrA protease from Streptococcus pneumoniae digests both denatured proteins and the competence-stimulating peptide. The Journal of Biological Chemistry 287(46):38449-59. [PMID: 23012372]
[4] Alexander Halfmann et al. (2011) Activity of the two-component regulatory system CiaRH in Streptococcus pneumoniae R6. Journal of Molecular Microbiology And Biotechnology 20(2):96-104. [PMID: 21422763]
[5] Miriam Müller et al. (2011) Effect of new alleles of the histidine kinase gene ciaH on the activity of the response regulator CiaR in Streptococcus pneumoniae R6. Microbiology (Reading, England) 157(Pt 11):3104-3112. [PMID: 21903754]
[6] Patrick Marx et al. (2010) Identification of genes for small non-coding RNAs that belong to the regulon of the two-component regulatory system CiaRH in Streptococcus. BMC Genomics 11:661. [PMID: 21106082]
[7] Alexander Halfmann et al. (2007) Identification of the genes directly controlled by the response regulator CiaR in Streptococcus pneumoniae: five out of 15 promoters drive expression of small non-coding RNAs. Molecular Microbiology 66(1):110-26. [PMID: 17725562]
[8] M E Sebert et al. (2005) Pneumococcal HtrA protease mediates inhibition of competence by the CiaRH two-component signaling system. Journal of Bacteriology 187(12):3969-79. [PMID: 15937159]
[9] Yasser Musa Ibrahim et al. (2004) Control of virulence by the two-component system CiaR/H is mediated via HtrA, a major virulence factor of Streptococcus pneumoniae. Journal of Bacteriology 186(16):5258-66. [PMID: 15292127]
[10] Dorothea Zähner et al. (2002) The ciaR/ciaH regulatory network of Streptococcus pneumoniae. Journal of Molecular Microbiology And Biotechnology 4(3):211-6. [PMID: 11931549]
[11] M E Sebert et al. (2002) Microarray-based identification of htrA, a Streptococcus pneumoniae gene that is regulated by the CiaRH two-component system and contributes to nasopharyngeal colonization. Infection And Immunity 70(8):4059-67. [PMID: 12117912]
[12] Philippe Giammarinaro et al. (1999) Genetic and physiological studies of the CiaH-CiaR two-component signal-transducing system involved in cefotaxime resistance and competence of Streptococcus pneumoniae. Microbiology (Reading, England) 145 ( Pt 8):1859-1869. [PMID: 10463152]