Detailed information NTG0000611

Experimentally validated

Overview

• Name: clpP
• Type: Regulator
• Locus tag: V4T04_RS07970
• Genome accession: NZ_JAZICS010000005
• Coordinates: 240687..241286 (+)
• Length: 199 a.a.
• NCBI ID: WP_003129593.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain DGCC12653
• Function: degradation of ComX Competence regulation

Function

The Clp degradation machinery plays a central role in competence repression.

Genomic Context

Location: 235687..246286

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V4T04_RS07950	-	237856..238836 (+)	981	WP_349651879.1	DUF998 domain-containing protein	-
V4T04_RS07955	-	238843..239019 (+)	177	WP_228763240.1	hypothetical protein	-
V4T04_RS07960	spx	239402..239800 (-)	399	WP_003129597.1	transcriptional regulator Spx	-
V4T04_RS07965	-	239960..240490 (-)	531	WP_003129594.1	hypothetical protein	-
V4T04_RS07970	clpP	240687..241286 (+)	600	WP_003129593.1	ATP-dependent Clp protease proteolytic subunit	Regulator
V4T04_RS07975	icd	241352..242590 (-)	1239	WP_010905501.1	NADP-dependent isocitrate dehydrogenase	-
V4T04_RS07980	acnA	242611..245157 (-)	2547	WP_349651880.1	aconitate hydratase AcnA	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
clpP	comX	negative effect
mecA	comX	negative effect
ccpA	comX	negative effect
codY	comX	negative effect
covR	comX	negative effect
covS	comX	negative effect
clpC	comX	negative effect

Sequence

Protein

• Length: 199 a.a.
• Molecular weight: 22082.24 Da
• Isoelectric Point: 4.7423

>NTDB_id=611 V4T04_RS07970 WP_003129593.1 240687..241286(+) (clpP) [Lactococcus lactis subsp. lactis strain DGCC12653]
MGYLVPTVIEQSSRGERAYDIYSRLLKDRIIMLTGPVEDGMANSIIAQLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVD
TMNFIKSDVQTIVMGMAASMGTIIASSGTKGKRFMLPNAEYLIHQPMGGTGQGTQQTDMAIVAEQLLKTRKRLEQILADN
SNRSLEQIHKDAERDHWMDAKETLEYGFIDEIMENNSLK

Nucleotide

• Length: 600 bp

>NTDB_id=611 V4T04_RS07970 WP_003129593.1 240687..241286(+) (clpP) [Lactococcus lactis subsp. lactis strain DGCC12653]
ATGGGTTATTTAGTACCTACCGTTATTGAACAATCAAGCCGTGGCGAACGTGCTTATGATATTTATTCACGACTTTTAAA
AGACCGCATTATCATGCTGACAGGACCTGTTGAAGATGGAATGGCAAATTCTATCATTGCTCAACTTCTTTTCTTGGATG
CACAAGATAACACTAAAGATATTTATCTCTACGTAAATACACCTGGTGGTTCTGTATCTGCTGGTCTTGCCATTGTTGAT
ACAATGAATTTTATCAAATCAGATGTTCAAACTATCGTTATGGGAATGGCTGCCTCTATGGGAACAATCATTGCTTCAAG
CGGAACAAAAGGCAAACGATTCATGTTGCCAAATGCTGAATATCTTATTCACCAACCAATGGGTGGTACAGGTCAAGGCA
CTCAACAAACTGATATGGCTATTGTTGCTGAGCAACTTTTGAAAACTCGTAAACGTTTGGAACAAATCCTTGCTGATAAT
TCTAATCGCTCACTTGAACAAATTCACAAAGATGCTGAACGCGACCATTGGATGGATGCCAAAGAAACTTTGGAATATGG
TTTCATCGATGAAATTATGGAAAATAATTCCTTAAAATAA

Domains

Predicted by InterproScan.

• Clp protease (CLP_protease) (14-195)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
clpP	Lactococcus lactis subsp. cremoris KW2	99.497	100	0.995
clpP	Streptococcus thermophilus LMD-9	87.245	100	0.872
clpP	Streptococcus thermophilus LMG 18311	87.245	100	0.872
clpP	Streptococcus mutans UA159	87.755	98.492	0.864
clpP	Streptococcus pyogenes JRS4	86.224	100	0.862
clpP	Streptococcus pyogenes MGAS315	86.224	100	0.862
clpP	Streptococcus pneumoniae D39	85.128	99.49	0.847
clpP	Streptococcus pneumoniae R6	85.128	99.49	0.847
clpP	Streptococcus pneumoniae TIGR4	85.128	99.49	0.847
clpP	Streptococcus pneumoniae Rx1	85.128	99.49	0.847
clpP	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	55.44	99.485	0.552
clpP	Bacillus subtilis subsp. subtilis str. 168	57.368	95.477	0.548

References

• [1]: Frédéric Toussaint et al. (2024) Unveiling the regulatory network controlling natural transformation in lactococci. PLoS Genetics 20(7):e1011340. [PMID: 38950059]