Detailed information    

experimental Experimentally validated

Overview

Name   clpP   Type   Regulator
Locus tag   SpyM6JRS4_RS01695 Genome accession   NZ_CP011414
Coordinates   325122..325712 (+) Length   196 a.a.
NCBI ID   WP_002985850.1    Uniprot ID   A0A9X8T4H8
Organism   Streptococcus pyogenes JRS4     
Function   degradation of ComX   
Competence regulation

Function

We found that expression of the sigX gene in a clpP mutant strain resulted in greater accumulation of sigma(X) protein, which resulted in higher levels of transcription from the sigma(X)-dependent promoters cinA, smf, and cglA. In addition, a clpP mutant containing sigX only at its wild-type loci on the chromosome generated more transcription from the sigma(X)-dependent cinA promoter than did the wild-type parental strain.


Genomic Context

Location: 320122..330712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SpyM6JRS4_RS01680 (SpyM6JRS4_01675) - 320935..322380 (-) 1446 WP_011184212.1 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L- lysine ligase -
  SpyM6JRS4_RS01685 (SpyM6JRS4_01680) - 322468..324102 (+) 1635 WP_011184213.1 polysaccharide biosynthesis protein -
  SpyM6JRS4_RS01690 (SpyM6JRS4_01685) upp 324269..324898 (+) 630 WP_002985854.1 uracil phosphoribosyltransferase -
  SpyM6JRS4_RS01695 (SpyM6JRS4_01690) clpP 325122..325712 (+) 591 WP_002985850.1 ATP-dependent Clp protease proteolytic subunit Regulator
  SpyM6JRS4_RS01700 (SpyM6JRS4_01695) - 326204..326479 (+) 276 WP_002985847.1 YlbG family protein -
  SpyM6JRS4_RS01705 (SpyM6JRS4_01700) tmk 326728..327363 (+) 636 WP_011184214.1 dTMP kinase -
  SpyM6JRS4_RS01710 (SpyM6JRS4_01705) - 327381..328256 (+) 876 WP_002985844.1 DNA polymerase III subunit delta' -
  SpyM6JRS4_RS01715 (SpyM6JRS4_01710) - 328275..328514 (+) 240 WP_011017422.1 hypothetical protein -
  SpyM6JRS4_RS01725 (SpyM6JRS4_01720) yabA 328918..329241 (+) 324 WP_002985838.1 DNA replication initiation control protein YabA -
  SpyM6JRS4_RS01730 (SpyM6JRS4_01725) rsmI 329246..330109 (+) 864 WP_011184217.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  SpyM6JRS4_RS01735 (SpyM6JRS4_01730) - 330136..330528 (+) 393 WP_011184218.1 DUF5684 domain-containing protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  clpP comX/sigX/comX1/sigX1 negative effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  clpP comX/sigX/comX2/sigX2 negative effect

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 21648.88 Da        Isoelectric Point: 5.2834

>NTDB_id=446 SpyM6JRS4_RS01695 WP_002985850.1 325122..325712(+) (clpP) [Streptococcus pyogenes JRS4]
MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIAQLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMN
FIKADVQTIVMGMAASMGTVIASSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
TIKQIHKDAERDYWMSAEETLAYGFIDEIMENNELK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=446 SpyM6JRS4_RS01695 WP_002985850.1 325122..325712(+) (clpP) [Streptococcus pyogenes JRS4]
ATGATTCCTGTTGTTATTGAACAAACTAGCCGTGGAGAACGTTCTTATGACATTTATTCACGTTTATTAAAAGACCGTAT
TATCATGTTAACAGGGCCTGTCGAAGACAACATGGCCAACTCTGTCATAGCGCAGCTCTTATTCCTTGACGCGCAAGACA
ACACTAAAGATATCTACCTATATGTCAACACACCAGGTGGCTCTGTCTCAGCAGGCTTGGCTATTGTCGATACCATGAAC
TTTATCAAGGCCGATGTGCAAACTATTGTCATGGGTATGGCAGCTTCTATGGGGACAGTCATTGCTTCATCAGGAACAAA
AGGCAAACGCTTTATGTTACCAAATGCAGAGTATATGATCCACCAGCCAATGGGTGGCACAGGCGGTGGCACGCAACAAA
CGGATATGGCTATCGCAGCAGAACATCTCTTAAAAACACGTCACCGTTTAGAAAAAATCTTAGCGCAAAATGCTGGAAAA
ACGATTAAACAAATCCATAAAGATGCTGAGCGTGATTATTGGATGAGTGCTGAAGAAACCTTGGCTTACGGTTTCATTGA
TGAAATCATGGAAAACAACGAACTAAAATAA

Domains

Predicted by InterProScan.

Clp protease (CLP_protease)

(11-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpP Streptococcus pyogenes MGAS315

100

100

1
  clpP Streptococcus mutans UA159

90.816

100

0.908
  clpP Streptococcus pneumoniae R6

90.769

99.49

0.903
  clpP Streptococcus pneumoniae D39

90.769

99.49

0.903
  clpP Streptococcus pneumoniae Rx1

90.769

99.49

0.903
  clpP Streptococcus pneumoniae TIGR4

90.769

99.49

0.903
  clpP Streptococcus thermophilus LMD-9

89.796

100

0.898
  clpP Streptococcus thermophilus LMG 18311

89.796

100

0.898
  clpP Lactococcus lactis subsp. lactis strain DGCC12653

86.224

100

0.862
  clpP Lactococcus lactis subsp. cremoris KW2

85.714

100

0.857
  clpP Bacillus subtilis subsp. subtilis str. 168

57.143

100

0.571
  clpP Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

56.995

98.469

0.561

Multiple sequence alignment    



References

[1] Jason A Opdyke et al. (2003) Expression of the secondary sigma factor sigmaX in Streptococcus pyogenes is restricted at two levels. Journal of Bacteriology 185(15):4291-7. [PMID: 12867436]