Detailed information NTG0001223

Experimentally validated

Overview

• Name: clpP
• Type: Unclear
• Locus tag: Cj0192c
• Genome accession: NC_002163
• Coordinates: 187535..188119 (-)
• Length: 194 a.a.
• NCBI ID: YP_002343650.1
• Uniprot ID: P54413
• Organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
• Function: require for natural transformation Unclear

Function

The ATP dependent ClpP protease to be important for transformation, which possibly could be associated with reduced protein glycosylation in the ClpP mutant.

Genomic Context

Location: 182535..193119

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Cj0187c	purN	182859..183425 (-)	567	YP_002343645.1	phosphoribosylglycinamide formyltransferase	-
Cj0188c	-	183416..184786 (-)	1371	YP_002343646.1	kinase	-
Cj0189c	-	184770..185468 (-)	699	YP_002343647.1	hypothetical protein	-
Cj0190c	-	185470..186975 (-)	1506	YP_002343648.1	hypothetical protein	-
Cj0191c	def	186972..187499 (-)	528	YP_002343649.1	peptide deformylase	-
Cj0192c	clpP	187535..188119 (-)	585	YP_002343650.1	ATP-dependent Clp protease proteolytic subunit	Unclear
Cj0193c	tig	188119..189453 (-)	1335	YP_002343651.1	trigger factor	-
Cj0194	folE	189596..190168 (+)	573	YP_002343652.1	GTP cyclohydrolase I	-
Cj0195	fliI	190168..191553 (+)	1386	YP_002343653.1	flagellum-specific ATP synthase	-
Cj0196c	purF	191556..192893 (-)	1338	YP_002343654.1	amidophosphoribosyltransferase	-

Sequence

Protein

• Length: 194 a.a.
• Molecular weight: 21650.14 Da
• Isoelectric Point: 7.1074

>NTDB_id=1223 Cj0192c YP_002343650.1 187535..188119(-) (clpP) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
MFIPYVIEKSSRGERSYDIYSRLLKDRIIMLSGEIHDELAASIVAQLLFLEAEDPTKDIYLYINSPGGVITSGFSIYDTM
NYIKPDVCTICIGQAASMGAFLLSCGAEGKRFALPNSRIMIHQPLGGARGQATDIEIQAKEILRLKTILNDILAKNTKQK
VAKIAKDTERDFFMSAQEAKEYGLIDKVLEKSFK

Nucleotide

• Length: 585 bp

>NTDB_id=1223 Cj0192c YP_002343650.1 187535..188119(-) (clpP) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
ATGTTTATTCCTTATGTTATTGAAAAATCTAGTCGTGGTGAAAGAAGTTATGACATTTATTCACGCCTTTTAAAAGATAG
AATTATTATGCTAAGTGGTGAAATTCATGATGAACTTGCCGCTTCTATCGTAGCACAACTTCTTTTTTTAGAAGCTGAAG
ATCCTACAAAAGATATTTATCTTTATATCAATTCTCCAGGTGGTGTAATTACAAGTGGTTTTAGTATTTATGATACTATG
AACTATATCAAACCTGATGTTTGTACGATTTGTATAGGACAAGCTGCTTCTATGGGAGCATTTTTGCTTAGTTGTGGCGC
AGAAGGAAAACGTTTTGCTTTGCCTAATTCACGTATTATGATTCATCAACCTTTAGGTGGAGCTAGAGGTCAAGCCACAG
ATATTGAAATTCAAGCCAAAGAAATTTTAAGACTCAAAACTATACTTAATGATATTTTGGCAAAAAATACTAAGCAAAAA
GTTGCAAAAATAGCAAAAGATACAGAAAGAGATTTTTTCATGTCAGCACAAGAAGCTAAAGAATATGGGCTGATTGATAA
GGTTTTGGAAAAAAGTTTTAAATGA

Domains

Predicted by InterproScan.

• Clp protease (CLP_protease) (12-191)

3D structure

• AlphaFold DB: P54413

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
clpP	Bacillus subtilis subsp. subtilis str. 168	68.617	96.907	0.665
clpP	Streptococcus thermophilus LMG 18311	58.549	98.469	0.577
clpP	Streptococcus thermophilus LMD-9	58.549	98.469	0.577
clpP	Streptococcus pneumoniae TIGR4	58.031	98.469	0.571
clpP	Streptococcus pneumoniae R6	58.031	98.469	0.571
clpP	Streptococcus pneumoniae Rx1	58.031	98.469	0.571
clpP	Streptococcus pneumoniae D39	58.031	98.469	0.571
clpP	Lactococcus lactis subsp. cremoris KW2	56.995	99.485	0.567
clpP	Streptococcus pyogenes JRS4	56.995	98.469	0.561
clpP	Streptococcus pyogenes MGAS315	56.995	98.469	0.561
clpP	Streptococcus mutans UA159	55.959	99.485	0.557
clpP	Lactococcus lactis subsp. lactis strain DGCC12653	55.44	99.485	0.552

References

• [1]: Christina S Vegge et al. (2012) Natural transformation of Campylobacter jejuni occurs beyond limits of growth. PloS One 7(9):e45467. [PMID: 23049803]