Detailed information    

experimental Experimentally validated

Overview

Name   mecA   Type   Regulator
Locus tag   V4T04_RS04795 Genome accession   NZ_JAZICS010000003
Coordinates   177685..178386 (-) Length   233 a.a.
NCBI ID   WP_349651757.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain DGCC12653     
Function   degradation of ComX   
Competence regulation

Function

MecA plays a crucial role in competence repression


Genomic Context

Location: 172685..183386
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4T04_RS04775 - 172795..174012 (-) 1218 WP_033899923.1 cysteine desulfurase -
  V4T04_RS04780 sufD 174012..175268 (-) 1257 WP_349651755.1 Fe-S cluster assembly protein SufD -
  V4T04_RS04785 sufC 175406..176176 (-) 771 WP_004255181.1 Fe-S cluster assembly ATPase SufC -
  V4T04_RS04790 - 176351..177682 (-) 1332 WP_349651756.1 glycosyltransferase family 4 protein -
  V4T04_RS04795 mecA 177685..178386 (-) 702 WP_349651757.1 adaptor protein MecA Regulator
  V4T04_RS04800 - 178516..179250 (-) 735 WP_349651758.1 amino acid ABC transporter ATP-binding protein -
  V4T04_RS04805 - 179250..179936 (-) 687 WP_004255158.1 amino acid ABC transporter permease -
  V4T04_RS04810 - 180082..180990 (-) 909 WP_003130548.1 diacylglycerol/lipid kinase family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  mecA comX negative effect
  clpP comX negative effect
  ccpA comX negative effect
  codY comX negative effect
  covR comX negative effect
  covS comX negative effect
  clpC comX negative effect

Sequence

Protein


Download         Length: 233 a.a.        Molecular weight: 27120.49 Da        Isoelectric Point: 4.1605

>NTDB_id=609 V4T04_RS04795 WP_349651757.1 177685..178386(-) (mecA) [Lactococcus lactis subsp. lactis strain DGCC12653]
MKYEDINENTIKITLSFDDLTDYDIKLSDFFGNQEVIEQFFYELVDELGLENRFGNVGMLTFQIQPFPQGVHMIVHEEAM
LGEGGEIPDDPEEFEELMTGFYNKLNEIGADMARERGITDFKPGRGLPGTKKDEAEQEPDFIYYSIRYEDIMSVLTGIKN
VKFADEESEFYRYDGNFYLVVLDNQKEKGKMHVESTRSRMMEYGEATKMSREFLQEYGECLIATRALDVLRKI

Nucleotide


Download         Length: 702 bp        

>NTDB_id=609 V4T04_RS04795 WP_349651757.1 177685..178386(-) (mecA) [Lactococcus lactis subsp. lactis strain DGCC12653]
ATGAAATATGAGGATATAAACGAAAATACTATAAAAATCACCTTGTCTTTTGATGATTTGACAGATTATGATATCAAATT
ATCAGACTTTTTCGGAAATCAAGAAGTCATTGAACAATTTTTCTATGAATTGGTTGATGAGCTTGGTTTAGAAAATCGCT
TTGGAAATGTGGGAATGTTAACTTTCCAAATCCAACCTTTTCCTCAAGGTGTTCATATGATTGTTCATGAAGAAGCGATG
CTAGGTGAAGGTGGAGAAATTCCAGATGACCCAGAAGAGTTTGAAGAATTGATGACTGGTTTTTATAATAAATTAAATGA
AATAGGGGCAGATATGGCGCGCGAGCGAGGAATTACTGATTTTAAACCTGGGCGTGGTTTACCAGGAACAAAAAAAGATG
AAGCTGAACAAGAGCCAGATTTTATTTATTACTCTATTCGTTATGAAGATATTATGTCTGTCTTAACAGGAATAAAAAAT
GTGAAGTTTGCTGATGAAGAATCAGAGTTTTATCGTTATGATGGCAATTTTTATCTTGTTGTTTTGGATAATCAAAAAGA
AAAAGGCAAGATGCATGTTGAAAGTACCCGTTCACGAATGATGGAATATGGGGAAGCAACAAAAATGAGTCGAGAATTTT
TGCAGGAGTATGGTGAATGTCTTATCGCGACACGTGCTTTAGATGTTCTTAGAAAAATCTAA

Domains

Predicted by InterproScan.

Negative regulator of genetic competence (MecA) (MecA)

(1-230)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mecA Lactococcus lactis subsp. cremoris KW2

96.567

100

0.966

Multiple sequence alignment    



References

[1] Frédéric Toussaint et al. (2024) Unveiling the regulatory network controlling natural transformation in lactococci. PLoS Genetics 20(7):e1011340. [PMID: 38950059]